Effects of a Composite Endomycorrhizal Inoculum on Olive Cuttings under the Greenhouse Conditions

This study was carried out in a nursery to evaluate the impact of mycorrhizal fungi on the cutting's root growth, and root colonization of a Moroccan olive variety ‘Picholine Marocaine' under greenhouse conditions during 2 years of cultivation. The results revealed that the inoculation with a composite inoculum of arbuscular mycorrhizal fungi (AMF) stimulated an early root formation and high development of vegetative shoots in inoculated cuttings respectively, 35 days (50 days in the control plots) and 40 days (60 days in the control plots) after their culture. The progressive establishment of mycorrhizal symbiosis in the roots of the inoculated plants showed that the root and vegetative masses were respectively 24 g and 19.5 g two years after inoculation. The average height and the leave's number of the inoculated plants relative to the control were respectively s 42/ 12 cm and 145/12. The newly formed roots were mycorrhizal and present different structures characteristic of AMF: arbuscules, vesicles, hyphae and spores, whose frequency and intensity reached 90% and 75% two years after cuttings cultivation. The arbuscular and vesicular contents and the number of spores were 67%, 96% and 212 spores/ 100 g of soil respectively. The fourteen species of mycorrhizal fungi isolated from the rhizosphere belong to 4 genera (Glomus, Acaulospora, Gigaspora, and Scutellospora) and three families (Glomaceae, Acaulosporaceae and Gigasporacea).The Glomus genus was the most dominant (65%) followed by the Gigaspora genus (22%). Glomus intraradices, Gigaspora sp.2, Glomus versiformes are the most abundant species, their frequency of occurrence are respectively 30%, 21% and 16%