概日リズムはマウス接触過敏反応の強度に影響を与える

京都大学新制・課程博士博士(医学)甲第24133号医博第4873号新制||医||1059(附属図書館)京都大学大学院医学研究科医学専攻(主査)教授 生田 宏一, 教授 濵﨑 洋子, 教授 森信 暁雄学位規則第4条第1項該当Doctor of Medical ScienceKyoto UniversityDFA

A Phenotypic Analysis of Involucrin-Membrane-Bound Ovalbumin Mice after Adoptive Transfer of Ovalbumin-Specific CD8⁺ T Cells

To investigate the mechanism of autoimmunity and peripheral tolerance in the skin, several transgenic mouse strains expressing membrane-bound ovalbumin (mOVA) as an epidermal self-antigen under the control of keratinocyte-specific promotors, such as keratin 5 and keratin 14, were employed in combination with adoptive transfer of CD8⁺ T cells from OT-I mice (OT-I T cells) that recognize an ovalbumin-derived peptide. However, these strains showed bodyweight loss and required additional inflammatory stimuli, such as γ-irradiation and tape-stripping, to induce skin inflammation. In this study, we generated a mouse strain expressing mOVA under the control of human involucrin promoter (involucrin-mOVA mice). In contrast to previous strains, involucrin-mOVA mice spontaneously developed skin inflammation after the transfer of OT-I T cells in the absence of external stimuli without significant bodyweight loss. We focused on the skin infiltration process of OT-I T cells and found that transferred OT-I T cells accumulated around the hair follicles in the early phase of skin inflammation, and in the later phase, the skin inflammation spontaneously resolved despite the remaining OT-I T cells in the skin. Our involucrin-mOVA mice will provide a promising tool to investigate the pathogenesis and the tolerance mechanisms of cytotoxic skin autoimmunity

Annealing to achieve lower resistivity in Ga-doped ZnO epitaxial films grown from low-temperature aqueous solution

Various annealing conditions were examined for enhancing the electrical conductivity of gallium-doped ZnO (ZnO:Ga) epitaxial films grown from a low-temperature aqueous solution. The resistivity of the films decreased with increasing annealing temperature, accompanying increases in both the carrier concentration and mobility. However, the improvement obtained from 0.5 h of annealing was limited because the films spalled when the temperature exceeded ∼300 °C. The temperature at which spalling of the films occurred depended on the annealing atmosphere, and it was lower in 5%H₂–Ar than in air or vacuum. Thermal desorption spectroscopy showed that the cause of spalling was expulsion of H₂O vapor from the interior of the film, indicating that water molecules or hydroxides were incorporated into the crystal during film growth. Analysis of the variations in electrical properties with increasing annealing duration revealed that the electrical properties were quickly improved upon heating before the film spalled. Thus, by limiting the annealing duration to ≤20 s, annealing could be performed at a temperature as high as 580 °C without spalling of the film, which yielded the lowest resistivity of 4.7 × 10⁻⁴ Ω cm. The annealed film exhibited transmittance as high as >75% in the visible spectrum

Spectral Based Color Correction Technique Compatible with

A spectral turn method is presented as a spectral based color correction method compatible with the standard RGB system by applying the idea of a multi-spectral method. The multi-spectral based color reproduction technique has been proposed for providing independent color of device and viewing conditions. However, it is difficult to replace the RGB devices with high cost multi-spectral devices since the latter have advanced only to the laboratory level. In the proposed method, the standard RGB values are converted into spectral information, then returned to RGB values for different viewing conditions. This process provides more accurate reproduction than the conventional forward process of the color reproduction. An experiment of psychophysical color matching was performed to evaluate the technique. The mixed chromatic adaptation model was applied to the method in the experiment, and the results showed the effectiveness of the proposed method

Syringocystadenocarcinoma Papilliferum in the Perianal Area

Syringocystadenocarcinoma papilliferum (SCACP) is a very rare cutaneous adnexal neoplasm. SCACP presents histologic variability, and it is difficult to establish the diagnosis from a punch biopsy. SCACP has an overall configuration similar to that of syringocystadenoma papilliferum (SCAP). When we diagnose SCACP, the histologic features of SCAP can be contributing and immunohistochemical staining is useful. Our case shows the histologic variability of SCACP and the pitfalls of a punch biopsy for the diagnosis of SCACP