A global profile of replicative polymerase usage

Three eukaryotic DNA polymerases are essential for genome replication. Polymerase (Pol) α–primase initiates each synthesis event and is rapidly replaced by processive DNA polymerases: Polɛ replicates the leading strand, whereas Polδ performs lagging-strand synthesis. However, it is not known whether this division of labor is maintained across the whole genome or how uniform it is within single replicons. Using Schizosaccharomyces pombe, we have developed a polymerase usage sequencing (Pu-seq) strategy to map polymerase usage genome wide. Pu-seq provides direct replication-origin location and efficiency data and indirect estimates of replication timing. We confirm that the division of labor is broadly maintained across an entire genome. However, our data suggest a subtle variability in the usage of the two polymerases within individual replicons. We propose that this results from occasional leading-strand initiation by Polδ followed by exchange for Polɛ

Two-particle interference of electron pairs on a molecular level

We investigate the photo-doubleionization of $H_2$ molecules with 400 eV photons. We find that the emitted electrons do not show any sign of two-center interference fringes in their angular emission distributions if considered separately. In contrast, the quasi-particle consisting of both electrons (i.e. the "dielectron") does. The work highlights the fact that non-local effects are embedded everywhere in nature where many-particle processes are involved

Schizosaccharomyces pombe Cds1 regulates homologous recombination at stalled replication forks through the phosphorylation of recombination protein Rad60

The Schizosaccharomyces pombe rad60 gene is essential for cell growth and is involved in repairing DNA double-strand breaks. Rad60 physically interacts with, and is functionally related to, the structural maintenance of chromosomes 5 and 6 protein complex (Smc5/6). Rad60 is phosphorylated in response to hydroxyurea (HU)-induced DNA replication arrest in a Cds1Chk2- dependent manner. Rad60 localizes in nucleus in unchallenged cells, but becomes diffused throughout the cell in response to HU. To understand the role of Rad60 phosphorylation, we mutated the putative phosphorylation target motifs of Cds1Chk2 and have identified two Cds1Chk2 target residues responsible for Rad60 dispersal in response to HU. We show that the phosphorylationdefective rad60 mutation partially suppresses HU sensitivity and the elevated recombination frequency of smc6-X. Our data suggest that Rad60 phosphorylation is required to regulate homologous recombination at stalled replication forks, probably by regulating Smc5/6. Supplementary material available online at http://jcs.biologists.org/cgi/content/full/122/20/3638/DC

On Martin-Löf convergence of Solomonoff’s mixture

We study the convergence of Solomonoff’s universal mixture on individual Martin-Löf random sequences. A new result is presented extending the work of Hutter and Muchnik (2004) by showing that there does not exist a universal mixture that converges on all Martin-Löf random sequences

Photoproduction of Lambda(1405) and Sigma^{0}(1385) on the proton at E_\gamma = 1.5-2.4 GeV

Differential cross sections for $\gamma p \to K^+\Lambda(1405)$ and $\gamma p \to K^+\Sigma^0(1385)$ reactions have been measured in the photon energy range from 1.5 to 2.4 GeV and the angular range of $0.8<\cos(\Theta)<1.0$ for the $K^+$ scattering angle in the center-of-mass system. This data is the first measurement of the $\Lambda(1405)$ photoproduction cross section. The lineshapes of \LamS measured in $\Sigma^+\pi^-$ and $\Sigma^-\pi^+$ decay modes were different with each other, indicating a strong interference of the isospin 0 and 1 terms of the $\Sigma\pi$ scattering amplitudes. The ratios of \LamS production to \SigS production were measured in two photon energy ranges: near the production threshold ($1.5<E_\gamma<2.0$ GeV) and far from it ($2.0 <E_\gamma<2.4$ GeV). The observed ratio decreased in the higher photon energy region, which may suggest different production mechanisms and internal structures for these hyperon resonances