1 research outputs found
Not Available
Not AvailableNucellar embryogenesis was induced in Mangifera indica
L. cv. Kurakkan, a polyembryonic salt tolerant, dwarfing
rootstock. Nucellus tissue excised from 3.5 cm long fruits
developed pro-embryonic callus in 19 days of inoculation
on modified MS medium supplemented with 4.52µM 2,4-
D, 0.05% malt extract and 13.78µM spermidine. Somatic
embryogenesis exhibited high frequency (158.33
embryos). However, all the differentiated embryos
proliferated on medium having low level of sucrose (4%
w/v) and auxin (2.26µM 2,4-D). Most of the proembryonic
calli converted into heart shaped and cotyledonary
embryos by reducing temperature to 15
o
C. Somatic
embryos were matured on modified MS medium fortified
with 0.38µM ABA, 0.57µM IAA and 30.30µM PEG. Matured
somatic embryos germinated (around 30%) on MS medium
supplemented with 2.68µM NAA, 11.60µM kinetin and
2736.9µM glutamine.Not Availabl