VISION SYSTEM DEVELOPMENT IN ECCENTRICITY TESTING FOR KWH METER UPPER BEARING

Eccentricity measurement techniques are developed to ensure optimization of operational works, either for large or small objects such as upper bearing kWH meter. Small object eccentricity testing has been done by using precise and sensitive touch trigger probing and a special sofware that makes it expensive. This study developed a more economical vision systemin eccentricity testing. It consists of bearing holder and CCD microscope as an image recorder and computerized image processing. It uses three tested bearing during image recording process, which obtained 12 images from each tested bearing taken from every 30o angle, and one image as registration reference. Image registration process is conducted to correct imperfections bearing mounting into its holder and use centroid method to test the eccentricity of upper bearing kWH meter. This study is succeed in making an eccentricity testing prototype of upper bearing kWH meter which obtained result of 2nd bearing has its largest standard deviation. From all standard deviation value obtained that x -axis (horizontal) standard deviation is larger than y-axis (vertical) which means that detection of x direction is more accurate than y direction. To enhance precision image acquisition in the next study, it is expected the use of a computer system with homogeneous illumination is enabled

Prescription audit of corticosteroid usage in the department of dermatology at a tertiary care teaching hospital

Background: Corticosteroids are a group of commonly used drugs in dermatology practice whose non judicious use frequently results in undesirable and unwanted effects. Prescribing them rationally with care allows us to derive the maximum benefit out of them with minimal side effects.Methods: Retrospective analysis of 112 case files belonging to patients admitted in the department of dermatology over a period of one year was undertaken to ascertain the usage pattern of corticosteroids in relation to their potency, strength, frequency, duration, route, quantity to be applied. The data thus collected was expressed in terms of averages, ratios and proportions.Results: The total number of formulations prescribed were 929 out of which 10.8% were corticosteroids. The average number of formulations prescribed per patient was 8.29. About 78(70%) patients admitted in dermatology received corticosteroids. Topical steroids were prescribed in 50 patients (44.7 %) out of whom 36 (72%) received only topical, the remaining 14(28%) were prescribed both systemic and topical corticosteroids.Brand names were used in all cases. Highly potent corticosteroids like clobetasol, halobetasol and mometasone were prescribed to 39(50%) of all cases who received topical corticosteroids.Conclusion: The study reveals the deficiencies which exist in the present prescribing pattern of corticosteroids. Educational interventions among the doctors as well as students should be carried out to in order to promote rational drug use

Pattern of drug use in psoriasis inpatients in the department of dermatology at a tertiary care teaching hospital

Background: The pattern of drug use in people hospitalized with a primary diagnosis of psoriasis has never been studied previously in India. The aim of the study was to characterize the prescription pattern of people admitted to hospital with psoriasis so that rational prescribing could be promoted among dermatologists.Methods: Case files belonging to 32 patients, admitted in the Department of Dermatology with psoriasis, of a tertiary care teaching hospital in Kerala, over a period of 1-year were retrieved with the assistance of the medical records department. The data thus obtained was analyzed using descriptive statistics.Results: The average age of patients who were admitted with psoriasis was 49.9 years, and their average duration of hospital stay was 7.96 days. A total of 296 formulations were prescribed to 32 patients. Out of 296 formulations, only 10 (3.37%) were generic and rest 286 (96.62%) branded. Fixed dose combinations consisted of 32.43% (96/296) of the prescribed formulations. Psoriasis vulgaris (56.25%) was the most common cause for admission. Of all the prescribed medications, 4 (1.35%) did not contain clear instructions for the route of administration. Strength was clearly mentioned in only 89 (30%) of the preparations. In 98% of the prescriptions, the exact dose was missing.Conclusions: The study reveals various deficiencies which exist in the prescribing pattern of drugs for management of psoriasis. Educational interventions among the doctors as well as students should be carried out to promote rational drug use.

THE GUT MICROBIOTA REVOLUTION: AN OVERVIEW OF FECAL MICROBIOTA TRANSPLANTATION FOR GASTROINTESTINAL DISORDERS

Abstract The gut microbiota revolution has emerged as a significant breakthrough in understanding the intricate relationship between the microbial community residing in the human gut and its impact on various aspects of human health. Among the notable advances in this field, fecal microbiota transplantation (FMT) has gained considerable attention as a promising therapeutic approach for gastrointestinal disorders. This review provides an overview of FMT, its historical background, methodology, and its potential applications in the treatment of gastrointestinal disorders. FMT involves the transfer of fecal material with the aim of restoring a balanced and diverse gut microbiota. FMT is most commonly utilized for the management of recurrent Clostridioides difficile infection. Clinical studies investigating the efficacy of FMT have shown promising results, particularly in the management of recurrent Clostridioides difficile infection. The restoration of a healthy gut microbiota through FMT appears to promote microbial diversity, improve microbial functions, and modulate the host immune response. However, challenges remain, including standardization of the procedure, long-term safety monitoring, and optimizing donor selection and preparation protocols. In conclusion, fecal microbiota transplantation represents a revolution in the field of gut microbiota research and offers a promising therapeutic avenue for gastrointestinal disorders. While the evidence base continues to evolve, FMT holds significant potential to transform the management of various conditions by targeting the root cause of dysbiosis. Continued research and clinical trials are warranted to establish guidelines, refine protocols, and expand the applications of FMT in gastrointestinal medicine

Borax Detection Tool in Foodstuffs Using Color Recognition Detection Method on HuskyLens Camera

The use of Borax as a food additive has adverse effects on public health. In an effort to prevent contaminated foodstuffs from becoming public consumption and to detect this contamination quickly and effectively, this research proposes the design of an Internet of Things (IoT)-based borax detection tool using color recognition technology from the HuskyLens Camera. This tool is designed to detect the presence of borax in food with high accuracy and in a short time. This research uses the HuskyLens Camera as a sensor that is sensitive to the target contaminant, and the data obtained from this sensor is transmitted through the IoT network to the MIT App Inventor platform. Optimized detection methods and data processing algorithms are used to accurately interpret the sensor results, providing authorities with immediate information on the safety status of the tested foodstuffs. Experimental test results show that the proposed detection tool has good performance in detecting borax in various types of foodstuffs. It provides an efficient and reliable solution to the challenge of food contamination. With the integration of IoT technology, the detector is able to provide real-time information to authorities, potentially raising awareness about overall food safety. Thus, the design of this IoT-based borax detector has great potential to overcome the problem of food contamination and make a significant contribution to maintaining public health

Loop 2 in Saccharomyces cerevisiae Rad51 protein regulates filament formation and ATPase activity

Previous studies showed that the K342E substitution in the Saccharomyces cerevisiae Rad51 protein increases the interaction with Rad54 protein in the two-hybrid system, leads to increased sensitivity to the alkylating agent MMS and hyper-recombination in an oligonucleotide-mediated gene targeting assay. K342 localizes in loop 2, a region of Rad51 whose function is not well understood. Here, we show that Rad51-K342E displays DNA-independent and DNA-dependent ATPase activities, owing to its ability to form filaments in the absence of a DNA lattice. These filaments exhibit a compressed pitch of 81 Å, whereas filaments of wild-type Rad51 and Rad51-K342E on DNA form extended filaments with a 97 Å pitch. Rad51-K342E shows near normal binding to ssDNA, but displays a defect in dsDNA binding, resulting in less stable protein-dsDNA complexes. The mutant protein is capable of catalyzing the DNA strand exchange reaction and is insensitive to inhibition by the early addition of dsDNA. Wild-type Rad51 protein is inhibited under such conditions, because of its ability to bind dsDNA. No significant changes in the interaction between Rad51-K342E and Rad54 could be identified. These findings suggest that loop 2 contributes to the primary DNA-binding site in Rad51, controlling filament formation and ATPase activity

Differential Requirements of Two recA Mutants for Constitutive SOS Expression in Escherichia coli K-12

Background Repairing DNA damage begins with its detection and is often followed by elicitation of a cellular response. In E. coli, RecA polymerizes on ssDNA produced after DNA damage and induces the SOS Response. The RecA-DNA filament is an allosteric effector of LexA auto-proteolysis. LexA is the repressor of the SOS Response. Not all RecA-DNA filaments, however, lead to an SOS Response. Certain recA mutants express the SOS Response (recAC) in the absence of external DNA damage in log phase cells. Methodology/Principal Findings Genetic analysis of two recAC mutants was used to determine the mechanism of constitutive SOS (SOSC) expression in a population of log phase cells using fluorescence of single cells carrying an SOS reporter system (sulAp-gfp). SOSC expression in recA4142 mutants was dependent on its initial level of transcription, recBCD, recFOR, recX, dinI, xthA and the type of medium in which the cells were grown. SOSC expression in recA730 mutants was affected by none of the mutations or conditions tested above. Conclusions/Significance It is concluded that not all recAC alleles cause SOSC expression by the same mechanism. It is hypothesized that RecA4142 is loaded on to a double-strand end of DNA and that the RecA filament is stabilized by the presence of DinI and destabilized by RecX. RecFOR regulate the activity of RecX to destabilize the RecA filament. RecA730 causes SOSC expression by binding to ssDNA in a mechanism yet to be determined

Pemanfaatan Modal Sosial Sebagai Strategi Organisasi Pengelola Zakat Dalam Menghim Dana Di Masyarakat (Studi Kasus : Lazismu Kantor Layanan Masjid Tawea Muhammadiyah)

Organisasi Pengelola Zakat (OPZ) merupakan organisasi yang bertugas menghimpun potensi dana zakat yang ada di masyarakat. Sebagai sebuah OPZ Lazismu Kantor Layanan Masjid Taqwa Muhammadiyah harus memiliki strategi dalam menjalankan tugasnya. Tujuan umum penelitian ini adalah mendeskripsikan strategi Lazismu Kantor Layanan (KL) Masjid Taqwa Muhammadiyah, Sumatra Barat dalam menghimpun dana masyarakat. Sedangkan tujuan khusus dari penelitian ini adalah mendeskripsikan pengelolaan dana zakat yang dikumpulkan oleh Lazismu KL Masjid Taqwa Muhammadiyah, mendeskripsikan upaya-upaya yang dilakukan Lazismu KL Masjid Taqwa Muhammadiyah dalam menghimpun dana zakat, serta mendeskripsikan pemanfaatan modal sosial sebagai strategi Lazismu KL Masjid Taqwa Muhammadiyah dalam menghimpun dana zakat. Penelitian ini menggunakan teori modal sosial dari Robert MZ Lawang. Pendekatan penelitian yang digunakan untuk mendapatkan data adalah dengan metode kualitatif, yang mana data yang diperoleh dari wawancara mendalam, observasi, dan pengumpulan data sekunder. Pemilihan informan dilakukan dengan teknik Purposive Sampling. Unit analisisnya adalah Lazismu KL Masjid Taqwa Muhammadiyah. Berdasarkan hasil penelitian ditemukan bentuk pemanfaatan modal sosial sebagai strategi Lazismu KL Masjid Taqwa Muhammadiyah dalam menghimpun dana. Jaringan sosial yang ada mampu dimanfaatkan dengan sangat baik oleh Lazismu KL Masjid Taqwa Muhammadiyah. Lazismu KL Masjid Taqwa Muhammadiyah juga dapat membangun kepercayaan dengan para muzzaki hingga membangun hubungan yang berkelanjutan. Norma dapat mengatur Lazismu KL Masjid Taqwa Muhammadiyah dalam menjalankan tugasnya