Djelotvornost pripravka MCC® kao konačnog dekontaminata sumporova iperita

The aim of this study was to evaluate decontamination (absorption) efficacy of a preparation called Mineral Cationic Carrier (MCC®) against skin contamination with sulphur mustard in vivo. MCC® is a synthetic preparation with known ion exchange, absorption efficiency, and bioactive potential. CBA mice were applied increasing doses of sulphur mustard on their skin and MCC® was administered immediately after skin contamination. The results have confirmed the decontamination efficacy of MCC® preparation, corresponding to 8.4 times the LD50 of percutaneous sulphur mustard, and call for further investigation.Cilj ovog istraživanja bio je ispitati dekontaminacijska (adsorpcijska) svojstva pripravka MCC® (Mineral Cationic Carrier) rabeći kožni bojni otrov iperit kao kontaminant u uvjetima in vivo. MCC® je sintetski pripravak koji je biokemijski aktivan i ima ionskoizmjenjivačka i adsorpcijska svojstva. Istraživanje u uvjetima in vivo provedeno je na miševima (soj CBA), aplikacijom rastućih doza iperita na kožu životinje. Pripravak MCC® uporabljen je kao kožni dekontaminant neposredno nakon perkutane kontaminacije iperitom. Rezultati istraživanja pokazuju da se dekontaminacijom pripravkom MCC® može postići terapijski učinak od 8,4 LD50 (perkutano, iperit). Dobiveni rezultati potvrđuju vrlo dobru dekontaminacijsku učinkovitost pripravka MCC® i govore u prilog daljnjim istraživanjima s ciljem njegove moguće šire primjene

Inhibitory effect of the plant proteins mixture on the specific sucrase activity in intestine of diabetic mice

Background and Purpose: Increased activity of sucrase, one of the intestinal alpha-glucosidase founded in diabetes mellitus. Inhibition of sucrase activity, plays a major role in preventing rise in postprandial glucose level in diabetics. On peer-reviewed literature could be found regarding investigation the effect of mixture plant proteins, Mw 3 – 15 kDa (MPP), isolated from Astragali radix – Astragalus membranaceus Fisch., Foenugraeci semen – Trigonella foenum graecum L., Cichorii radix – Cichorium Intybus L. and Urticae radix and herba – Urtica dioica L. on sucrase activity. This plants are used in traditional medicine of treatment of diabetes mellitus. The aim of this study was to determine activity of sucrase in small intestinal homogenates of NOD diabetic mice on feeding with and without MPP in chow. Materials and Methods: In mice diabetes was induced by i.v. injection of aloxan-monohydrate (75 mg/kg b.m.) seven days before treatment with MPP. The proteins (Mw 3 – 15 kDa), were isolated from ethanol extract, each plants separately, by gel filtration method on Sephadex G-25 column. Eluted fraction which highest absorbance on 280 nm were pooled, dialyzed, lyophilized and mixed (MPP) and before treatment in mice solvent in sterile PBS. After seven days of treatment diabetic NOD mice with MPP (1,8 g/d), the small intestine was removed and divided into three segments, from pylorus to duodenum, and two equal lengths of the jejunum and ileum and homogenized in cold 0.14M KCl. Specific sucrase activity was determined using method of Dahlquist et. al., by sucrose as substrate. Results and Conclusion: We confirmed the increased specific sucrase activity in the intestine of diabetic NOD mice. Our results also indicate that MPP have strongly inhibitory potential on intestinal sucrase activity (p<0.05) in diabetic mice. Conclusions drawn from this study should be further supported and our future experiments will be focused on determining the amino acid sequence of each protein from MPP

Toxicological Assessment of P-9801091 Plant Mixture Extract after Chronic Administration in CBA/HZg Mice – A Biochemical and Histological Study

Acute, subchronic and chronic effects of the P-9801091 plant mixture extract at a dose of 20 mg/kg body mass were assessed in serum of healthy CBA/HZg mice at 24 hours, 7 days, 3 months and 6 months of treatment (experimental group), and compared with the values obtained in the control group of untreated healthy CBA/HZg mice. The P-9801091 plant mixture extract is an antihyperglycemic preparation containing Myrtilli folium (Vaccinium myrtillus L.), Taraxaci radix (Taraxacum officinale Web.), Cichorii radix (Cichorium intybus L.), Juniperi fructus (Juniperus communis L.), Centaurii herba (Centaurium umbellatum Gilib.), Phaseoli fructus sine semine (Phaseolus vulgaris L.), Millefolii herba (Achillea millefolium L.), Mori folium (Morus nigra L.), Valerianae radix (Valeriana officinalis L.) and Urticae herba et radix (Urtica dioica L). Toxic effect of the P-9801091 plant mixture extract was assessed by the following biochemical parameters: urea, creatinine, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and cholesterol. Also, histopathological examination of the kidneys, liver, spleen, pancreas, testes and lungs was performed. Results of biochemical testing performed at specified time points generally showed no statistically significant differences from control values, with the only exception of the catalytic concentration of AST in the experimental group measured on day 7, which was significantly increased as compared with the control group (p<0.05). Pathohistological examination including characteristic organ and tissue structure, and parenchyma relationship to the adjacent blood vessels and connective tissue in the examined organs revealed no major pathologic changes

Submicron mass spectrometry imaging of single cells by combined use of mega electron volt time-of-flight secondary ion mass spectrometry and scanning transmission ion microscopy

In order to better understand biochemical processes inside an individual cell, it is important to measure the molecular composition at the submicron level. One of the promising mass spectrometry imaging techniques that may be used to accomplish this is Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS), using MeV energy heavy ions for excitation. MeV ions have the ability to desorb large intact molecules with a yield that is several orders of magnitude higher than conventional SIMS using keV ions. In order to increase the spatial resolution of the MeV TOF-SIMS system, we propose an independent TOF trigger using a STIM (scanning transmission ion microscopy) detector that is placed just behind the thin transmission target. This arrangement is suitable for biological samples in which the STIM detector simultaneously measures the mass distribution in scanned samples. The capability of the MeV TOF-SIMS setup was demonstrated by imaging the chemical composition of CaCo-2 cells