Calidad óptica del ojo en sujetos fáquicos y seudofáquicos : método de doble paso / Manuel Redondo Manuel ; Directores, Pablo Artal Soriano, Jaime Miralles de Imperial Mora Figueroa.

Tesis-Universidad de Murcia.MEDICINA ESPINARDO. DEPOSITO. MU-Tesis 693.Consulte la tesis en: BCA. GENERAL. DEPOSITO. T.M-2087

Descriptores cuantitativos de la topografía corneal / Mª Dolores Romero Caballero ; directores Jaime Miralles de Imperial Mora-Figueroa, Angel Ramón Gutiérrez Ortega.

Tesis-Universidad de Murcia.MEDICINA ESPINARDO. DEPOSITO. MU-Tesis 544.Consulte la tesis en: BCA. GENERAL. ARCHIVO UNIVERSITARIO. T.M.-1618

Estudio de la microglía en la retina de "carassius auratus" : efecto de la axotomía sobre las células microgliales / Mercedes Salvador Silva ; directores Mª Paz Villegas Pérez, Jaime Miralles de Imperial Mora-Figueroa, Manuel Vidal Sanz.

Tesis-Universidad de Murcia.MEDICINA ESPINARDO. DEPOSITO. MU-Tesis 561.Consulte la tesis en: BCA. GENERAL. ARCHIVO UNIVERSITARIO. T.M.-1720

Estudio de la presión intraocular y del glaucoma en una población de la Región de Murcia / Juan José Zafra Pérez ; Directores Jaime Miralles de Imperial Mora-Figueroa, Mª Paz Villegas Pérez, Manuel Canteras Jordana.

Tesis - Universidad de Murcia.MEDICINA ESPINARDO. DEPOSITO. MU-Tesis 687.Consulte la tesis en: BCA. GENERAL. DEPOSITO. T.M-2098

Changes in the inner and outer retinal layers after acute increase of the intraocular pressure in adult albino Swiss mice

In adult albino mice the effects of increased intraocular pressure on the outer retina and its circuitry was investigated at intervals ranging 3–14 weeks. Ocular hypertension (OHT) was induced by cauterizing the vessels draining the anterior part of the mice eye, as recently reported (Salinas-Navarro et al., 2009a). Electroretinographic (ERG) responses were recorded simultaneously from both eyes and compared each other prior to and at different survival intervals of 2, 8 or 12 weeks after lasering. Animals were processed at 3, 9 or 14 weeks after lasering, and radial sections were obtained in the cryostat and further processed for immunocytochemistry using antibodies against recoverin, γ-transducin, Protein Kinase C-α (PKC-α), calbindin or synaptophysin. The synaptic ribbons were identified using an antibody against the protein bassoon, which labels photoreceptor ribbons and nuclei were identified using TO-PRO. Laser photocoagulation of the perilimbar and episcleral veins of the left eye resulted in an increase in mean intraocular pressure to approximately over twice its baseline by 24 h that was maintained for approximately five days reaching basal levels by 1 week. ERG recordings from the different groups of mice showed their a-, b-wave and scotopic threshold response (STR) amplitudes, when compared to their contralateral fellow eye, reduced to 62%, 52% and 23% at 12 weeks after lasering. Three weeks after lasering, immunostaining with recoverin and transducin antibodies could not document any changes in the outer nuclear layer (ONL) but both ON-rod bipolar and horizontal cells had lost their dendritic processes in the outer plexiform layer (OPL). Sprouting of horizontal and bipolar cell processes were observed into the ONL. Fourteen weeks after lasering, protein kinase-C antibodies showed morphologic changes of ON-rod bipolar cells and calbindin staining showed abnormal horizontal cells and a loss of their relationship with their presynaptic input. Moreover, at this time, quantitative studies indicate significant diminutions in the number of photoreceptor synaptic ribbons/100 μm, and in the thickness of the outer nuclear and plexiform layer, when compared to their fellow eyes. Increased intraocular pressure in Swiss mice results in permanent alterations of their full field ERG responses and in changes of the inner and outer retinal circuitries.This work was supported by research grants from the Regional Government of Murcia, Spanish Ministry of Science and Innovation and Fundaluce; FIS PIO06/0780 (MPVP); 04446/GERM/07, SAF 2009-10385; RD07/0062/0001 (MVS), FIS PI04/2399 (IP), BFU2009-07793/BFI, RD07/0062/0012, Fundaluce, ONCE (NC), RD07/0062/0008 (PdlV)