Efecto de la frecuencia de inmersión y la disponibilidad de medio de cultivo en la multiplicación del cultivar híbrido FHIA-21 (Musa AAAB) en sistemas de inmersión temporal

The present work had as objectives to study the effect of the immersion frequency and the availability of culture medium per shoot in the multiplication of the cultivar hybrid FHIA-21 using temporary immersion systems. It was demonstrated that it is possible to multiply this plant material in systems with culture flasks of two different capacities, obtaining superior results than in semisolid culture medium. A multiplication rate of 4.95 was achieved with flasks of 1.0 litre of capacity after 25 days of culture with four immersions a day, while the best results with temporary immersion systems of 10 litres of capacity were also obtained with four immersions a day, but using 50 ml of culture medium per shoot, reaching a total of 370 shoots by temporary immersion systems and a multiplication rate of 6.16. It was proven that the shoot of cultivar hybrid FHIA-21 could be multiplied during several successive subcultures in these systems and the presence of hiperhydrated shoot was not observed.Key words: automation, banana, liquid culture mediumEl presente trabajo tuvo como objetivos estudiar el efecto de la frecuencia de inmersión y la disponibilidad de medio de cultivo por brote en la multiplicación del cultivar híbrido FHIA-21 con el uso de sistemas de inmersión temporal. Se demostró que es posible multiplicar este material vegetal en sistemas con frascos de cultivo de dos capacidades diferentes y que los resultados que se pueden obtener son superiores a los obtenidos en medio de cultivo semisólido. Con frascos de 1.0 litro de capacidad se logró un coeficiente de multiplicación de 4.95 con cuatro inmersiones al día después de 25 días de cultivo, mientras que los mejores resultados con sistemas de inmersión temporal de 10 litros de capacidad se lograron también con cuatro inmersiones al día, pero con 50 ml de medio de cultivo por brote, con lo cual se alcanzó un total de 370 brotes por sistema de inmersión temporal y un coeficiente de multiplicación de 6.16. Se comprobó que los brotes del cultivar híbrido FHIA-21 puedieron ser multiplicados durante varios subcultivos sucesivos en estos sistemas y no se observó la presencia de brotes hiperhidratados.Palabras clave: automatización, medio de cultivo líquido, plátan

Effect of the immersion frequency and the availability of culture medium in the multiplication of the hybrid cultivar FHIA-21 (Musa AAAB) in temporary immersion systems

The present work had as objectives to study the effect of the immersion frequency and the availability of culture medium per shoot in the multiplication of the cultivar hybrid FHIA-21 using temporary immersion systems. It was demonstrated that it is possible to multiply this plant material in systems with culture flasks of two different capacities, obtaining superior results than in semisolid culture medium. A multiplication rate of 4.95 was achieved with flasks of 1.0 litre of capacity after 25 days of culture with four immersions a day, while the best results with temporary immersion systems of 10 litres of capacity were also obtained with four immersions a day, but using 50 ml of culture medium per shoot, reaching a total of 370 shoots by temporary immersion systems and a multiplication rate of 6.16. It was proven that the shoot of cultivar hybrid FHIA-21 could be multiplied during several successive subcultures in these systems and the presence of hiperhydrated shoot was not observed. Key words: automation, banana, liquid culture mediu