Time and magnitude of responses were estimated from the time course microarray data using 1

5-fold expression changes as a threshold (C), and then used as coordinates in a scatter-plot. Tentative target genes were identified as shown in Fig 1.<p><b>Copyright information:</b></p><p>Taken from "Identification of , , and downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation data"</p><p>http://www.biomedcentral.com/1471-2164/9/269</p><p>BMC Genomics 2008;9():269-269.</p><p>Published online 3 Jun 2008</p><p>PMCID:PMC2424064.</p><p></p

Examples of tentative target genes responding differentially to the suppression of , , and

<p><b>Copyright information:</b></p><p>Taken from "Identification of , , and downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation data"</p><p>http://www.biomedcentral.com/1471-2164/9/269</p><p>BMC Genomics 2008;9():269-269.</p><p>Published online 3 Jun 2008</p><p>PMCID:PMC2424064.</p><p></p

Effects of aging and calorie restriction on the global gene expression profiles of mouse testis and ovary-2

or testis by at least two-fold. Functional categories of genes were assembled from GO annotations and from PubMed.<p><b>Copyright information:</b></p><p>Taken from "Effects of aging and calorie restriction on the global gene expression profiles of mouse testis and ovary"</p><p>http://www.biomedcentral.com/1741-7007/6/24</p><p>BMC Biology 2008;6():24-24.</p><p>Published online 3 Jun 2008</p><p>PMCID:PMC2426674.</p><p></p

Effects of aging and calorie restriction on the global gene expression profiles of mouse testis and ovary-3

Hs in ovary and testis, respectively. (C), (D) Proportion of genes that were over-expressed either in ovary or testis, respectively, by at least two-fold among genes that were increased or decreased by CR at specific age from 6 to 24 months.<p><b>Copyright information:</b></p><p>Taken from "Effects of aging and calorie restriction on the global gene expression profiles of mouse testis and ovary"</p><p>http://www.biomedcentral.com/1741-7007/6/24</p><p>BMC Biology 2008;6():24-24.</p><p>Published online 3 Jun 2008</p><p>PMCID:PMC2426674.</p><p></p

Effects of aging and calorie restriction on the global gene expression profiles of mouse testis and ovary-4

Nes in ovary; (C) three-dimensional heatmap of expression of the same genes in ovary; (D) experimental design for males; (E) two-dimensional heatmap of expression of 3000 most significant genes in testis (819 genes overlapped with ovary); (F) three-dimensional heatmap of expression of the same genes in testis.<p><b>Copyright information:</b></p><p>Taken from "Effects of aging and calorie restriction on the global gene expression profiles of mouse testis and ovary"</p><p>http://www.biomedcentral.com/1741-7007/6/24</p><p>BMC Biology 2008;6():24-24.</p><p>Published online 3 Jun 2008</p><p>PMCID:PMC2426674.</p><p></p

Effects of aging and calorie restriction on the global gene expression profiles of mouse testis and ovary-1

or testis by at least two-fold. Functional categories of genes were assembled from GO annotations and from PubMed.<p><b>Copyright information:</b></p><p>Taken from "Effects of aging and calorie restriction on the global gene expression profiles of mouse testis and ovary"</p><p>http://www.biomedcentral.com/1741-7007/6/24</p><p>BMC Biology 2008;6():24-24.</p><p>Published online 3 Jun 2008</p><p>PMCID:PMC2426674.</p><p></p

Expression and endothelial-like and hepatocyte-like differentiation for mMAPC-1, mMAPC-2, mClone-3, rMAPC, and rClone-2

The levels of () mRNA in mouse (m, left) and rat (r, right) clones compared with those of mRNA. The mouse clones are also compared with mESCs. ΔCT is difference in threshold cycles calculated as Oct4 CT - Gapdh CT. ND, not detected. Endothelial-like differentiation. mRNA levels of endothelial markers in mouse (left panel) and rat (right panel) clones before and after differentiation, measured at day 9 in two independent differentiations of each clone. Levels are compared with those in universal mouse RNA and rat spleen RNA, respectively. Left panel: blue diamonds, Pecam (×10) (values shown were scaled by the factors in brackets); pink squares, Lyve1; orange triangles, vWF. Right panel: blue diamonds, VE-Cad (×100); pink squares, Flt-1; orange triangles, Flk-1; turquoise crosses, vWF (×100). Hepatocyte-like differentiation. mRNA levels of hepatocyte markers in mouse and rat clones before and after differentiation. Levels are compared with levels in mouse hepatocytes and rat liver, respectively. Two representative differentiations measured at day 18 are shown. Left panel: blue diamonds, F2 (×100); pink squares, Tat, (×10); green triangles, Afp (×10); turquoise crosses, Ttr (×10). Right panel: blue diamonds, Afp (×100); pink squares, Alb, (×10); orange triangles, Tat (×100); turquoise crosses, Ttr (×10). See text for abbreviations.<p><b>Copyright information:</b></p><p>Taken from "Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity"</p><p>http://genomebiology.com/2007/8/8/R163</p><p>Genome Biology 2007;8(8):R163-R163.</p><p>Published online 6 Aug 2007</p><p>PMCID:PMC2374994.</p><p></p

Cell-surface phenotype and Oct4 protein expression of mouse and rat clones evaluated by flow cytometry

Flow cytometry results for mouse clones. From left to right: MSCs; mMAPC-1; mMAPC-2; mClone-3; and MSCs cultured in MAPC conditions. The histogram in each panel plots the number of cells (as a percentage of the maximum) on the vertical axis against the fluorescence intensity of the labeled antibody bound to the indicated protein (horizontal axis). The horizontal line on each histogram indicates the fluorescence range that contains the indicated percentage of cells positive for that protein. Flow cytometry results for the rat clones rMAPC-1 and rClone-2.<p><b>Copyright information:</b></p><p>Taken from "Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity"</p><p>http://genomebiology.com/2007/8/8/R163</p><p>Genome Biology 2007;8(8):R163-R163.</p><p>Published online 6 Aug 2007</p><p>PMCID:PMC2374994.</p><p></p

Genes for proteins localized to the extracellular space and for transcriptional regulators clustered according to expression profiles in MAPC/ESC/NS comparison

Genes expressed at levels at least twofold higher in both MAPCs and ESCs than in NS (k-means clusters 2 and 4). Genes expressed in MAPCs at levels at least twofold higher than in both ESCs and NS (k-means cluster 3). The yellow bars indicate genes whose products are localized to the extracellular space; the blue bars indicate genes for transcriptional regulators. Red and green indicate higher and lower levels of expression, respectively.<p><b>Copyright information:</b></p><p>Taken from "Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity"</p><p>http://genomebiology.com/2007/8/8/R163</p><p>Genome Biology 2007;8(8):R163-R163.</p><p>Published online 6 Aug 2007</p><p>PMCID:PMC2374994.</p><p></p

PCA and NMF analysis of the gene-expression data of mouse MSC, MAPC-1, MAPC-2 and ESC

Percentage variation captured in each principal component (PC). PCs are ordered from 1 to 4 according to the percentage of the total variance they capture. Samples plotted in the first two components' space. Distance of samples in the component space is indicative of similarity in expression profiles. Consensus matrix from NMF. Model selection in NMF is based on a consensus matrix that contains the probability that a pair of samples is assigned to the same group. Probability values correspond to the colors in the key. M-1, MAPC-1; M-2, MAPC-2; Metagene profiles from NMF plotted as logarithm of the probe set intensity.<p><b>Copyright information:</b></p><p>Taken from "Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity"</p><p>http://genomebiology.com/2007/8/8/R163</p><p>Genome Biology 2007;8(8):R163-R163.</p><p>Published online 6 Aug 2007</p><p>PMCID:PMC2374994.</p><p></p