Temporal and Spatial Impact of Human Cadaver Decomposition on Soil Bacterial and Arthropod Community Structure and Function

As vertebrate carrion decomposes, there is a release of nutrient-rich fluids into theunderlying soil, which can impact associated biological community structure andfunction. How these changes alter soil biogeochemical cycles is relatively unknown and may prove useful in the identification of carrion decomposition islands that have long lasting, focal ecological effects. This study investigated the spatial (0, 1, and 5 m) and temporal (3–732 days) dynamics of human cadaver decomposition on soil bacterial and arthropod community structure and microbial function. We observed strong evidence of a predictable response to cadaver decomposition that varies over space for soil bacterial and arthropod community structure, carbon (C) mineralization and microbial substrate utilization patterns. In the presence of a cadaver (i.e., 0 m samples), the relative abundance of Bacteroidetes and Firmicutes was greater, while the relative abundance of Acidobacteria, Chloroflexi, Gemmatimonadetes, and Verrucomicrobia was lower when compared to samples at 1 and 5 m. Micro-arthropods were more abundant (15 to 17-fold) in soils collected at 0 m compared to either 1 or 5 m, but overall, micro-arthropod community composition was unrelated to either bacterial community composition or function. Bacterial community structure and microbial function also exhibited temporal relationships, whereas arthropod community structure did not. Cumulative precipitation was more effective in predicting temporal variations in bacterial abundance and microbial activity than accumulated degree days. In the presence of the cadaver (i.e., 0 m samples), the relative abundance of Actinobacteria increased significantly with cumulative precipitation. Furthermore, soil bacterial communities and C mineralization were sensitive to the introduction of human cadavers as they diverged from baseline levels and did not recover completely in approximately 2 years. These data are valuable for understanding ecosystem function surrounding carrion decomposition islands and can be applicable to environmental bio-monitoring and forensic sciences

Prolonged exposure to manure from livestock administered antibiotics decreases ecosystem carbon-use efficiency and alters nitrogen cycling

Microbial communities drive soil ecosystem function but are also susceptible to environmental disturbances. We investigated whether exposure to manure sourced from cattle either administered or not administered antibiotics affected microbially-mediated terrestrial ecosystem function. We quantified changes in microbial community composition, and terrestrial elemental cycling via a stable isotope pulse-chase. Exposure to manure from antibiotic-treated cattle caused: i) changes in microbial community structure; and ii) alterations in elemental cycling throughout the terrestrial system. This exposure caused changes in fungal:bacterial, as well as changes in bacterial community structure. Additionally, exposure to manure from cattle treated with pirlimycin resulted in an approximate two-fold increase in ecosystem respiration of recently fixed-carbon, and a greater proportion of recently-added nitrogen in plant and soil pools compared to the control manure. Manure from antibiotic-treated cattle therefore affects terrestrial ecosystem function via the soil microbiome, causing decreased ecosystem carbon use efficiency, and altered nitrogen cycling

Effects of Spatial Variability and Drainage on Extracellular Enzyme Activity in Coastal Freshwater Forested Wetlands of Eastern North Carolina, USA

Drainage of freshwater wetlands is common in coastal regions, although the effects on microbial extracellular enzyme activity (a key mediator of soil organic matter decomposition) in relation to spatial variability (microtopography and soil depth) are poorly understood. Soils were collected from organic (Oi, Oe, Oa) and mineral (A, AB, B) horizons from a natural and drained coastal forested wetland in North Carolina, USA. Activity of seven enzymes were measured: α-glucosidase (AG), β-glucosidase (BG), cellobiohydrolase (CBH), xylosidase (XYL), phenol oxidase (POX), peroxidase (PER) and N-acetyl glucosamide (NAG). Enzyme activity rates were normalized by soil weight, soil organic C (SOC), and microbial biomass C (MBC). Specific enzyme activity (per SOC or MBC) was more sensitive to drainage and soil depth compared to normalization by soil weight. In Oi and Oa horizons, specific enzyme activity (per MBC) (AG, BG, XYL, POX, PER) was higher in the natural compared to drained wetland but lower (AG, CBH, XYL, POX, PER, NAG) in the AB or B mineral soils. Results from this study indicate that organic soil horizons of natural freshwater wetlands contain a highly active microbial community driven by inputs of plant-derived C, while deeper soils of the drained wetland exhibit higher microbial metabolic activity, which likely plays a role in SOC storage of these systems

Effects of Spatial Variability and Drainage on Extracellular Enzyme Activity in Coastal Freshwater Forested Wetlands of Eastern North Carolina, USA

Drainage of freshwater wetlands is common in coastal regions, although the effects on microbial extracellular enzyme activity (a key mediator of soil organic matter decomposition) in relation to spatial variability (microtopography and soil depth) are poorly understood. Soils were collected from organic (Oi, Oe, Oa) and mineral (A, AB, B) horizons from a natural and drained coastal forested wetland in North Carolina, USA. Activity of seven enzymes were measured: α-glucosidase (AG), β-glucosidase (BG), cellobiohydrolase (CBH), xylosidase (XYL), phenol oxidase (POX), peroxidase (PER) and N-acetyl glucosamide (NAG). Enzyme activity rates were normalized by soil weight, soil organic C (SOC), and microbial biomass C (MBC). Specific enzyme activity (per SOC or MBC) was more sensitive to drainage and soil depth compared to normalization by soil weight. In Oi and Oa horizons, specific enzyme activity (per MBC) (AG, BG, XYL, POX, PER) was higher in the natural compared to drained wetland but lower (AG, CBH, XYL, POX, PER, NAG) in the AB or B mineral soils. Results from this study indicate that organic soil horizons of natural freshwater wetlands contain a highly active microbial community driven by inputs of plant-derived C, while deeper soils of the drained wetland exhibit higher microbial metabolic activity, which likely plays a role in SOC storage of these systems

Millennial-Scale Carbon Storage in Natural Pine Forests of the North Carolina Lower Coastal Plain: Effects of Artificial Drainage in a Time of Rapid Sea Level Rise

Coastal forested wetlands provide important ecosystem services along the southeastern region of the United States, but are threatened by anthropogenic and natural disturbances. Here, we examined the species composition, mortality, aboveground biomass, and carbon content of vegetation and soils in natural pine forests of the lower coastal plain in eastern North Carolina, USA. We compared a forest clearly in decline (termed “ghost forest”) adjacent to a roadside canal that had been installed as drainage for a road next to an adjacent forest subject to “natural” hydrology, unaltered by human modification (termed “healthy forest”). We also assessed how soil organic carbon (SOC) accumulation changed over time using 14C radiocarbon dating of wood sampled at different depths within the peat profile. Our results showed that the ghost forest had a higher tree density at 687 trees ha−1, and was dominated by swamp bays (Persea palustric), compared to the healthy forest, which had 265 trees ha−1 dominated by pond pine (Pinus serotina Michx). Overstory tree mortality of the ghost forest was nearly ten times greater than the healthy forest (p < 0.05), which actually contributed to higher total aboveground biomass (55.9 ± 12.6 Mg C ha−1 vs. 27.9 ± 8.7 Mg ha−1 in healthy forest), as the dead standing tree biomass (snags) added to that of an encroaching woody shrub layer during ecosystem transition. Therefore, the total aboveground C content of the ghost forest, 33.98 ± 14.8 Mg C ha−1, was higher than the healthy forest, 24.7 ± 5.2 Mg C ha−1 (p < 0.05). The total SOC stock down to a 2.3 m depth in the ghost forest was 824.1 ± 46.2 Mg C ha−1, while that of the healthy forest was 749.0 ± 170.5 Mg C ha−1 (p > 0.05). Carbon dating of organic sediments indicated that, as the sample age approaches modern times (surface layer year 2015), the organic soil accumulation rate (1.11 to 1.13 mm year−1) is unable to keep pace with the estimated rate of recent sea level rise (2.1 to 2.4 mm year−1), suggesting a causative relationship with the ecosystem transition occurring at the site. Increasing hydrologic stress over recent decades appears to have been a major driver of ecosystem transition, that is, ghost forest formation and woody shrub encroachment, as indicated by the far higher overstory tree mortality adjacent to the drainage ditch, which allows the inland propagation of hydrologic/salinity forcing due to SLR and extreme storms. Our study documents C accumulation in a coastal wetland over the past two millennia, which is now threatened due to the recent increase in the rate of SLR exceeding the natural peat accumulation rate, causing an ecosystem transition with unknown consequences for the stored C; however, much of it will eventually be returned to the atmosphere. More studies are needed to determine the causes and consequences of coastal ecosystem transition to inform the modeling of future coastal wetland responses to environmental change and the estimation of regional terrestrial C stocks and flux

Millennial-Scale Carbon Storage in Natural Pine Forests of the North Carolina Lower Coastal Plain: Effects of Artificial Drainage in a Time of Rapid Sea Level Rise

Coastal forested wetlands provide important ecosystem services along the southeastern region of the United States, but are threatened by anthropogenic and natural disturbances. Here, we examined the species composition, mortality, aboveground biomass, and carbon content of vegetation and soils in natural pine forests of the lower coastal plain in eastern North Carolina, USA. We compared a forest clearly in decline (termed “ghost forest”) adjacent to a roadside canal that had been installed as drainage for a road next to an adjacent forest subject to “natural” hydrology, unaltered by human modification (termed “healthy forest”). We also assessed how soil organic carbon (SOC) accumulation changed over time using 14C radiocarbon dating of wood sampled at different depths within the peat profile. Our results showed that the ghost forest had a higher tree density at 687 trees ha−1, and was dominated by swamp bays (Persea palustric), compared to the healthy forest, which had 265 trees ha−1 dominated by pond pine (Pinus serotina Michx). Overstory tree mortality of the ghost forest was nearly ten times greater than the healthy forest (p −1 vs. 27.9 ± 8.7 Mg ha−1 in healthy forest), as the dead standing tree biomass (snags) added to that of an encroaching woody shrub layer during ecosystem transition. Therefore, the total aboveground C content of the ghost forest, 33.98 ± 14.8 Mg C ha−1, was higher than the healthy forest, 24.7 ± 5.2 Mg C ha−1 (p −1, while that of the healthy forest was 749.0 ± 170.5 Mg C ha−1 (p > 0.05). Carbon dating of organic sediments indicated that, as the sample age approaches modern times (surface layer year 2015), the organic soil accumulation rate (1.11 to 1.13 mm year−1) is unable to keep pace with the estimated rate of recent sea level rise (2.1 to 2.4 mm year−1), suggesting a causative relationship with the ecosystem transition occurring at the site. Increasing hydrologic stress over recent decades appears to have been a major driver of ecosystem transition, that is, ghost forest formation and woody shrub encroachment, as indicated by the far higher overstory tree mortality adjacent to the drainage ditch, which allows the inland propagation of hydrologic/salinity forcing due to SLR and extreme storms. Our study documents C accumulation in a coastal wetland over the past two millennia, which is now threatened due to the recent increase in the rate of SLR exceeding the natural peat accumulation rate, causing an ecosystem transition with unknown consequences for the stored C; however, much of it will eventually be returned to the atmosphere. More studies are needed to determine the causes and consequences of coastal ecosystem transition to inform the modeling of future coastal wetland responses to environmental change and the estimation of regional terrestrial C stocks and flux

Environmental and Plant-Derived Controls on the Seasonality and Partitioning of Soil Respiration in an American Sycamore (<i>Platanus occidentalis)</i> Bioenergy Plantation Grown at Different Planting Densities

Bioenergy is one of the most considered alternatives to fossil fuels. Short-rotation woody crops (SRWCs) as bioenergy sources are capable of alleviating energy constraints and sequestering atmospheric CO2. However, studies investigating soil carbon (C) dynamics at SWRC plantations are scarce. We studied American sycamore (Platanus occidentalis) as a model tree species for SRWC at different planting densities ((1) 0.5 × 2.0 m (10,000 trees·ha−1 or tph), (2) 1.0 × 2.0 m (5000 tph), and (3) 2.0 × 2.0 m (2500 tph)) to examine seasonal variation in total soil respiration (Rtotal), partitioned into heterotrophic (Rh) and autotrophic (Ra) respiration, and we evaluated climatic and biological controls on soil respiration. Rtotal and Rh exhibited larger seasonal variation than Ra (p total was 0.60 ± 0.21 g·C·m−2·day−1 in winter and 1.41 ± 0.73 g·C·m−2·day−1 in fall. During the growing season, Rtotal was 2–7 times higher in spring (3.49 ± 1.44 g·C·m−2·day−1) and summer (4.01 ± 1.17 g·C·m−2·day−1) than winter. Average Rtotal was 2.30 ± 0.63 g·C·m−2·day−1 in 2500 tph, 2.43 ± 0.64 g·C·m−2·day−1 in 5000 tph, and 2.41 ± 0.75 g·C·m−2·day−1 in 10,000 tph treatments. Average Rh was 1.72 ± 0.40 g·C·m−2·day−1 in 2500 tph, 1.57 ± 0.39 g·C·m−2·day−1 in 5000 tph, and 1.93 ± 0.64 g·C·m−2·day−1 in 10,000 tph, whereas Ra had the lowest rates, with 0.59 ± 0.53 g·C·m−2·day−1 in 2500 tph, 0.86 ± 0.51 g·C·m−2·d−1 in 5000 tph, and 0.48 ± 0.34 g·C·m−2·day−1 in 10,000 tph treatments. Rh had a greater contribution to Rtotal (63%–80%) compared to Ra (20%–37%). Soil temperature was highly correlated to Rtotal (R2 = 0.92) and Rh (R2 = 0.77), while the correlation to Ra was weak (R2 = 0.21). Rtotal, Rh, and Ra significantly declined with soil water content extremes (e.g., 50%). Total root biomass in winter (469 ± 127 g·C·m−2) was smaller than in summer (616 ± 161 g·C·m−2), and the relationship of total root biomass to Rtotal, Rh, and Ra was only significant during the growing seasons (R2 = 0.12 to 0.50). The litterfall in 5000 tph (121 ± 16 g DW·m−2) did not differ (p > 0.05) from the 2500 tph (108 ± 16 g DW·m−2) or 10,000 tph (132 ± 16 g DW·m−2) treatments. In no circumstances were Rtotal, Rh, and Ra significantly correlated with litterfall amount across planting densities and seasons (p > 0.05). Overall, our results show that Rtotal in American sycamore SRWC is dominated by the heterotrophic component (Rh), is strongly correlated to soil environmental conditions, and can be minimized by planting at a certain tree density (5000 tph)

Ecosystem Productivity and Evapotranspiration Are Tightly Coupled in Loblolly Pine (Pinus taeda L.) Plantations along the Coastal Plain of the Southeastern U.S.

Forest water use efficiency (WUE), the ratio of gross primary productivity (GPP) to evapotranspiration (ET), is an important variable to understand the coupling between water and carbon cycles, and to assess resource use, ecosystem resilience, and commodity production. Here, we determined WUE for managed loblolly pine plantations over the course of a rotation on the coastal plain of North Carolina in the eastern U.S. We found that the forest annual GPP, ET, and WUE increased until age ten, which stabilized thereafter. WUE varied annually (2–44%), being higher at young plantation (YP, 3.12 ± 1.20 g C kg−1 H2O d−1) compared to a mature plantation (MP, 2.92 ± 0.45 g C kg−1 H2O d−1), with no distinct seasonal patterns. Stand age was strongly correlated with ET (R2 = 0.71) and GPP (R2 = 0.64). ET and GPP were tightly coupled (R2 = 0.86). Radiation and air temperature significantly affected GPP and ET (R2 = 0.71 − R2 = 0.82) at a monthly scale, but not WUE. Drought affected WUE (R2 = 0.35) more than ET (R2 = 0.25) or GPP (R2 = 0.07). A drought enhanced GPP in MP (19%) and YP (11%), but reduced ET 7% and 19% in MP and YP, respectively, resulting in a higher WUE (27–32%). Minor seasonal and interannual variation in forest WUE of MP (age &gt; 10) suggested that forest functioning became stable as stands matured. We conclude that carbon and water cycles in loblolly pine plantations are tightly coupled, with different characteristics in different ages and hydrologic regimes. A stable WUE suggests that the pine ecosystem productivity can be readily predicted from ET and vice versa. The tradeoffs between water and carbon cycling should be recognized in forest management to achieve multiple ecosystem services (i.e., water supply and carbon sequestration)

Effects of microtopography on absorptive and transport fine root biomass, necromass, production, mortality and decomposition in a coastal freshwater forested wetland, southeastern USA

International audienceForested wetlands are an important carbon (C) sink. Fine roots (diameter < 2 mm) dominate belowground C cycling and can be functionally defined into absorptive roots (order 1–2) and transport roots (order ≥ 3). However, effects of microtopography on the function-based fine root dynamics in forested wetlands are poorly understood. We studied fine root biomass allocation and biomass, necromass, mass loss rate, production, mortality and decomposition of absorptive and transport roots in hummocks and hollows in a coastal plain freshwater forested wetland (FFW) in the southeastern USA using dynamic-flow method. Biomass ratios of first- to second-order roots and absorptive to transport roots and the biomass and necromass of absorptive and transport roots were significantly higher in top 0–10 cm organic peat layer than in 10–20 cm muck and mineral layer, and were significantly higher in hummocks than in hollows. The mass loss rate, production, mortality and decomposition were significantly higher in hummocks than in hollows. Absorptive roots did not have a lower mass loss rate than transport roots. Microtopography significantly affected the contributions of absorptive and transport roots to the total production, mortality and decomposition. Production, mortality and decomposition of absorptive roots were higher than those of transport roots in hummocks but lower than those of transport roots in hollows. Total (hummocks plus hollows) fine root production, mortality and decomposition were 455 ± 106 g m−2 y−1, 475 ± 79 g m−2 y−1 and 392 ± 60 g m−2 y−1, respectively. Greater mortality than decomposition resulted in net fine root C input to soil. The observed microtopographic controls on fine root dynamics have great implications for soil C cycling. As sea level rises, the relative area of hollows in coastal plain FFWs will increase, causing a decrease in fine root mass loss rate, biomass, production, mortality and decomposition and it is the balance of these processes that will determine future soil C storage and cycling