Aptamer-based single-molecule imaging of insulin receptors in living cells

We present a single-molecule imaging platform that quantitatively explores the spatiotemporal dynamics of individual insulin receptors in living cells. Modified DNA aptamers that specifically recognize insulin receptors (IRs) with a high affinity were selected through the SELEX process. Using quantum dot-labeled aptamers, we successfully imaged and analyzed the diffusive motions of individual IRs in the plasma membranes of a variety of cell lines (HIR, HEK293, HepG2). We further explored the cholesterol-dependent movement of IRs to address whether cholesterol depletion interferes with IRs and found that cholesterol depletion of the plasma membrane by methyl-??-cyclodextrin reduces the mobility of IRs. The aptamer-based single-molecule imaging of IRs will provide better understanding of insulin signal transduction through the dynamics study of IRs in the plasma membrane.open1

Single-molecule Force-Fluorescense Spectroscopy in Expanded Focal Depth

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Too much at stake to uphold sport integrity? High-performance athletes’ involvement in match-fixing

Non-betting-related match-fixing constitutes an important integrity issue in contemporary sports. With varied forms ranging from passive tanking to the purposeful, coercive, calculated bribing of others to gain advantages, non-betting-related match-fixing can be a form of corruption that deters sport development. This paper examines high-performance athletes’ involvement in non-betting-related match-fixing in South Korea. Drawing from survey data (n=731), this paper describes and analyses the prevalence of match-fixing, its locales (i.e., levels of competition) and origins (i.e., who made the offer/approach). Results show that: (1) 74 athletes (10.12%) were approached to take part in match-fixing, while 33 of those athletes (4.51%) actually participated; and (2) the match-fixing offers were usually made ‘by coaches’, ‘at high school-level nationwide competitions’, ‘for the purpose of entering universities’. Finally, this paper concludes by suggesting that the excessive incentives (e.g., university admission) linked with elite sport development structures may account for the strong motive behind non-betting-related match-fixing, and its endangering of sport integrity.</p

Globalization, migration, citizenship, and sport celebrity: Locating Lydia Ko between and beyond New Zealand and South Korea

The contested terrain surrounding globalization, migration, citizenship, and national identity shape the context in which modern sport celebrity develops in Asia. Focusing on female golf phenomenon Lydia Ko, the analysis locates her celebrity and national identity between her place of birth–Korea–and her place of citizenship–New Zealand. Several intersecting factors influenced Ko’s celebrity and identity construction including changes in New Zealand immigration policy, changes in Korean state policy towards overseas nationals, negatively viewed attitudes and behaviours of previous foreign-born celebrities of Korean-descent, and Ko’s own public proclamations regarding her national identity

Extended depth of field for single biomolecule optical imaging-force spectroscopy

Real-time optical imaging combined with single-molecule manipulation broadens the horizons for acquiring information about the spatiotemporal localization and the mechanical details of target molecules. To obtain an optical signal outside the focal plane without unintended interruption of the force signal in single-molecule optical imaging-force spectroscopy, we developed an optical method to extend the depth of field in a high numerical aperture objective (>= 1.2), required to visualize a single fluorophore. By axial scanning, using an electrically tunable lens with a fixed sample, we were successfully able to visualize the epidermal growth factor receptor (EGFR) moving along the three-dimensionally elongated filamentous actin bundles connecting cells (intercellular nanotube), while another EGFR on the intercellular nanotube was trapped by optical tweezers in living cells. Our approach is simple, fast and inexpensive, but it is powerful for imaging target molecules axially in singlemolecule optical imaging-force spectroscopy. (C) 2017 Optical Society of America under the terms of the OSA Open Access Publishing Agreement11Nsciescopu

Odds-wise view: Whose ideas prevail in the global integrity campaigns against match-fixing?

The global expansion of sports betting has resulted in the formation of (inter)national governing regimes aimed at sustaining revenue and regulating attendant issues, including match-fixing. This article explores the workings of these regimes vis-à-vis the management of match-fixing issues in sport. More particularly, this article focuses on betting monitoring programmes as countermeasures against match-fixing and conceptualises these as social instruments that ultimately define issues and influence the wider integrity agenda of anti-match-fixing campaigns. Analysing documentary, observation and interview data from two disparate monitoring programmes, the results show that betting monitoring is a technical extension of corporate risk management, invariably reflecting the business interests of the betting industry. Therefore, the operating logic of betting monitoring defines match-fixing as an act of sabotaging the competitive edge of betting companies. Moreover, this interest-laden paradigm reigns within the broader policy agenda of sport integrity by equating the betting industry's interest with that of sport. From this, the article suggests that betting monitoring plays a part in the legitimation of commercial gambling by reframing the issue of match-fixing as a common enemy that gambling and sport join forces to combat, not a risk that gambling brings to sport

How the filopodia make intercellular nanotube: the effects of torque and restorative force

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The mechanism of formation of the intercellular nanotubes: Langevin dynamics simulation on the interwound filopodia

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