Dietary Probiotic Bacillus licheniformis TC22 Increases Growth, Immunity, and Disease Resistance, against Vibrio splendidus Infection in Juvenile Sea Cucumbers Apostichopus japonicus

In this study we examined the effects of probiotic Bacillus licheniformis TC22 on growth, immunity, and disease resistance against Vibrio splendidus in juvenile sea cucumbers Apostichopus japonicus. For 30 days, sea cucumbers were fed diets with TC22 at 0 (control), 105, 107, and 109 CFU/g respectively. Results showed that dietary TC22 at 109 CFU/g significantly improved (P0.05). Dietary TC22 at 109 CFU/g significantly improved phagocytosis, and total nitric oxide synthase activity in sea cucumbers (P0.05). Respiratory burst in sea cucumbers fed dietary TC22 at 109 CFU/g was significantly higher than those fed dietary TC22 at 107 CFU/g (P<0.05). Cumulative mortality after V. splendidus challenge decreased significantly in the sea cucumbers fed with TC22 at 109 CFU/g (P<0.05). The present study confirmed dietary B. licheniformis TC22 at 109 CFU/g could significantly improve immunity and disease resistance in juvenile A. japonicus

Higher fibrinogen and neutrophil-to-lymphocyte ratio are associated with the early poor response to intravenous thrombolysis in acute ischemic stroke

BackgroundInflammation and platelet activation play pivotal roles in acute ischemic stroke (AIS) pathogenesis. Early response to thrombolysis is a vital indicator for the long-term prognosis of AIS. However, the correlation between fibrinogen or the neutrophil-to-lymphocyte ratio (NLR) and the early response to intravenous thrombolysis in patients with AIS remains unclear.MethodsAIS patients undergoing intravenous thrombolysis were enrolled between January 2018 and May 2023. Blood cell counts were sampled before thrombolysis. A good response was defined as a National Institutes of Health Stroke Scale (NIHSS) score decreased ≥4 or complete recovery 24 h after thrombolysis treatment. A poor response was defined as any increase in the NIHSS score or a decrease in the NIHSS score &lt;4 at the 24 h after thrombolysis treatment compared with that at admission. Logistic regression analysis was performed to explore the relationship of the fibrinogen level and NLR with a poor thrombolysis response. Receiver operating characteristic (ROC) analysis was used to assess the ability of the fibrinogen level and NLR to discriminate poor responders.ResultsAmong 700 recruited patients, 268 (38.29%) were diagnosed with a good response, and 432 (61.71%) were diagnosed with a poor response to intravenous thrombolysis. A binary logistic regression model indicated that an elevated fibrinogen level (odds ratio [OR], 1.693; 95% confidence interval [CI] 1.325–2.122, P &lt; 0.001) and NLR (OR, 1.253; 95% CI, 1.210–2.005, P = 0.001) were independent factors for a poor response. The area under the curve (AUC) values for the fibrinogen level, NLR and fibrinogen level combined with the NLR for a poor response were 0.708, 0.605, and 0.728, respectively.ConclusionsOur research indicates that the levels of fibrinogen and NLR at admission can be used as a prognostic factor to predict early poor response to intravenous thrombolysis

Ghrelin enhances tubular magnesium absorption in the kidney

Osteoporosis after bariatric surgery is an increasing health concern as the rate of bariatric surgery has risen. In animal studies mimicking bariatric procedures, bone disease, together with decreased serum levels of Ca2+, Mg2+ and the gastric hormone Ghrelin were described. Ghrelin regulates metabolism by binding to and activating the growth hormone secretagogue receptor (GHSR) which is also expressed in the kidney. As calcium and magnesium are key components of bone, we tested the hypothesis that Ghrelin-deficiency contributes to osteoporosis via reduced upregulation of the renal calcium channel TRPV5 and the heteromeric magnesium channel TRPM6/7. We expressed GHSR with TRPV5 or TRPM6/7 channel in HEK293 cells and treated them with purified Ghrelin. Whole-cell current density was analyzed by patch-clamp recording. Nephron-specific gene expression was performed by tubular microdissection followed by qPCR in wild-type (WT) mice, and immunofluorescent imaging of GHSR-eGFP mice. Tubular magnesium homeostasis was analyzed in GHSR-null and WT mice at baseline and after caloric restriction. After Ghrelin exposure, whole-cell current density did not change for TRPV5 but increased for TRPM6/7 in a dose-dependent fashion. Applying the Ghrelin-mimetic (D-Trp7, Ala8,D-Phe10)-α-MSH (6–11) amide without and with the GHSR antagonist (D-Lys3)-GHRP6, we confirmed the stimulatory role of Ghrelin towards TRPM6/7. As GHSR initiates downstream signaling via protein kinase A (PKA), we found that the PKA inhibitor H89 abrogated TRPM6/7 stimulation by Ghrelin. Similarly, transfected Gαs, but not the Gαs mutant Q227L, nor Gαi2, Gαq, or Gα13 upregulated TRPM6/7 current density. In microdissected TALs and DCTs similar levels of GHSR mRNA were detected. In contrast, TRPM6 mRNA was expressed in the DCT and also detected in the TAL at 25% expression compared to DCT. Immunofluorescent studies using reporter GHSR-eGFP mice showed a strong eGFP signal in the TAL but surprisingly displayed no eGFP signal in the DCT. In 3-, 6-, and 9-month-old GHSR-null and WT mice, baseline serum magnesium was not significantly different, but 24-h urinary magnesium excretion was elevated in 9-month-old GHSR-null mice. In calorically restricted GHSR-null mice, we detected excess urinary magnesium excretion and reduced serum magnesium levels compared to WT mice. The kidneys from calorically restricted WT mice showed upregulated gene expression of magnesiotropic genes Hnf1b, Cldn-16, Cldn-19, Fxyd-2b, and Parvalbumin compared to GHSR-null mice. Our in vitro studies show that Ghrelin stimulates TRPM6/7 via GHSR and Gαs-PKA signaling. The murine studies are consistent with Ghrelin-GHSR signaling inducing reduced urinary magnesium excretion, particularly in calorically restricted mice when Ghrelin levels are elevated. This effect may be mediated by Ghrelin-upregulation of TRPM6 in the TAL and/or upregulation of other magnesiotropic genes. We postulate that rising Ghrelin levels with hunger contribute to increased renal Mg2+ reabsorption to compensate for lack of enteral Mg2+ uptake

Transcriptome Analysis of H2O2-Treated Wheat Seedlings Reveals a H2O2-Responsive Fatty Acid Desaturase Gene Participating in Powdery Mildew Resistance

Hydrogen peroxide (H2O2) plays important roles in plant biotic and abiotic stress responses. However, the effect of H2O2 stress on the bread wheat transcriptome is still lacking. To investigate the cellular and metabolic responses triggered by H2O2, we performed an mRNA tag analysis of wheat seedlings under 10 mM H2O2 treatment for 6 hour in one powdery mildew (PM) resistant (PmA) and two susceptible (Cha and Han) lines. In total, 6,156, 6,875 and 3,276 transcripts were found to be differentially expressed in PmA, Han and Cha respectively. Among them, 260 genes exhibited consistent expression patterns in all three wheat lines and may represent a subset of basal H2O2 responsive genes that were associated with cell defense, signal transduction, photosynthesis, carbohydrate metabolism, lipid metabolism, redox homeostasis, and transport. Among genes specific to PmA, ‘transport’ activity was significantly enriched in Gene Ontology analysis. MapMan classification showed that, while both up- and down- regulations were observed for auxin, abscisic acid, and brassinolides signaling genes, the jasmonic acid and ethylene signaling pathway genes were all up-regulated, suggesting H2O2-enhanced JA/Et functions in PmA. To further study whether any of these genes were involved in wheat PM response, 19 H2O2-responsive putative defense related genes were assayed in wheat seedlings infected with Blumeria graminis f. sp. tritici (Bgt). Eight of these genes were found to be co-regulated by H2O2 and Bgt, among which a fatty acid desaturase gene TaFAD was then confirmed by virus induced gene silencing (VIGS) to be required for the PM resistance. Together, our data presents the first global picture of the wheat transcriptome under H2O2 stress and uncovers potential links between H2O2 and Bgt responses, hence providing important candidate genes for the PM resistance in wheat

The physiological functionality of PGR5/PGRL1-dependent cyclic electron transport in sustaining photosynthesis

The cyclic electron transport (CET), after the linear electron transport (LET), is another important electron transport pathway during the light reactions of photosynthesis. The proton gradient regulation 5 (PGR5)/PRG5-like photosynthetic phenotype 1 (PGRL1) and the NADH dehydrogenase-like complex pathways are linked to the CET. Recently, the regulation of CET around photosystem I (PSI) has been recognized as crucial for photosynthesis and plant growth. Here, we summarized the main biochemical processes of the PGR5/PGRL1-dependent CET pathway and its physiological significance in protecting the photosystem II and PSI, ATP/NADPH ratio maintenance, and regulating the transitions between LET and CET in order to optimize photosynthesis when encountering unfavorable conditions. A better understanding of the PGR5/PGRL1- mediated CET during photosynthesis might provide novel strategies for improving crop yield in a world facing more extreme weather events with multiple stresses affecting the plants

A survey of subjective constipation in Parkinson’s disease patients in shanghai and literature review

Abstract Background Constipation is one of the most frequent non-motor symptoms (NMS) in Parkinson’s disease (PD) and the prevalence of constipation in PD patients varies among different studies. We designed this study to survey the prevalence and clinical characteristics of subjective constipation and the appearance chronology between the emergence of constipation and onset of motor symptoms in PD patients from Shanghai, China. Methods 268 PD patients were continuously recruited into this study. Parkinson’s related clinical information of the participants was collected. A spectrum of motor and nonmotor features was assessed with scales and questionnaires. Subjective constipation was defined by ROME III criteria. Results 54.10% PD patients suffer from constipation. Among them, there was 47.59% having constipation before onset of motor symptoms. Compared with patients without constipation, patients with constipation reported lower daily water intake and less exercise, and were dominated by bradykinetic-rigid motor phenotype at onset and were prone to have anxiety, depression and insomnia. The time span between constipation and the onset of motor symptoms was (6.62 ± 9.32) years. Constipation occurred more frequently between 2 and 10 years before onset of motor symptoms. Patients suffering with constipation were then divided into two groups according to the time sequence of constipation and motor onset: ‘constipation pre-motor sign’ group and ‘constipation post-motor sign’ group. Total timespan from earliest initial symptoms to present was similar. Compared with ‘constipation post-motor sign’ group, the patients in ‘constipation pre-motor sign’ group experienced an older motor symptoms onset age, less serious motor symptoms, more serious constipation and less daily levodopa dosage. Conclusions Our results supported that constipation could be a pre-motor symptom of PD. Different clinical characteristics were found in different constipation-loading time relative to motor symptoms. Research of constipation may be useful to better understand the early stages of PD and assessment of constipation with validated criteria may have utility as a risk factor for predicting PD in the prodromal phase

Multiple 3.8-3.1Ga tectono-magmatic events in a newly discovered area of ancient rocks (the Shengousi Complex), Anshan, North China Craton

Near Anshan city in the North China Craton, the oldest rocks in Asia (⩾3300 Ma) have been thoroughly documented at two localities (Baijiafen and Dongshan). In this paper we report the full geological context for more ancient rocks from a third Anshan locality – the polyphase migmatite Shengousi Complex. SHRIMP U–Pb zircon dating indicates a protracted tectono-magmatic history for the Shengousi Complex: The oldest recognised component is banded trondhjemitic gneiss (3773 ± 6 Ma), which is veined by strongly deformed granitic pegmatite. These occur with a second generation of trondhjemitic rocks (3454 ± 8 and 3448 ± 9 Ma). The next generation of plutonic rocks is a composite suite of iron-enriched mafic dykes (3332 ± 6 and 3331 ± 8 Ma) with broadly coeval felsic veins (3311 ± 4 Ma). Finally there was intrusion of monzogranite (3129 ± 6 Ma). Strong deformation has generally brought these 3773–3129 Ma plutonic phases into close concordance to form banded rocks. However, locally cross-cutting relationships are preserved in small lower strain domains, that give a relative chronology agreeing with the absolute zircon U–Pb chronology. All of this complex history is recorded in a single \u3c50 m wide outcrop. Some of the \u3c3600 Ma rocks of the Shengousi Complex contain 3780–3730 and 3660–3600 Ma inherited zircon xenocrysts. The stronger prominence of 3660–3600 Ma components distinguishes the Shengousi Complex from the Baijiafen and Dongshan complexes, where components of this age are rarer. One possibility is that at 3660–3600 Ma, the Shengousi Complex was at a deeper crustal level than the Baijiafen and Dongshan complexes, and underwent migmatisation at that time. The ∼3450 Ma igneous phases discovered in the Shengousi Complex are new ages for igneous rocks in the Anshan area. The Precambrian geology of the Anshan area is thus marked by polyphase Eoarchaean orthogneisses, the newly recognised ∼3450 Ma phases, widespread 3360–3300 magmatic activity, found in association with Mesoarchaean plutonic rocks and the Neoarchaean Anshan Group and Palaeoproterozoic Liaohe Group metasedimentary rocks. This shows that the geology of the Anshan area has a strong similarity with the Narryer Gneiss Complex of Western Australia, which contains the polyphase Eoarchaean Meeberrie gneisses, the 3490–3440 Ma Eurada gneiss association, ∼3300 Ma granites and migmatisation, and is intercalated with younger Precambrian metasedimentary rocks of different ages containing Hadean detrital zircons. No other ancient gneiss complexes in the world show such a close match in their history with the Anshan area rocks. This means that the Anshan area is prospective for locating new occurrences of Hadean crustal components intercalated with younger sedimentary rocks

Detection of diseased pine trees in unmanned aerial vehicle images by using deep convolutional neural networks

This study presents a method that uses high-resolution remote sensing images collected by an unmanned aerial vehicle (UAV) and combines MobileNet and Faster R-CNN for detecting diseased pine trees. MobileNet is used to remove backgrounds to reduce the interference of background information. Faster R-CNN is adopted to distinguish between diseased and healthy pine trees. The number of training samples is expanded due to the insufficient number of available UAV images. Experimental results show that the proposed method is better than traditional machine learning approaches, such as support vector machine and AdaBoost, and methods of DCNN, such as Alexnet, Inception and Faster R-CNN. Through sample expansion and background removal, the proposed method achieves effective detection of diseased pine trees in UAV images by using deep learning technology