Finite element analysis of coupled vibration for hoisting cable with time-varying length

The coupled axial-torsional responses of the hoisting cable with time-varying length are investigated in order to predict the longitudinal vibration more accurately. The equations of motion are formulated by Hamilton’s principle and the finite element method (FEM), in which a variable-length cable element is introduced. In order to validate this theoretical model, an ADAMS simulation model is established in the framework of the multi-body system dynamic. The result shows that the numerical solution is in reasonably good agreement with the ADAMS simulation. The frequencies of the cables with the coupling considered and neglected are analyzed by varying the excitation frequency, which indicates that the coupling effect reduces the natural frequency of the cable and the maximum amplitude shifts from the resonance region to the deceleration stage as the coupling coefficient increases

Coupled vibration of hoisting cable in cable-guided hoisting system with different swivels

In most cases, the hoisting cable in the cable-guided hoisting system is connected to the hoisting bucket with the swivel. The coupled longitudinal-torsional responses of the hoisting cable with time-varying length are investigated. The hoisting cable and two guiding cables are discretized by employing the assumed modes method, while the equations of motion are derived using Lagrange equations of the first kind, where a coefficient λ varying from 0 to 1 is introduced to represent the free spinning, proportional and self-locking swivels. The longitudinal and torsional displacements with different swivels are obtained. The results indicate the torsional displacement in the free spinning swivel is much larger than that in the proportional and there is one resonance in the former, while the longitudinal resonance in the free spinning swivel occurs earlier than that in the other two, which implies the system frequencies decrease. In addition, the presented model could also be used to describe the coupled vibration in the rigid rail-guided hoisting system but needs more modes

A novel protein-coding ORF72.2 gene was identified from Marek's disease virus strain CVI988

Marek's disease is a highly contagious disease of poultry characterized by rapid-on set of T-cell lymphomas, which is caused by Marek's disease virus (MDV), but its pathogenic mechanism is still not very clear. Recently, some new progress were achieved in molecular character of MDV. Along with the genomic sequencing of MDV serotype 1, some novel open reading frames (ORFs) were predicted, and ORF72.2 was one of them which have no homologues in other MDV serotypes or in other alphaherpesvirus. In the study, ORF72.2 was firstly identified as a protein-coding gene by the method of reverse transcription polymerase chain reaction (RT-PCR), western blotting and indirect immunofluorescence assay. This study paved the way to conduct further studies to determine whether ORF72.2 plays a role in MDV replication and pathogenicity

Comparative analysis of oncogenic genes revealed unique evolutionary features of field Marek's disease virus prevalent in recent years in China

<p>Abstract</p> <p>Background</p> <p>Marek's disease (MD) is an economically important viral disease of chickens caused by Marek's disease virus (MDV), an oncogenic herpesvirus. This disease was well controlled since the widespread use of commercial vaccines, but field MDVs have shown continuous increasing in virulence and acquired the ability to overcome the immune response induced by vaccines. Nowadays, MD continues to be a serious threat to poultry industry, isolation and characterization of MDVs are essential for monitoring changes of viruses and evaluating the effectiveness of existing vaccines.</p> <p>Results</p> <p>Between 2008 and 2010, 18 field MDV strains were isolated from vaccinated chicken flocks in Sichuan province, China. Three oncogenic genes including Meq, pp38 and vIL-8 genes of the 18 isolates were amplified and sequenced. Homology analysis showed that the deduced amino acid sequences of these three genes exhibit 95.0-98.8%, 99.3-100% and 97.0-98.5% homology respectively with these of other reference strains published in GenBank. Alignment analysis of the nucleotide and deduced amino acid sequences showed that four amino acid mutations in Meq gene and two amino acid mutations in vIL-8 gene displayed perfect regularity in MDVs circulating in China, which could be considered as features of field MDVs prevalent in recent years in China. In addition, one amino acid mutation in pp38 gene can be considered as a feature of virulent MDVs from USA, and three amino acid mutations in Meq gene were identified and unique in very virulent plus (vv+) MDVs. Phylogenetic analysis based on Meq and vIL-8 protein sequences revealed that field MDVs in China evolved independently. Virulence studies showed that CVI988 could provide efficient protection against the field MDVs epidemic recently in China.</p> <p>Conclusions</p> <p>This study and other published data in the GenBank have demonstrated the features of Meq, pp38 and vIL-8 genes of MDVs circulating in recent years in Sichuan, China. Mutations, deletions or insertions were observed in these three genes, and some mutations could be considered as the unique marks of the MDVs circulating presently in China. The paper supplies some valuable information concerning the evolution of MDV which is useful for the vaccine development and control of MD in China.</p

Expression and Functional Analysis of the BCL2-Associated Agonist of Cell Death (BAD) Gene in the Sheep Ovary During the Reproductive Cycle

Most ewes in China are seasonally polyestrous with normal ovulatory cycles, which is controlled by photoperiod (length of the daily light phase). These ewes are estrous in the short-day season and anestrus in the long-day season and cannot mate during anestrus. Thus seasonal breeding limits both diversification and intensification of production. If sheep can estrus all round year, it can be mated twice per year, which can greatly improve the economic benefits. To change seasonal estrus at the genetic level and cultivating new sheep breeds, it is important to understand the molecular mechanisms of seasonal breeding trait in sheep. The BCL2-associated agonist of cell death (BAD) gene being a regulator of cellular apoptosis was identified by our previous RNA-Seq, which is associated with follicular development in mammalian ovaries. However, the mechanism how BAD can regulate estrus in sheep was poorly understood. In this study, we characterized ovine BAD, including full-length mRNA cloning and protein sequence prediction, as well as BAD expression profile in Small-tailed Han (STH) sheep. The highest expression levels of BAD were observed in sheep hypothalamus, lung, and pituitary, while the lowest expression was in liver. Functional analysis of BAD was performed in primary granulosa cells of sheep. The concentration of P4 was significantly increased after RNAi interference of BAD, while P4 level was shown to be opposite after BAD overexpression in vitro. It has been found that BAD can reduce progesterone levels by promoting ovarian GC apoptosis, which might be involved in regulating the estrus cycle in sheep

Cardiac Function Assessment in Fetuses With Ductus Arteriosus Constriction: A Two-Dimensional Echocardiography and FetalHQ Study

BackgroundFetal ductal constriction (DC) is associated with excessive polyphenol-rich food (PRF) consumption during pregnancy. However, the effect of this hemodynamic change on fetal cardiac function still needs to be elucidated. Therefore, this study aimed to evaluate the cardiac function of fetuses with PRF-related DC and to describe serial observations of cardiac function changes.MethodsWe compared the traditional echocardiographic indices, including morphological, hemodynamic, and functional parameters, between study fetuses and controls. For global and segmental deformation analysis of the left and right ventricles, fetalHQ with the speckle-tracking technique was used to calculate sphericity index (SI), global longitudinal strain (GLS), fractional shortening (FS), fractional area change (FAC), etc. In addition, follow-up data were compared with the generalized linear model.ResultsA total of 60 DC fetuses and 60 gestational-matched controls were enrolled in our study, with 20 DC fetuses undertaking a follow-up echocardiogram after 2–3 weeks. Compared with controls, there was a distinct decrease in right ventricular GLS (RVGLS) (−13.39 ± 3.77 vs. −21.59 ± 2.51, p &lt; 0.001), RVFAC (22.20 ± 9.56 vs. 36.01 ± 4.84, p &lt; 0.001), left ventricular GLS (LVGLS) (−19.52 ± 3.24 vs. −23.81 ± 2.01 p &lt; 0.001), and LVFAC (39.64 ± 7.32 vs. 44.89 ± 4.91, p = 0.004). For 24-segment FS analysis, DC fetuses showed lower FS in left ventricular (LV) segments 18–24, with no difference in LV segments 1–17. Right ventricular (RV) FS in segments 4–23 was also reduced in the DC group. The 24-segment SI analysis indicated significantly lower SI in DC than those in controls for LV segments 1–14 and RV segments 19–24. We found that the pulsatility index (PI) of ductus arteriosus (DA) was an independent variable for RVGLS (β = −0.29, p = 0.04). In 20 DC fetuses with follow-up echocardiograms, no obvious difference in myocardial deformation was found between the initial examination and follow-up data.ConclusionLeft and right ventricular performances were both impaired in DC fetuses, along with a series of morphological and hemodynamic changes. Although the state of DA constriction improved on second examinations, cardiac function was not completely restored

Vertical Transportation Diversity of Petroleum Pollutants under Groundwater Fluctuations and the Instructions for Remediation Strategy

Based on the information from an actual petroleum-contaminated site, a one-dimensional soil column was used to examine the vertical transportation diversities of different petroleum components under the influence of water table fluctuations, and the results revealed the following: (1) There were two obvious pollution accumulation zones under the condition of water table fluctuations: (i) The pollution infiltration zone dominated by the residual phase was formed at the leakage position, and (ii) the floating zone dominated by the free phase was formed near the water table. Combined with the viscosity of the organic components, the concentrations of the residual phase were octanoic acid > hexadecane > cyclohexane > toluene. Compared to coarse sand, clay can adsorb more components. (2) Different fluctuation frequencies had a great influence on the migration process of components. The free phase can transfer into the residual phase during the low groundwater table fluctuation. In the case of high-frequency groundwater fluctuations, there were more free phase components that can be carried by the water flow. However, due to the continuous flushing by the water, pollutants were finally spread to the whole underground system. (3) A cost-effectiveness remediation strategy is based on the difference in pollutant transportation. Therefore, the conclusions in this paper are fully applied in the actual contaminated sites. Specifically, the air-sparing (AS) and soil vapor extraction (SVE) devices were installed in the vadose zone to remove volatile substances (such as toluene). The permeable reactive barriers (PRBs) were set in the groundwater fluctuation zone to repair the residual pollutants (alkanes, cyclane, and asphaltenes hydrocarbons) that are continuously converted from the residual phase to the dissolved phase and free phase due to water level fluctuations. Hence, the results of this study provided a specific, targeted, and comprehensive strategy for petroleum pollution treatment