抗NHE10モノクローナル抗体は破骨細胞の形成過程における細胞融合を抑制する

内容の要旨 , 審査の要旨広島大学(Hiroshima University)博士(口腔健康科学)Philosophy in Oral Health Sciencedoctora

Assembly Sequence Planning by Probabilistic Tree Transformation

Various types of computer systems including CAD/CAM systems have been introduced in machine industry. Some of the systems can handle assembly sequence planning, however it requires long time for planning. This paper proposes a method of generating assembly sequences efficiently. This method extracts some parts and/or subassemblies whose possibilities of being removed from a product are strong, and tests whether they can be removed without any geometric interference. By performing these operations repeatedly, the method generates a disassembly sequence of the product, and obtains an assembly sequence by reversing it. The extraction of some parts and/or subassemblies is performed, based on probabilistic tree transformation. The authors present a calculation example by using a software tool integrated with a CAD system

Titanium Immobilized with an Antimicrobial Peptide Derived from Histatin Accelerates the Differentiation of Osteoblastic Cell Line, MC3T3-E1

The objective of this study was to evaluate the effect of titanium immobilized with a cationic antimicrobial peptide (JH8194) derived from histatin on the biofilm formation of Porphyromonas gingivalis and differentiation of osteoblastic cells (MC3T3-E1). The titanium specimens (Ti) were immobilized with JH8194, according to the method previously described. The colonization of P. gingivalis on JH8194-Ti was significantly lower than that on control- and blocking-Ti. JH8194-Ti enhanced the mRNA expressions of Runx2 and OPN, and ALPase activity in the MC3T3-E1, as compared with those of control- and blocking-Ti. These results, taken together, suggested the possibility that JH8194-Ti may be a potential aid to shorten the period of acquiring osseointegration

Transition and trend of study on domestic and overseas anorexia and dysphagia

The purpose of this study is to survey domestic and overseas studies on anorexia and dysphagia and explore their transition and characteristics in each generation. The authors performed a word frequency analysis and a characteristic words analysis by generation using the text mining method for the titles of total 1,638 original papers published in published in the past 33 years in “Dysphagia”, which is an academic journal for swallowing disorders. For studies on swallowing, its examination methods and swallowing function evaluations were mainly conducted in the1980-1990s, and case reports about swallowing training methods and systematic reviews increased after the 2000s. Moreover, themes for cerebrovascular disorder patients and cancer patients attract researchers’ attention accompanied with global aging. One of the characteristics of the swallowing support system in Japan is that multiple professionals are involved with it

Impact of the Microgravity Environment in a Three–dimensional Clinostat on Osteoblast- and Osteoclast-like Cells

Mechanical unloading conditions result in decreases in bone mineral density and quantity, which may be partly attributed to an imbalance in bone formation and resorption. To investigate the effect of mechanical unloading on osteoblast and osteoclast differentiation, and the expression of RANKL and OPG genes in osteoblasts, we used a three-dimensional (3D) clinostat system simulating microgravity to culture MC3T3-E1 and RAW264.7 cells. Long-term exposure (7 days) of MC3T3-E1 cells to microgravity in the 3D clinostat inhibited the expression of Runx2, Osterix, type I collagen αI chain, RANKL and OPG genes. Similarly, 3D clinostat exposure inhibited the enhancement of β3-integrin gene expression, which normally induced by sRANKL stimulation in RAW264.7 cells. These results, taken together, demonstrate that long-term 3D clinostat exposure inhibits the differentiation of MC3T3-E1 cells together with suppression of RANKL and OPG gene expression, as well as the RANKL-dependent cellular fusion of RAW264.7 cells, suggesting that long-term mechanical unloading suppresses bone formation and resorption