1 research outputs found
Effect of Trolox addition to cryopreservation media on human sperm motility
Background: Sperm parameters and motion kinetics are affected by
cryopreservation. Objective: The main purpose of the current study was
to determine the effect of different concentrations of Trolox as an
antioxidant to freezing-thawing procedure on human sperm kinematic
parameter. Materials and Methods: Semen was collected from 20 normal
donors and divided into five aliquots prior to cryopreservation. The
first aliquot was analyzed by computer-assisted sperm analysis (CASA).
Other aliquots were mixed with cryo-protective agent containing 0, 20,
40, and 80 μmol Trolox and treated samples were cryopreserved in
liquid nitrogen. After two weeks samples were thawed and sperm motion
kinematics was measured by CASA. Percent motility (Mot), curvilinear
velocity (VCL), straight-line velocity (VSL), average path velocity
(VAP), linearity (LIN), and amplitude of lateral head displacement
(ALH) were compared before and after freeze. Results: Addition of
40μmol Trolox resulted in significantly higher (p<0.05) post
thaw VCL, VSL and VAP compared to other groups. Therefore the
percentage of post thaw motile spermatozoa were significantly higher
(p<0.01). Conclusion: The supplementation of Trolox significantly
improved the post-thawed human semen quality, especially progressive
motility and average path velocity