Preparation of biologically active polyphenols by utilising the primrose (Primula veris), the horsetail (Equisetum arvense) and the yarrow (Achillea millefolium) processing waste.

Predmet ove disertacije je iskorišćenje otpadnih sirovina koje su dobijene u procesu prerade jagorčevine (Primula veris), rastavića (Equisetum arvense) i hajdučke trave (Achillea millefolium) za dobijanje biološki aktivnih ekstrakata bogatih polifenolima...The subject of this dissertation is the utilization of waste materials from the processing of primrose (Primula veris), horsetail (Equisetum arvense) and yarrow (Achillea millefolium) for obtaining biologically active and polyphenol-rich extracts..

The role of central vasopressin V2 receptors in cardiovascular homeostasis

Poznato je da vazopresin modulira nervnu regulaciju kardiovaskularnog sistema centralno i periferno, ali tačan mehanizam još uvek je nepoznat. Da bi ispitali da li centralni V2 receptori vazopresina modifikuju nervnu regulaciju kardiovaskularnog sistema ispitivali smo efekte centralno ubrizganog selektivnog antagoniste V2 receptora na hemodinamske parametre: krvni pritisak, srčanu frekvenciju i njihov varijabilitet, pod bazalnim fiziološkim uslovima i u stresu. Pored toga, pod bazalnim fiziološkim uslovima ispitali smo efekat hiperekspresije V2 receptora u paraventrikularnom jedru hipotalamusa na hemodinamske parametre neanestetisanog pacova. Krvni pritisak i srčana frekvencija registrovani su direktnom metodom preko katetera postavljenog u femoralnu arteriju ili radiotelemetrijskog transmitera u aortu. Nervna regulacija krvnog pritiska i srčane frekevencije evaluirana je spektralnom metodom. Pod bazalnim fiziološkim uslovima antagonist V2 vazopresinskih receptora nije modifikovao krvni pritisak i srčanu frekvenciju, ali je izazvao porast oscilacija veoma niskih frekvencija krvnog pritiska. Hiperekspresija V2 receptora u paraventrikularnom jedru hipotalamusa pacova izazvala je porast niskofrekventnog varijabiliteta simpatičkog porekla kako u krvnom pritisku, tako i u srčanoj frekvenciji. Stres izazvan mlazom vazduha pod pritiskom usmeren u potiljak pacova prouzrokovao je istovremeni porast krvnog pritiska i srčane frekvencije, kao i njihovog niskofrekventnog varijabiliteta simpatičkog porekla i visokofrekventnog varijabiliteta respiratornog porekla. Pacovi pretretirani antagonistom V2 receptora i potom izlagani stresu ispoljili su manji skok krvnog pritiska i varijabiliteta simpatičkog i respiratornog porekla, kao i kraći oporavak. Rezultati pokazuju da vazopresin putem stimulacije centralnih V2 receptora utiče na kardiorespiratorni odgovor na stres.It is well established that vasopressin modulates neurogenic control of the circulation both centrally and peripherally. However the exact mechanism is still unexplored. To investigate whether central V2 receptors modulate the neurogenic control of the circulation, we studied the effects of centrally injected selective V2 antagonist on hemodynamic parameters: blood pressure (BP), heart rate (HR) and their variability, under baseline physiological conditions and during stress. We also investigated the effects of over-expression of V2 receptors in the paraventricular nucelus of the hypothalamus on cardiovascular hemodynamic under baseline conditions. BP and HR were assessed by direct recording via indwelling catheter in femoral artery or by radiotelemetric probe inserted in abdominal aorta. Neurogenic control of the circulation was assessed by spectral methodology. Under baseline physiological conditons V2 antagonist didn't modify the level of BP and HR and increased the very low frequency variability of BP. Over-expression of V2 receptors in the paraventricular nucleus increased sympathetically mediated, low-frequency oscillation of BP and HR. Exposure of rats to air-jet stress increased BP, HR and their sympahtetically mediated low-frequency variability and respiration-induced high-frequency variability. Rats pre-treated with the V2 antagonist and exposed to stress exhibited lower BP increase and lower increase od BP variability, as well as shorter recovery time. It follows that vasopressin by the stimulation of central V2 receptors influence the cardiorespiratory response to stress

Characterization of nanostructured spinel NiFe2O4 obtained by soft mechanochemical synthesis

Powdery nickel ferrite, NiFe2O4 has been obtained by soft mechanochemical synthesis in a planetary ball mill. Ni(OH)2 and Fe(OH)3 are used as initial compounds. This mixture was mechanically activated for 25h, uniaxial pressed and sintered at 1100°C for 2h. The phase composition of the sintered sample was analyzed by X-ray diffraction (XRD), energy dispersive spectrometer (EDS) and Raman spectroscopy. Morphologies were examined by scanning electron microscopy (SEM). The electrical DC/resistivity/conductivity at different temperatures was measured using a Source Meter Keithley 2410. An Impedance/Gain-Phase Analyzer (HP-4194) was used to measure the impedance spectra (100Hz - 10MHz) at different temperatures. [Projekat Ministarstva nauke Republike Srbije, br. III 45003 i br. III 45015

Development of a sampling plan for Myzus persicae (Hemiptera : Aphidoidea) and its predator Macrolophus costalis (Hemiptera : Miridae) on tobacco

A tobacco field in Greece was sampled during the 2001 and 2002 growing seasons to assess the seasonal trends in densities and spatial distributions of the aphid Myzus persicae (Sulzer) and its predatory mirid Macrolophus costalis (Fieber). On repeated occasions between June Oust after the transplantation) and September Oust before harvest), 20 tobacco leaves (10 from the upper and 10 from the lower plant part) were taken from randomly chosen plants. These leaves were sampled for aphids and mirids. In both years, the highest aphid densities were recorded during July and August, while aphid numbers were low in September. In contrast, the majority of M costalis individuals were found during September when aphid numbers were low. Significantly more M persieae individuals were found in the upper part of the plants, whereas significantly more M costalis individuals were found in the lower part of the plants. As indicated by Taylor's Power Law estimates, both species were aggregated in their spatial distributions among sampling units (leaves). Wilson and Room's model, based on the Taylor's estimates, was used to calculate the mean number of aphids and mirids, from the proportion of sampling units (leaves) that had gt 0 individuals of each species. This model provided a satisfactory fit of the data for both the aphid and the mirid. In addition, Wilson and Room's model was successfully used to predict the mean number of aphids and mirids in a series of samples that were carried out in the same area between June and September 2003 for model validation. Finally, equations are given for the calculation of precision in estimating the mean number of aphids or mirids per sampling unit, and the required sample size for a given level of precision

Measurement of the σ x BR(H→ZZ*) at 350 GeV and 3 TeV center-of-mass energies CLIC

In this paper we present results of the determination of the statistical precision of the branching fraction measurement, for Higgs decaying to ∗ pairs at 3 TeV and 350 GeV CLIC. Measurements are simulated with the CLIC_ILD detector model, taking into consideration all relevant physics and beam-induced background processes. It is shown that the product of the branching fraction ( → ∗ ) and the Higgs production cross-section can be measured with a relative statistical uncertainty of 3% (20%) at 3 TeV (350 GeV) center-of-mass energy, using semileptonic final states and assuming an integrated luminosity of 5 (1) ab−1 .BPU11 : 11th International Conference of the Balkan Physical Union : Proceedings book; Aug 11 - Sep 1, 2022S05-HEP High Energy Physics (Particles and Fields

Third mission of the university – basic differences in approaches

Tradicionalne i osnovne misije sveučilišta, nastava i istraživanje sve se više proširuju. Treća misija sveučilišta je višedimenzionalan koncept koji poprima široke razmjere na svjetskom nivou. Kao cilj rada postavlja se proučavanje osnovnih sličnosti i razlika u pristupima trećoj misiji sveučilišta kada je riječ o američkim u odnosu na europska sveučilišta. Kao polazna osnova za usporedbu uzima se činjenica da treća misija sveučilišta svoje porijeklo ima na američkom kontinentu dok se nastanak sveučilišta vezuje za Europu. Cilj rada je konkretiziran na sljedeće zadatke: ispitati utjecaj globalnih obrazovnih politika na pristupe trećoj misiji sveučilišta; ispitati razlike u načinu funkcioniranja europskih u odnosu na američka sveučilišta; objasniti obrazovne implikacije provedbe treće misije na europskim sveučilištima. Kao zaključak rada ističe se da je neophodno razumijevanje globalnih društveno-ekonomskih utjecaja na proces visokog obrazovanja kako bi se sveobuhvatnije pristupilo obrazovnim reformama i načinima njihovog sprovođenja. Također, s obzirom na sudjelovanje sve brojnijih interesnih grupa u okviru realizacije treće misije sveučilišta, neminovno je razvijanje stavova o vrijednostima i načelima treće misije kod svih onih koji su uključeni u proces visokog obrazovanja.The traditional and basic missions of the university, teaching and research are expanding. Terminology on the third mission of the university is gaining wide scope at the global level. The aim of this paper is to study basic similarities and differences in approach to the third mission of the university by comparing American to European universities. The starting point for comparing these approaches is the fact that the third mission of the university has its origins in the American continent, while the creation of the university is linked to Europe. The aim of this paper is to specify the following tasks: to examine the impact of global education policies on approaches to the third mission of the university; to examine the differences in the way European universities operate in relation to American universities; to explain the educational implications of the implementation of the university’s third mission at European universities. In conclusion, it is necessary to understand the global socioeconomic impacts on the process of higher education in order to approach educational reforms and ways of implementing them more comprehensively. Also, given the growing number of the participating interest groups in the implementation of the third mission of the university, it is inevitable to develop attitudes about the values and principles of the third mission among all those who are involved in the process of higher education

Impact of bee venom and melittin on apoptosis and biotransformation in colorectal carcinoma cell lines

© 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group. This study provides data about anticancer properties of bee venom and its dominant compound, melittin on colorectal carcinoma cells (HCT-116 and SW-480) in regard to their proapoptotic activity and expression of genes involved in biotransformation process. Based on results, they are strong cytotoxins, where the melittin showed also selectivity against cancer cells compared to normal, HaCat. They induce proapoptotic activity by affecting apoptosis signaling molecules (Fas receptors, caspase 9, and members of Bcl-2 family of proteins) and mainly suppress expression of genes involved in their biotransformation, suggesting their ability to develop the resistance of colorectal cancer cells

Primena metode informacionih spektara u ispitivanju enzimatskih različitih alfa amilaza

A series of eight alpha amylases (EC 3.2.1.1) of known primary structure were investigated using the Informational Spectrum Method (ISM) for the purpose of predicting new enzymes with increased efficiency.Niz od 8 α-amilaza (1,4-α-D-glukan-glukano -hidrolaze, EC 3.2.1.1) poznate primarne strukture ispitivano je metodom informacionih spektara (ISM) , s ciljem da se predlože novi enzimi veće efikasnosti. Pomoću ISM-a transformisana je primarna struktura enzima u niz brojeva, a zatim je bilo moguće dobiti i uporediti originalne spektre. Korišćenjem multi-spektralne funkcije utvrđen je samo jedan zajednički, dobro definisan pik. Frekvenca ovog pika predstavlja karakterističnu frekvencu svih ispitivanih amilaza. Postoji zavisnost između vrednosti amplitude na karakterističnoj frekvenciji i aktivnosti biomolekula. Daljom matematičkom obradom, metoda je dala predlog za minimalne promene u strukturi prirodnog proizvoda kojima se omogućava povećana efikasnost enzima. Ove minimalne promene moguće je izvesti najjednostavnijom metodom genetskog inženjerstva - metodom tačkaste mutacije. Mutacije su predložene za α-amilaze iz sledećih izvora: Aspergillus oryzae, Bacillus arnyloliquefaciens i ječam

Kinematika kompleksne višeosne i viševretene mašine alatke namenjene za proces obrade glodanjem

U okviru ovog rada je prikazana analiza jedne višeosne i viševretene mašine alatke namenjene za proces obrade glodanjem. Prema svojoj konfiguraciji, razmatrana mašina alatka se može okarakterisati kao glodalica horizontalno-vertikalnog tipa što je svrstava u grupu multifunkcionalnih mašina alatki. Mašina alatka se prema više kriterijuma može okarakterisati kao mašina alatka rekonfigurabilnog tipa i u zavisnosti od potreba se može konfigurisati za 3-osnu, 4-osnu ili 5-osnu obradu glodanjem. Konfiguracija mašine alatke poseduje dva nezavisna dvoosna rekonfigurabilna paralelna mehanizma što samu mašinu alatku svrstava u grupu kompleksnih mašina alatki