6,301 research outputs found
The process of infection with bacteriophage phiX174, XXX. Replication of double-stranded phiX DNA
Intermediates involved in the replication of double-stranded phiX174 RF DNA have been identified and partially characterized. Analysis of pulse-labeled RF DNA suggests that the synthesis of progeny RF molecules involves, in part, the addition of nucleotides to linear complementary strands on a circular parental strand as template, so as to produce intermediate DNA strands of greater than viral length. Electron microscopy reveals DNA rings with "tails" and "double rings," which could be the intermediate structures. A model is postulated for the replication process
Review: emerging concepts in the pathogenesis of tendinopathy
Tendinopathy is a common clinical problem and has a significant disease burden attached, not only in terms of health care costs, but also for patients directly in terms of time off work and impact upon quality of life. Controversy surrounds the pathogenesis of tendinopathy, however the recent systematic analysis of the evidence has demonstrated that many of the claims of an absence of inflammation in tendinopathy were more based around belief than robust scientific data. This review is a summary of the emerging research in this topical area, with a particular focus on the role of neuronal regulation and inflammation in tendinopathy
Rapid virological surveillance of community influenza infection in general practice
No abstract available
Low loss Ge-on-Si waveguides operating in the 8–14 µm atmospheric transmission window
Germanium-on-silicon waveguides were modeled, fabricated and characterized at wavelengths ranging from 7.5 to 11 µm. Measured waveguide losses are below 5 dB/cm for both TE and TM polarization and reach values of ∼ 1 dB/cm for ≥ 10 µm wavelengths for the TE polarization. This work demonstrates experimentally for the first time that Ge-on-Si is a viable waveguide platform for sensing in the molecular fingerprint spectral region. Detailed modeling and analysis is presented to identify the various loss contributions, showing that with practical techniques losses below 1 dB/cm could be achieved across the full measurement range
The adsorption and desorption of ethanol ices from a model grain surface
Reflection absorption infrared spectroscopy (RAIRS) and temperature programed desorption (TPD) have been used to probe the adsorption and desorption of ethanol on highly ordered pyrolytic graphite (HOPG) at 98 K. RAIR spectra for ethanol show that it forms physisorbed multilayers on the surface at 98 K. Annealing multilayer ethanol ices (exposures > 50 L) beyond 120 K gives rise to a change in morphology before crystallization within the ice occurs. TPD shows that ethanol adsorbs and desorbs molecularly on the HOPG surface and shows four different species in desorption. At low coverage, desorption of monolayer ethanol is observed and is described by first-order kinetics. With increasing coverage, a second TPD peak is observed at a lower temperature, which is assigned to an ethanol bilayer. When the coverage is further increased, a second multilayer, less strongly bound to the underlying ethanol ice film, is observed. This peak dominates the TPD spectra with increasing coverage and is characterized by fractional-order kinetics and a desorption energy of 56.3 +/- 1.7 kJ mol(-1). At exposures exceeding 50 L, formation of crystalline ethanol is also observed as a high temperature shoulder on the TPD spectrum at 160 K. (c) 2008 American Institute of Physics
Sulphur-bearing molecules in AGB stars I: The occurrence of hydrogen sulfide
Through a survey of (sub-)millimetre emission lines of various
sulphur-bearing molecules, we aim to determine which molecules are the primary
carriers of sulphur in different types of AGB stars. In this paper, the first
in a series, we investigate the occurrence of HS in AGB circumstellar
envelopes and determine its abundance, where possible. We have surveyed 20 AGB
stars with a range of mass-loss rates and of different chemical types using the
APEX telescope to search for rotational transition lines of five key
sulphur-bearing molecules: CS, SiS, SO, SO and HS. Here we present our
results for HS, including detections, non-detections and detailed radiative
transfer modelling of the detected lines. We compare results based on different
descriptions of the molecular excitation of HS and different abundance
distributions, including those derived from chemical modelling results. We
detected HS towards five AGB stars, all of which have high mass-loss rates
of yr and are oxygen-rich. HS
was not detected towards the carbon or S-type stars that fall in a similar
mass-loss range. For the stars in our sample with detections, we find peak
o-HS abundances relative to H between and . Overall, we conclude that HS can play a significant role in
oxygen-rich AGB stars with higher mass-loss rates, but is unlikely to play a
key role in stars of other chemical types or the lower mass-loss rate
oxygen-rich stars. For two sources, V1300 Aql and GX Mon, HS is most likely
the dominant sulphur-bearing molecule in the circumstellar envelope.Comment: 14 pages, 7 figures, accepted in A&
- …