The process of infection with bacteriophage phiX174, XXX. Replication of double-stranded phiX DNA

Intermediates involved in the replication of double-stranded phiX174 RF DNA have been identified and partially characterized. Analysis of pulse-labeled RF DNA suggests that the synthesis of progeny RF molecules involves, in part, the addition of nucleotides to linear complementary strands on a circular parental strand as template, so as to produce intermediate DNA strands of greater than viral length. Electron microscopy reveals DNA rings with "tails" and "double rings," which could be the intermediate structures. A model is postulated for the replication process

Review: emerging concepts in the pathogenesis of tendinopathy

Tendinopathy is a common clinical problem and has a significant disease burden attached, not only in terms of health care costs, but also for patients directly in terms of time off work and impact upon quality of life. Controversy surrounds the pathogenesis of tendinopathy, however the recent systematic analysis of the evidence has demonstrated that many of the claims of an absence of inflammation in tendinopathy were more based around belief than robust scientific data. This review is a summary of the emerging research in this topical area, with a particular focus on the role of neuronal regulation and inflammation in tendinopathy

Rapid virological surveillance of community influenza infection in general practice

No abstract available

Low loss Ge-on-Si waveguides operating in the 8–14 µm atmospheric transmission window

Germanium-on-silicon waveguides were modeled, fabricated and characterized at wavelengths ranging from 7.5 to 11 µm. Measured waveguide losses are below 5 dB/cm for both TE and TM polarization and reach values of ∼ 1 dB/cm for ≥ 10 µm wavelengths for the TE polarization. This work demonstrates experimentally for the first time that Ge-on-Si is a viable waveguide platform for sensing in the molecular fingerprint spectral region. Detailed modeling and analysis is presented to identify the various loss contributions, showing that with practical techniques losses below 1 dB/cm could be achieved across the full measurement range

The adsorption and desorption of ethanol ices from a model grain surface

Reflection absorption infrared spectroscopy (RAIRS) and temperature programed desorption (TPD) have been used to probe the adsorption and desorption of ethanol on highly ordered pyrolytic graphite (HOPG) at 98 K. RAIR spectra for ethanol show that it forms physisorbed multilayers on the surface at 98 K. Annealing multilayer ethanol ices (exposures > 50 L) beyond 120 K gives rise to a change in morphology before crystallization within the ice occurs. TPD shows that ethanol adsorbs and desorbs molecularly on the HOPG surface and shows four different species in desorption. At low coverage, desorption of monolayer ethanol is observed and is described by first-order kinetics. With increasing coverage, a second TPD peak is observed at a lower temperature, which is assigned to an ethanol bilayer. When the coverage is further increased, a second multilayer, less strongly bound to the underlying ethanol ice film, is observed. This peak dominates the TPD spectra with increasing coverage and is characterized by fractional-order kinetics and a desorption energy of 56.3 +/- 1.7 kJ mol(-1). At exposures exceeding 50 L, formation of crystalline ethanol is also observed as a high temperature shoulder on the TPD spectrum at 160 K. (c) 2008 American Institute of Physics

Sulphur-bearing molecules in AGB stars I: The occurrence of hydrogen sulfide

Through a survey of (sub-)millimetre emission lines of various sulphur-bearing molecules, we aim to determine which molecules are the primary carriers of sulphur in different types of AGB stars. In this paper, the first in a series, we investigate the occurrence of H$_2$S in AGB circumstellar envelopes and determine its abundance, where possible. We have surveyed 20 AGB stars with a range of mass-loss rates and of different chemical types using the APEX telescope to search for rotational transition lines of five key sulphur-bearing molecules: CS, SiS, SO, SO$_2$ and H$_2$S. Here we present our results for H$_2$S, including detections, non-detections and detailed radiative transfer modelling of the detected lines. We compare results based on different descriptions of the molecular excitation of H$_2$S and different abundance distributions, including those derived from chemical modelling results. We detected H$_2$S towards five AGB stars, all of which have high mass-loss rates of $\dot{M}\geq 5\times 10^{-6}M_\odot$ yr$^{-1}$ and are oxygen-rich. H$_2$S was not detected towards the carbon or S-type stars that fall in a similar mass-loss range. For the stars in our sample with detections, we find peak o-H$_2$S abundances relative to H$_2$ between $4\times 10^{-7}$ and $2.5\times 10^{-5}$. Overall, we conclude that H$_2$S can play a significant role in oxygen-rich AGB stars with higher mass-loss rates, but is unlikely to play a key role in stars of other chemical types or the lower mass-loss rate oxygen-rich stars. For two sources, V1300 Aql and GX Mon, H$_2$S is most likely the dominant sulphur-bearing molecule in the circumstellar envelope.Comment: 14 pages, 7 figures, accepted in A&