Expression des "activated leukocyte cell adhesion molecule" im Mammakarzinom: Prädiktivität für das Ansprechen auf eine taxanfreie Chemotherapie

Zusammenfassung: Ziele: "Activated leukocyte cell adhesion molecule" (ALCAM) ist ein Oberflächenimmunglobulin und wird in vielen Mammakarzinomen exprimiert. Es wird vermutet, dass ALCAM in der Tumorgenese und -progression eine Rolle spielt. Die Bedeutung des Adhäsionsmoleküls ALCAM für das Ansprechen auf eine taxanfreie adjuvante Chemotherapie wurde untersucht. Material und Methoden: Gewebeproben von 162Patientinnen mit Mammakarzinomen wurden im Hinblick auf die Expression des ALCAM-Proteins untersucht. Immunhistologische Untersuchungen (IHC) und Western-Blot-Analysen (WB) wurden mit einem monoklonalen Antikörper gegen ALCAM durchgeführt. Die Proteinmengen im WB wurden densitometrisch quantifiziert und mit klinischen sowie histologischen Parametern korreliert. Die Ergebnisse wurden auf mRNA-Ebene mithilfe der Microarray-Analysen (Affymetrix) validiert. Ergebnisse: In der normalen Brustdrüse wird ALCAM in den luminalen und den basalen Epithelzellen exprimiert. Die WB-Analysen von Mammakarzinomen zeigen eine positive Korrelation der ALCAM-Expression mit dem Östrogenrezeptorstatus (p=0,04). Bei Patientinnen, die eine taxanfreie Chemotherapie erhalten haben, ist eine hohe ALCAM-Expression (WB, Affymetrix) prädikitiv für das Ansprechen einer Chemotherapie. Der mediane mRNA-Level von ALCAM war bei Patientinnen, die während des Beobachtungszeitraums noch lebten, 4,5-fach höher als bei Patientinnen, die verstarben. Schlussfolgerung: Ein hoher ALCAM-Gehalt korreliert positiv mit dem Östrogenrezeptorstatus. Außerdem ist ALCAM ein prädiktiver Faktor für das Ansprechen einer taxanfreien Chemotherapi

Numerical verification of universality for the Anderson transition

We analyze the scaling behavior of the higher Lyapunov exponents at the Anderson transition. We estimate the critical exponent and verify its universality and that of the critical conductance distribution for box, Gaussian and Lorentzian distributions of the random potential

Expression of Rb2/p130 in breast and endometrial cancer: correlations with hormone receptor status

Rb2/p130 is a member of the retinoblastoma family of proteins, consisting of Rb, Rb2 and p107, which are important negative regulators of cell cycle progression and differentiation. While Rb2 downregulation was observed in several malignant tumours including endometrial cancer, the role of p130 in breast carcinomas is still unknown. We investigated Rb2 protein expression in tumour tissue from 68 mammary and 41 endometrial carcinomas, 4 mammary cell lines, and normal tissue samples. Therefore, we performed Western blot experiments for Rb2, Rb, and the oestrogen and progesterone receptors (ER, PR-A, PR-B). Weak or absent Rb2 expression was more often found in endometrial (59%) than in mammary carcinomas (24%). We found significant positive correlations of Rb2 expression with Rb, ER, and PR-B in breast cancer samples, and of Rb2 with Rb, PR-A, PR-B, and younger age in endometrial carcinomas. No significant associations with histological grading, stage, nodal involvement, or Ki67 staining were detected. Rb2 mRNA expression was studied by semi-quantitative RT-PCR in 56 endometrial or mammary tissue samples and correlated significantly with Western blot results. Our results indicate that loss of Rb2 expression, mostly by transcriptional down-regulation, may be associated with the development and dedifferentiation of most endometrial and a subset of mammary carcinomas. © 2001 Cancer Research Campaign http://bjcancer.co

Scaling of the conductance distribution near the Anderson transition

The single parameter scaling hypothesis is the foundation of our understanding of the Anderson transition. However, the conductance of a disordered system is a fluctuating quantity which does not obey a one parameter scaling law. It is essential to investigate the scaling of the full conductance distribution to establish the scaling hypothesis. We present a clear cut numerical demonstration that the conductance distribution indeed obeys one parameter scaling near the Anderson transition

Energy-level statistics at the metal-insulator transition in anisotropic systems

We study the three-dimensional Anderson model of localization with anisotropic hopping, i.e. weakly coupled chains and weakly coupled planes. In our extensive numerical study we identify and characterize the metal-insulator transition using energy-level statistics. The values of the critical disorder $W_c$ are consistent with results of previous studies, including the transfer-matrix method and multifractal analysis of the wave functions. $W_c$ decreases from its isotropic value with a power law as a function of anisotropy. Using high accuracy data for large system sizes we estimate the critical exponent $\nu=1.45\pm0.2$. This is in agreement with its value in the isotropic case and in other models of the orthogonal universality class. The critical level statistics which is independent of the system size at the transition changes from its isotropic form towards the Poisson statistics with increasing anisotropy.Comment: 22 pages, including 8 figures, revtex few typos corrected, added journal referenc

The three-dimensional Anderson model of localization with binary random potential

We study the three-dimensional two-band Anderson model of localization and compare our results to experimental results for amorphous metallic alloys (AMA). Using the transfer-matrix method, we identify and characterize the metal-insulator transitions as functions of Fermi level position, band broadening due to disorder and concentration of alloy composition. The appropriate phase diagrams of regions of extended and localized electronic states are studied and qualitative agreement with AMA such as Ti-Ni and Ti-Cu metallic glasses is found. We estimate the critical exponents nu_W, nu_E and nu_x when either disorder W, energy E or concentration x is varied, respectively. All our results are compatible with the universal value nu ~ 1.6 obtained in the single-band Anderson model.Comment: 9 RevTeX4 pages with 11 .eps figures included, submitted to PR