In Vivo

In vivo real-time visualization of the process of angiogenesis in secondary tumors in the same living animals presents a major challenge in metastasis research. We developed a technique for intravital imaging of colorectal liver metastasis development in live mice using two-photon laser scanning microscopy (TPLSM). We also developed time-series TPLSM in which intravital TPLSM procedures were performed several times over periods of days to months. Red fluorescent protein-expressing colorectal cancer cells were inoculated into the spleens of green fluorescent protein-expressing mice. First- and second-round intravital TPLSM allowed visualization of viable cancer cells (red) in hepatic sinusoids or the space of Disse. Third-round intravital TPLSM demonstrated liver metastatic colonies consisting of viable cancer cells and surrounding stroma with tumor vessels (green). In vivo time-course imaging of tumor angiogenesis in the same living mice using time-series TPLSM could be an ideal tool for antiangiogenic drug evaluation, reducing the effects of interindividual variation

Kondo effect in coupled quantum dots under magnetic fields

The Kondo effect in coupled quantum dots is investigated theoretically under magnetic fields. We show that the magnetoconductance (MC) illustrates peak structures of the Kondo resonant spectra. When the dot-dot tunneling coupling $V_C$ is smaller than the dot-lead coupling $\Delta$ (level broadening), the Kondo resonant levels appear at the Fermi level ($E_F$). The Zeeman splitting of the levels weakens the Kondo effect, which results in a negative MC. When $V_{C}$ is larger than $\Delta$, the Kondo resonances form bonding and anti-bonding levels, located below and above $E_F$, respectively. We observe a positive MC since the Zeeman splitting increases the overlap between the levels at $E_F$. In the presence of the antiferromagnetic spin coupling between the dots, the sign of MC can change as a function of the gate voltage.Comment: 6 pages, 3 figure

Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment

Li J., Hua Y., Liu Y., et al. Human induced pluripotent stem cell-derived closed-loop cardiac tissue for drug assessment. iScience 27, 108992 (2024); https://doi.org/10.1016/j.isci.2024.108992.Human iPSC-derived cardiomyocytes (hiPSC-CMs) exhibit functional immaturity, potentially impacting their suitability for assessing drug proarrhythmic potential. We previously devised a traveling wave (TW) system to promote maturation in 3D cardiac tissue. To align with current drug assessment paradigms (CiPA and JiCSA), necessitating a 2D monolayer cardiac tissue, we integrated the TW system with a multi-electrode array. This gave rise to a hiPSC-derived closed-loop cardiac tissue (iCT), enabling spontaneous TW initiation and swift pacing of cardiomyocytes from various cell lines. The TW-paced cardiomyocytes demonstrated heightened sarcomeric and functional maturation, exhibiting enhanced response to isoproterenol. Moreover, these cells showcased diminished sensitivity to verapamil and maintained low arrhythmia rates with ranolazine—two drugs associated with a low risk of torsades de pointes (TdP). Notably, the TW group displayed increased arrhythmia rates with high and intermediate risk TdP drugs (quinidine and pimozide), underscoring the potential utility of this system in drug assessment applications

The Far-Infrared Surveyor (FIS) for AKARI

The Far-Infrared Surveyor (FIS) is one of two focal plane instruments on the AKARI satellite. FIS has four photometric bands at 65, 90, 140, and 160 um, and uses two kinds of array detectors. The FIS arrays and optics are designed to sweep the sky with high spatial resolution and redundancy. The actual scan width is more than eight arcmin, and the pixel pitch is matches the diffraction limit of the telescope. Derived point spread functions (PSFs) from observations of asteroids are similar to the optical model. Significant excesses, however, are clearly seen around tails of the PSFs, whose contributions are about 30% of the total power. All FIS functions are operating well in orbit, and its performance meets the laboratory characterizations, except for the two longer wavelength bands, which are not performing as well as characterized. Furthermore, the FIS has a spectroscopic capability using a Fourier transform spectrometer (FTS). Because the FTS takes advantage of the optics and detectors of the photometer, it can simultaneously make a spectral map. This paper summarizes the in-flight technical and operational performance of the FIS.Comment: 23 pages, 10 figures, and 2 tables. Accepted for publication in the AKARI special issue of the Publications of the Astronomical Society of Japa

Kondo resonant spectra in coupled quantum dots

The Kondo effect in coupled quantum dots is investigated from the viewpoint of transmission spectroscopy using the slave-boson formalism of the Anderson model. The antiferromagnetic spin-spin coupling $J$ between the dots is taken into account. Conductance $G$ through the dots connected in a series is characterized by the competition between the dot-dot tunneling coupling $V_{C}$ and the level broadening $\Delta$ in the dots (dot-lead coupling). When $V_{C}/\Delta < 1$, the Kondo resonance is formed between each dot and lead, which is replaced by a spin-singlet state in the dots at low gate voltages. The gate voltage dependence of $G$ has a sharp peak of $2 e^2/h$ in height in the crossover region between the Kondo and spin-singlet states. The sharp peak of $G$ survives when the energy levels are different between the dots. When $V_{C} / \Delta > 1$, the "molecular levels" between the Kondo resonant states appear; the Kondo resonant peaks are located below and above the Fermi level in the leads at low gate voltages. The gate voltage dependence of $G$ has a broad peak, which is robust against $J$. The broad peak splits into two peaks when the energy levels are different, reflecting the formation of the asymmetric molecular levels between the Kondo resonant states.Comment: 21 pages, 8 figures, to appear in Phys. Rev.