Draft Genome of Bugula neritina, a Colonial Animal Packing Powerful Symbionts and Potential Medicines

Many animal phyla have no representatives within the catalog of whole metazoan genome sequences. This dataset fills in one gap in the genome knowledge of animal phyla with a draft genome of Bugula neritina (phylum Bryozoa). Interest in this species spans ecology and biomedical sciences because B. neritina is the natural source of bioactive compounds called bryostatins. Here we present a draft assembly of the B. neritina genome obtained from PacBio and Illumina HiSeq data, as well as genes and proteins predicted de novo and verified using transcriptome data, along with the functional annotation. These sequences will permit a better understanding of host-symbiont interactions at the genomic level, and also contribute additional phylogenomic markers to evaluate Lophophorate or Lophotrochozoa phylogenetic relationships. The effort also fits well with plans to ultimately sequence all orders of the Metazoa

Genome-wide association study reveals genetic variants associated with HIV-1C infection in a Botswana study population

Although there have been many studies of gene variant association with different stages of HIV/AIDS progression in United States and European cohorts, few gene-association studies have assessed genic determinants in sub-Saharan African populations, which have the highest density of HIV infections worldwide. We carried out genome-wide association studies on 766 study participants at risk for HIV-1 subtype C (HIV-1C) infection in Botswana. Three gene associations (AP3B1, PTPRA, and NEO1) were shown to have significant association with HIV-1C acquisition. Each gene association was replicated within Botswana or in the United States–African American or United States–European American AIDS cohorts or in both. Each associated gene has a prior reported influence on HIV/AIDS pathogenesis. Thirteen previously discovered AIDS restriction genes were further replicated in the Botswana cohorts, extending our confidence in these prior AIDS restriction gene reports. This work presents an early step toward the identification of genetic variants associated with and affecting HIV acquisition or AIDS progression in the understudied HIV-1C afflicted Botswana population

Prevalence of Risk Factors of Thromboembolic Complications in Women after Major Joint Arthroplasty in the Republic of Sakha (Yakutia)

The aim of this study was to assess the risk factors for thromboembolic complications after total arthroplasty of large joints in women in Yakutia conditions to optimize the management tactics of this category of patients. The average age of women was 59.98±11.56 years in the age range from 50 to 70 years. In order to validate the study, women were divided into 2 groups. The main group consisted of 284 women undergoing total knee arthroplasty (Group 1). The comparison group included 147 women undergoing total hip arthoplasty (Group 2). The study demonstrated that hypertension was more common in patients of Group 1 than in patients of Group 2. However, the incidence of coronary heart disease and heart rhythm disorder was detected most frequently in patients with total hip arthroplasty. Obesity, thrombosis of the veins of the lower extremities, and liver disease were detected with almost the same frequency in women with total knee arthroplasty and those with total hip arthroplasty. The frequency of occurrence of complications depending on the risk factors for thromboembolic complications and the type of surgical treatment of the joint was equal in the two groups of studied patients

Genome-wide sequence analyses of ethnic populations across Russia

The Russian Federation is the largest and one of the most ethnically diverse countries in the world, however no centralized reference database of genetic variation exists to date. Such data are crucial for medical genetics and essential for studying population history. The Genome Russia Project aims at filling this gap by performing whole genome sequencing and analysis of peoples of the Russian Federation. Here we report the characterization of genome-wide variation of 264 healthy adults, including 60 newly sequenced samples. People of Russia carry known and novel genetic variants of adaptive, clinical and functional consequence that in many cases show allele frequency divergence from neighboring populations. Population genetics analyses revealed six phylogeographic partitions among indigenous ethnicities corresponding to their geographic locales. This study presents a characterization of population-specific genomic variation in Russia with results important for medical genetics and for understanding the dynamic population history of the world's largest country

Instrument facilities of integration and optimization of graphic information representation in data bases of computer-aided design

Purpose of the work: development of the language, algorithms and programs for the optimum representation of the graphic information in the data bases of the computer-aided design. Developed are the language and methods for the optimum representation of the graphic information in the data bases of the computer-aided design. Programs are developed for the support of the data graphic interpretation language "GID" and for formation of the optimum representation of the graphic information at the language level and at the level of a metafile. Methods are proposed for utilization of the software tools. The software is introduced in Production Association "Tochmash" and Scientific and Production Association "Kompas"Available from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio

Siltation and radiocesium pollution of small lakes in different catchment types far from the Fukushima Daiichi nuclear power plant accident site

The Fukushima Daiichi nuclear power plant accident caused radioactive pollution in northeastern Honshu Island, Japan. This study examined the influence of snowmelt and rainfall on soil erosion processes and siltation of small lakes in Miyagi Prefecture (150 km northwest of the power plant). Two sets of slopes and lakes, respectively in pasture and forest catchments, were examined. Snowpack thickness, soil infiltration, surface runoff volume, soil and sediment physicochemical properties, Cs concentration of precipitation, meltwater, and rainwater, and lake siltation rates were determined. The total radioactive Cs content in precipitation was 0.7–7.4 BqˑL−1 and was below the Japanese standard (10 BqˑL−1). Total radioactive Cs was at the allowable level in water flowing down the pasture catchment slope (0.1–9.2 BqˑL−1) during snowmelt and rainfall, as well as in pasture (0.9–8.8 BqˑL−1) and forest (0.7–5.2 BqˑL−1) catchment lake water. There was no soil erosion (surface runoff) in the forest catchment. Soil losses in the pasture catchment were 23 due to rainfall and 9 kg ha−1 yr−1 following spring snowmelt. After snowmelt, a 0.5 and 0.2 mm thick layer of silt was deposited in pasture and forest catchment lakes, respectively, and 1.4 and 0.6 mm were deposited during the rainfall period. Average siltation rates were 1.9 and 0.8 mmˑyr−1 for pasture and forest catchment lakes, respectively. The upper layer of lake bottom sediments is represented mainly by silt fractions (2–50 μm), with high organic matter (4.0–5.7%) and radiocesium (1100–1600 kgˑha−1) contents. Keywords: Fukushima Daiichi, Radiocesium, Siltation, Suspended sediment, Waterbodies, Water turbidit

Digital mapping of solonchak complexes using Sentinel-2A data

Land salinization is an up-to-date issue being broadly studied all over the world. In Russia, salinization processes are predominantly observed in the southern regions, where the main areas of arable land are situated. This research is devoted to mapping of saline lands with the help of satellite data. The study was performed on a 100-hectare plot in the Trans-Ural steppe zone (Republic of Bashkortostan, Russia). A correlation was determined between the level of soil salinity and the main spectral indices associated with Sentinel-2A satellite data. Regression models used 5 salinity indices, vegetation index NDVI, and values of soil conductivity. Linear, quadratic, and logarithmic functions were used. By calculation, the salinity index 5 (G×R)/B demonstrated the best correlation values with the salinity level of (R=0.88, R2=0.77) while using the quadratic function. The vegetation index NDVI revealed no correlation, owing to the poor development or dried-up condition of vegetation. On the basis of the developed regression models, salinity maps are drawn, in which the areas of solonchak complexes are defined

Variations in soil erodibility (K-factor) for the Chernozems depending on the method of texture determination

Soil erodibility (K-factor) is an important parameter in erosion modeling, is one of five factors of the Revised Universal Soil Loss Equation (RUSLE), and generally represents the soil's response to rainfall and run-off erosivity. The erodibility could be determined based on direct measurements of soil properties and mathematical calculations. In this study, the K-factor was calculated based on a formula from RUSLE, proposed by Renard et al. (1997). All input parameters: soil organic carbon (SOC), soil structure, and permeability classes were measured by one method, but particle size distribution – in two ways by sedimentation and laser diffraction methods to assess the impact the K-factor variability and the values of soil erosion rates. The 107 soil samples of Chernozems from Kursk Oblast (Russia) were studied. The texture for the most of samples was classified as silty loam in both analyses. However, the laser diffraction underestimates the clay content by an average of 13.2 % compared to the pipette method. The average K-factor estimated based on laser diffraction data was 0.050, and 0.034 t ha h ha−1 MJ−1 mm−1 – sedimentation method. Thus, depending on the method of soil texture analysis, the RUSLE calculated soil loss could underestimated/overstated by 32 % (or 4 t ha-1 yr-1 on average in the study site). Therefore, we propose a regression equation-based conversion method of laser diffraction data to sedimentation method data for Chernozems. • The Laska-TM laser analyzer measured on ∼ 13 % less clay fraction (more on ∼ 8 % silt and ∼ 5 % fine sand) compared with sedimentation method data. • For erosional researchers/modelers it is suggested to state the method of soil texture analysis (based on sedimentation law or laser diffraction) was used for RUSLE K-factor calculations. • To convert K-factor values (for Chernozems) calculated and based on data of the sedimentation method to laser sedimentation – it suggested utilize the coefficient 1.47 (0.68 – vice versa)

Contemporary Long-Term Trends in Water Discharge, Suspended Sediment Load, and Erosion Intensity in River Basins of the North Caucasus Region, SW Russia

For the first time, contemporary trends in water discharge, suspended sediment load, and the intensity of overall erosion in the river basins of the North Caucasus region, as one of Russia’s most agriculturally developed geographic areas, were identified. The study was carried out using monitoring data of the Federal Service for Hydrometeorology and Environmental Monitoring of the country for 21 rivers by comparing two periods: 1963–1980 and 2008–2017. According to the study’s results, trends of an increase in the mean annual water discharge (by 2–97%) and the essential reduction in its intra-annual variability have been found in most of the studied rivers. On the contrary, the trends of reduction in annual suspended sediment load and the intensity of erosion in the river basins were identified in most of the study region. Their most essential and statistically significant decreases (by 47–94%) were recorded within the Stavropol Upland, which several decades ago was considered one of the most erosion-dangerous territories of the entire country, as well as in some river basins of the central part of the Greater Caucasus’s northern slope (by 17–94%). The changes in climate (reducing the depth of soil freezing and meltwater runoff on the soil) and land use/cover (reduction of acreage and load (pressure) of agricultural machinery on the soil, reducing livestock on pastures, and the transfer of water from the neighboring, more full-flowing rivers) are considered the leading causes of the aforementioned trends. The findings will contribute to solving some economic and environmental problems of both the region and adjacent territories and water areas