Almost as helpful as good theory: Some conceptual possibilities for the online classroom

Interest and activity in the use of C&IT in higher education is growing, and while there is effort to understand the complexity of the transition to virtual space, aspects of development, particularly clarity about the nature of the learning community, may only be lightly theorized. Based on an ongoing action research study involving postgraduate students studying in the UK and USA, this paper will identify some theoretical roots and derive from these six conceptual areas that seem to the authors to have relevance and significance for behaviour online. An exploration of these forms the basis for a two‐dimensional model which can account for what happens when groups come together to learn in cyberspace. In depicting this model, there is acknowledgement of the existence of third and fourth dimensions at work. However, the explanatory power of taking these extra dimensions into account is beyond the scope of the analysis thus far

Time to experiment: A response

It is with some pleasure that we were given the opportunity to offer this paper for commentary and we are grateful for the efforts made by readers to help us to refine our thinking. Given the constraints of space, we will respond to the main comments in turn. We plan to submit a more considered and elegant paper to a future edition when we have worked more on our model

Contingent Valuation and Social Choice

JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact [email protected]. How can you measure the net benefits to society from actions that impact environmental resources? An economist's answer is to employ Hicksian consumer surplus, determining the equivalent variation in income that leaves each consumer indifferent to the action. When consumers are rational and consumer surplus can be measured reliably from market demand functions, this is a satisfactory basis for welfare calculation, subject to the customary caveats about distributional equity and consistency if compensation is not actually paid. When externalities, public goods, or informational asymmetries interfere with the determination of consumer surplus from market demand functions, one can try to set up a hypothetical market to elicit an individual's equivalent variation, or willingness-to-pay (WTP). This is called the contingent valuation method (CVM). The approach elicits stated preferences from a sample of consumers using either openended questions that ask directly for WTP, or referendum (closed-ended) questions that present a bid or a sequence of bids to the consumer, and ask for a yes or no vote on whether each bid exceeds the subject's WTP. A single referendum experiment presents only one bid; a double referendum experiment presents a second bid that is conditioned on the subject's response to the first bid, lower if the first response is no and higher if it is yes. Agricultural & Applied Economics Association and Oxford University Press An extensive literature has investigated the use of CVM to value environmental goods, and in recent years has promoted it for evaluation of goods such as endangered species and wilderness areas whose value comes primarily from existence rather than active use.' The typical CVM experiment in environmental economics asks about a single commodity, often with a fairly abbreviated or stylized description that assumes the consumer can draw upon prior knowledge. Typically, there is no training of the consumer to reduce inconsistent (e.g. In assessing CVM, there are three commonsense questions that can be asked: (a) Is the method psychometrically robust, in that results cannot be altered substantively by changes in survey format, questionnaire design, and instructions that should be inconsequential when behavior is driven by maximization of rational preferences? (b) Is the method statistically reliable, in that the distribution of WTP can be estimated with acceptable precision using practical sample sizes? Reliability is a particular issue if CV surveys produce extreme responses with some probability, perhaps due to strategic misrepresentation. (c) Is the method economically sensible, in that the individual preferences measured by CVM are consistent with the logical requirements of rationality (e.g., transitivity), and at least broadly consistent with sensible features of economic preferences (e.g., plausible budget shares and income elasticities)? CVM might fail to meet these criteria because respondents receive incomplete information on the consequences of the available choices, or are given inadequate incentives to be truthful and avoid strategic misrepresentation, or because the experimental design is not sufficiently rich to detect and compensate for systematic and random response errors. Beyond such technical problems, there could be a fundamental failure of CVM if consumers do not have stable, classical preferences for the class of commodities, so that the foundations of Hicksian welfare analysis break down. Intuitively, the further removed a class of commodities from market goods where the consumer has the experience of repeated choices and the discipline of market forces, the greater the possibility of both technical and fundamental failures. The broad sweep of evidence from market research, cognitive psychology, and experimental economics suggests that the existence value of natural resources, involving very complex commodities that are far outside consumers' market experience, will be vulnerable to these failures (McFadden 1986). The following sections discuss, in turn, a series of statistical issues in analyzing WTP data, parametric methods for estimating mean WTP, an experiment that was designed to detect and quantify technical failures of CVM, and the results from the experiment. Using referendum questions complicates matters only slightly, since votes at a sufficiently broad and closely spaced range of bid levels can be used to estimate directly the distribution of WTP, and this in turn can be used to estimate the population mean. This claim is proved in McFadden (1994), which gives practical nonparametric estimators, and describes the restrictions necessary on referendum experimental design for these estimators to have good largesample properties. In overview, the result is that with truthful referendum data there are estimators whose mean square error is inversely proportional to sample size, provided the experimental design "undersmooths" by taking a relatively large number of bid levels, with relatively small samples at each bid.2 For example, when WTP is restricted a priori to a finite interval, one could distribute the bids evenly over this interval, with one respondent at each level. The common practice in CV referendum studies of taking a relatively small number of bid levels leads to estimators whose mean square errors decline more slowly with sample size. Statistical Issues in CV Data 2 When the support of the WTP distribution is not finite, additional restrictions on tail behavior are needed to assure the existence of mean WTP and the stated rate of convergence of nonparametric estimators

Identification of a Putative Crf Splice Variant and Generation of Recombinant Antibodies for the Specific Detection of Aspergillus fumigatus

BACKGROUND: Aspergillus fumigatus is a common airborne fungal pathogen for humans. It frequently causes an invasive aspergillosis (IA) in immunocompromised patients with poor prognosis. Potent antifungal drugs are very expensive and cause serious adverse effects. Their correct application requires an early and specific diagnosis of IA, which is still not properly achievable. This work aims to a specific detection of A. fumigatus by immunofluorescence and the generation of recombinant antibodies for the detection of A. fumigatus by ELISA. RESULTS: The A. fumigatus antigen Crf2 was isolated from a human patient with proven IA. It is a novel variant of a group of surface proteins (Crf1, Asp f9, Asp f16) which belong to the glycosylhydrolase family. Single chain fragment variables (scFvs) were obtained by phage display from a human naive antibody gene library and an immune antibody gene library generated from a macaque immunized with recombinant Crf2. Two different selection strategies were performed and shown to influence the selection of scFvs recognizing the Crf2 antigen in its native conformation. Using these antibodies, Crf2 was localized in growing hyphae of A. fumigatus but not in spores. In addition, the antibodies allowed differentiation between A. fumigatus and related Aspergillus species or Candida albicans by immunofluorescence microscopy. The scFv antibody clones were further characterized for their affinity, the nature of their epitope, their serum stability and their detection limit of Crf2 in human serum. CONCLUSION: Crf2 and the corresponding recombinant antibodies offer a novel approach for the early diagnostics of IA caused by A. fumigatus

Honeyfiles: Deceptive Files for Intrusion Detection

Proceedings of the 2004 IEEE Workshop on Information Assurance, U.S. Military Academy, West Point, NY, June 2004

Identification of novel genes conferring altered azole susceptibility in Aspergillus fumigatus

Azoles are currently the mainstay of antifungal treatment both in agricultural and clinical settings. Although the target site of azole action is well studied the basis of azole resistance and the ultimate mode of action of the drug in fungi is poorly understood. In order to gain a deeper insight into these aspects of azole action restriction mediated plasmid integration (REMI) was used to create azole sensitive and resistant strains of the clinically important fungus Aspergillus fumigatus. Four azole sensitive insertions and 4 azole resistant insertions were characterised. Three phenotypes could be re-created in wild type AF210 by reintegration of rescued plasmid and a further 4 could be confirmed by complementation of the mutant phenotype with a copy of the wild type gene predicted to be disrupted by the original insertional event. Six insertions were in genes not previously associated with azole sensitivity or resistance. Two insertions occur in transporter genes that may affect drug efflux whereas others may affect transcriptional regulation of sterol biosynthesis genes and NADH metabolism in the mitochondrion. Two insertions are in genes of unknown function