成人女性の生活意識と将来展望 -喪失感と獲得感の予備的検討-

40歳〜60歳代の中年期女性50名に質問紙調査を実施し、 現在の生活状況、 生活意識の実態について把握し、 将来展望との関連を検討した。 さらに、 これからの人生で 「失うもの」 と 「得るもの」 に対する意識を自由記述により把握し、 その内容の分析から、 喪失感と獲得感について検討した。 その結果、 現在の暮らし向き、 健康状態、 主観的幸福感は、 ポジティブな時間的展望と有意な関連を示した。 「喪失」 と 「獲得」 の内容は、 心身の機能、 家族を含めた人間関係、 時間、 仕事・経済力、 自己の内面的な変化に大別された。 これからの先の人生について、 「喪失」 と 「獲得」 の両者を含んだ将来展望を抱いていることが確認された。We conducted a questionnaire survey on 50 middle-aged women aged ≧40 years to analyze the relationship between life consciousness and future outlook. The contents of the consciousness of loss and acquisition in the remainder of the subjects’ lives reported in free response format were also investigated. Current life circumstances, health status, and subjective well-being were significantly related to a positive time perspective. Regarding future outlook, subjects had both consciousness of loss and acquisition

ショウガッコウ ガイコクゴ カツドウ ニオケル ヒョウカ ニ カンスル ジッセン ケンキュウ : キョウイク ジッセン フィールド ケンキュウ ニ モトズイテ

鳴門教育大学大学院では，学校現場における今日的課題について協力学校とともに実践的研究を行う授業「実践フィールド研究」が設定されている。本実践報告では，平成21年度大学院生による「実践フィールド研究」に基づいた実践を報告するものである。平成23年度の小学校外国語活動の本格実施に先駆けて，本学附属小学校では長年英語学習として関連授業に取り組んできた。しかし，評価に関する課題についてはまだ十分検討されておらず本実践において，その可能性について実践研究を行うこととした。また，前年度の継続研究として，全国全ての5，6年生に配付された『英語ノート』の活用についても併せて取り組んでおり，本報告ではこの二点について報告する。特に評価に関しては，最も小学校現場で多く使われている「自己評価」のあり方，実施の際の留意点について検討するとともに「学びのための自己評価」の可能性についても提案する。最後に，次年度へ向けての改訂版振り返りカードの提案も行う

3.3 Agricultural Science. Effects of Ce and Methanol on Growth and Expression of Methanol Dehydrogenase of Bradyrhizobium japonicum USDA110 and Its Enzymatic Properties

Abstract. Rare earth elements (REEs) have been widely used in high-technology products such as computers, mobile telephones, plasma displays, magneto-optical disks, high-powered lasers fluorescent lamps and hybrid cars (1). Increasing demand of REEs may cause the environmental pollution by these elements. Despite importance of physics and chemistry of REEs, the significance of REEs in biology has been overlooked. In our laboratory, during the study on the relationships between REEs and microorganism we isolated a soil bacterium, identified as Bradyrhizobium sp. CE-3 whose methanol dehydrogenase (MDH) activity in crude cell-free extract was increased several times when grown in 1/10 nutrient medium containing Ce. This is of interest that REEs exhibited the physiological effects on enzyme expression of microorganisms. Bradyrhizobium japonicum USDA110 has been widely used as standard to investigate the physiological, biochemical and genetic characterizations of the genus Bradyrhizobium. Kaneko et al. published the complete genomic nucleotide sequence of B. japonicum USDA110 and pointed out that this bacterium has a gene encoding a MDH large subunits-like protein at blr locus 6213 (2). In this report, I describe the effects of Ce and methanol on growth and expression of MDH activity of B. japonicum USDA 110, and that purification and some properties of the enzyme. Growth behaviors and MDH activity: The 1/10 diluted nutrient medium was used in this study. Methanol and Ce were added at 0.5% and 30 μM, respectively. Bacterium was cultured with rotary shaker (120 rpm) at 300 Growth of B. japonicum USDA110 exhibited remarkable increase in the presence of both 30 μM Ce and 0.5% methanol. This results suggest that Ce is important role in methanol metabolism of the genus Bradyrhizobium. In addition, activity of MDH was remarkably increased by Ce and methanol. MDHs of Methylobacterium spp. were found to increase several times by Ce. It seems that Ce is involved in induction of MDHs of methylotrophic bacteria. C. Cells were harvested by centrifugation, washed with 20mM Tris-HCl buffer (pH 8.0), and suspended in the same buffer. Cells were disrupted by sonication. After centrifugation, the supernatant was used as cellfree extract. Activity of MDH was determined according to method of Day and Anthony (3). Protein concentration was determined by using BCA Protein Assay Kit. Purification of MDH: Ce-induced MDH was purified by three purification steps. The enzyme was purified 16 fold with yield of 7% and migrated as a single band (67 kDa) on SDS-PAGE (4). Properties of purified MDH: By gel chromatography MW of native MDH was estimated to be approximately 86 kDa. This result suggests that the MDH was monomer. Recently the monomeric MDH which was a product of xoxF (synonym mxaF’) gene of Methylobacterium extorquens AM1 was reported (5). The enzyme was more active to primary alcohols such as ethanol (93%), 1-Propanol (93%), and 1-butanol (93%) rather than secondary alcohols such as 2-propanol (36%) and 2-buthanol (36%), relative to methanol (100%). The pH and temperature optimum was 9.0 and 35C, respectively. Km for methanol and Vmax were determined to be 0.32 mM and 11.0 U/mg protein by Lineweaver-Burk plots. The N-terminal amino acid sequence was determined to be 1-NDELHKMAQNPKDWVMP-17. The amino acid sequence was highly identity to that of deduced amino acid sequence of MDH large subunit-like protein encoded by mxaF’ (3). Keywords: Ce, methanol dehydrogenase, Bradyrhizobium japonicum, methano

Lumbar Intervertebral Disc Degeneration Does Not Affect Muscle Synergy for Rowing Activities

Rowers with disc degeneration may have motor control dysfunction during rowing. This study is aimed at clarifying the trunk and lower extremity muscle synergy during rowing and at comparing the muscle synergy between elite rowers with and without lumbar intervertebral disc degeneration. Twelve elite collegiate rowers (with disc degeneration, n=6; without disc degeneration, n=6) were included in this study. Midline sagittal images obtained by lumbar T2-weighted magnetic resonance imaging were used to evaluate disc degeneration. Participants with one or more degenerated discs were classified into the disc degeneration group. A 2000 m race trial using a rowing ergometer was conducted. Surface electrodes were attached to the right rectus abdominis, external oblique, internal oblique, latissimus dorsi, multifidus, erector spinae, rectus femoris, and biceps femoris. The activity of the muscles was measured during one stroke immediately after 20% and 80% of the rowing trial. Nonnegative matrix factorization was used to extract the muscle synergies from the electromyographic data. To compare the muscle synergies, a scalar product (SP) evaluating synergy coincidence was calculated, and the muscle synergies were considered identical at SP>75%. Both groups had only one module in the 20% and 80% time points of the trial. At the 20% time point of the 2000 m rowing trial, the SP of the module was 99.8%. At the 80% time point, the SP of the module was 99.9%. The SP results indicate that, at 20% and 80% time points, both groups had the same module. The module showed a high contribution in all muscles. The activation coefficients indicated that the module was always highly activated throughout the rowing stroke in both groups. The trunk and lower extremity muscles are mobilized through the rowing stroke and maintain coordination during rowing. There was no difference in the muscle synergy between the rowers with and without lumbar intervertebral disc degeneration

LSD1-LIKE1-Mediated H3K4me2 Demethylation Is Required for Homologous Recombination Repair

International audienceHomologous recombination is a key process for maintaining genome integrity and diversity. In eukaryotes, the nucleosome structure of chromatin inhibits the progression of homologous recombination. The DNA repair and recombination protein RAD54 alters the chromatin structure via nucleosome sliding to enable homology searches. For homologous recombination to progress, appropriate recruitment and dissociation of RAD54 is required at the site of homologous recombination; however, little is known about the mechanism regulating RAD54 dynamics in chromatin. Here, we reveal that the histone demethylase LYSINE-SPECIFIC DEMETHYLASE1-LIKE 1 (LDL1) regulates the dissociation of RAD54 at damaged sites during homologous recombination repair in the somatic cells of Arabidopsis (Arabidopsis thaliana). Depletion of LDL1 leads to an overaccumulation of RAD54 at damaged sites with DNA double-strand breaks. Moreover, RAD54 accumulates at damaged sites by recognizing histone H3 Lys 4 di-methylation (H3K4me2); the frequency of the interaction between RAD54 and H3K4me2 increased in the ldl1 mutant with DNA double-strand breaks. We propose that LDL1 removes RAD54 at damaged sites by demethylating H3K4me2 during homologous recombination repair and thereby maintains genome stability in Arabidopsis