Structure and hydration of membranes embedded with voltage-sensing domains.

Despite the growing number of atomic-resolution membrane protein structures, direct structural information about proteins in their native membrane environment is scarce. This problem is particularly relevant in the case of the highly charged S1-S4 voltage-sensing domains responsible for nerve impulses, where interactions with the lipid bilayer are critical for the function of voltage-activated ion channels. Here we use neutron diffraction, solid-state nuclear magnetic resonance (NMR) spectroscopy and molecular dynamics simulations to investigate the structure and hydration of bilayer membranes containing S1-S4 voltage-sensing domains. Our results show that voltage sensors adopt transmembrane orientations and cause a modest reshaping of the surrounding lipid bilayer, and that water molecules intimately interact with the protein within the membrane. These structural findings indicate that voltage sensors have evolved to interact with the lipid membrane while keeping energetic and structural perturbations to a minimum, and that water penetrates the membrane, to hydrate charged residues and shape the transmembrane electric field

Characterization of PLD grown WO3 thin films for gas sensing

Tungsten trioxide (WO3) thin films were grown by pulsed laser deposition (PLD) with the aim to be applied in gas sensors. The films were studied by atomic force microscopy (AFM), X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy and profilometry. To study the gas sensing behavior of these WO3 films, they were deposited on quartz resonators and the quartz crystal microbalance (QCM) method was applied to analyze their gas sensitivity. Synthesis of tetragonal-WO3 films starting from a target with predominantly monoclinic WO3 phase was observed. The films deposited at 300 degrees C presented a surface topology favorable for the sorption properties, consisting of a film matrix with protruding craters/cavities. QCM prototype sensors with such films were tested for NO2 sensing. The PLD grown WO3 thin films show good sensitivity and fast reaction at room temperature, even in as-deposited state. With the presented technology, the manufacturing of QCM gas sensors is simple, fast and cost-effective, and it is also suitable for energy-effective portable equipment for on-line monitoring of environmental changes. (C) 2017 Elsevier B.V. All rights reserved

Worldsheet correlators in AdS(3)/CFT(2)

The AdS_3/CFT_2 correspondence is checked beyond the supergravity approximation by comparing correlation functions. To this end we calculate 2- and 3-point functions on the sphere of certain chiral primary operators for strings on AdS_3 x S^3 x T^4. These results are then compared with the corresponding amplitudes in the dual 2-dimensional conformal field theory. In the limit of small string coupling, where the sphere diagrams dominate the string perturbation series, beautiful agreement is found.Comment: 23 page

Can Quantum de Sitter Space Have Finite Entropy?

If one tries to view de Sitter as a true (as opposed to a meta-stable) vacuum, there is a tension between the finiteness of its entropy and the infinite-dimensionality of its Hilbert space. We invetsigate the viability of one proposal to reconcile this tension using $q$-deformation. After defining a differential geometry on the quantum de Sitter space, we try to constrain the value of the deformation parameter by imposing the condition that in the undeformed limit, we want the real form of the (inherently complex) quantum group to reduce to the usual SO(4,1) of de Sitter. We find that this forces $q$ to be a real number. Since it is known that quantum groups have finite-dimensional representations only for $q=$ root of unity, this suggests that standard $q$-deformations cannot give rise to finite dimensional Hilbert spaces, ruling out finite entropy for q-deformed de Sitter.Comment: 10 pages, v2: references added, v3: minor corrections, abstract and title made more in-line with the result, v4: published versio

Single molecule imaging reveals the concerted release of myosin from regulated thin filaments

Regulated thin filaments (RTFs) tightly control striated muscle contraction through calcium binding to troponin, which enables tropomyosin to expose myosin-binding sites on actin. Myosin binding holds tropomyosin in an open position, exposing more myosin-binding sites on actin, leading to cooperative activation. At lower calcium levels, troponin and tropomyosin turn off the thin filament; however, this is antagonised by the high local concentration of myosin, questioning how the thin filament relaxes. To provide molecular details of deactivation, we used single-molecule imaging of green fluorescent protein (GFP)-tagged myosin-S1 (S1-GFP) to follow the activation of RTF tightropes. In sub-maximal activation conditions, RTFs are not fully active, enabling direct observation of deactivation in real time. We observed that myosin binding occurs in a stochastic step-wise fashion; however, an unexpectedly large probability of multiple contemporaneous detachments is observed. This suggests that deactivation of the thin filament is a coordinated active process

Spectral Flow in AdS(3)/CFT(2)

We study the spectral flowed sectors of the H3 WZW model in the context of the holographic duality between type IIB string theory in AdS(3)x S^3 x T^4 with NSNS flux and the symmetric product orbifold of T^4. We construct explicitly the physical vertex operators in the flowed sectors that belong to short representations of the superalgebra, thus completing the bulk-to-boundary dictionary for 1/2 BPS states. We perform a partial calculation of the string three-point functions of these operators. A complete calculation would require the three-point couplings of non-extremal flowed operators in the H3 WZW model, which are at present unavailable. In the unflowed sector, perfect agreement has recently been found between the bulk and boundary three-point functions of 1/2 BPS operators. Assuming that this agreement persists in the flowed sectors, we determine certain unknown three-point couplings in the H3 WZW model in terms of three-point couplings of affine descendants in the SU(2) WZW model.Comment: 50 pages, 2 figure