ヒト2, 4-dienoyl-CoA reductaseの遺伝子多型解析

Human 2, 4-dienoyl-CoA reductase (DECR) is an auxiliary enzyme of β-oxidation, participates in the metabolism of unsaturated fatty enoyl-CoA esters having double bonds in both even- and odd-numbered position. DECR gene is approximately 30kb and comprises 10 exons. Four single nucleotide polymorphisms (SNPs) of human DECR have been reported on SNP database, but any SNPs of DECR in the Japanese population have not been determined. To establish a rapid and sensitive means of detecting DECR SNPs, we analyzed those of 200 Japanese individuals using denaturing HPLC (DHPLC). We detected a novel SNP 43441G>A (Ala295Thr) in exon 8. The allele frequency of this polymorphism in the Japanese population appears to be extremely low (0.5%)

ヒトCYP4B1 mRNA発現の臓器差

The cytochrome P450 enzymes (GYP) play important roles in the metabolism of many therapeutic agents and environmental compounds. Differences in the activities of these enzymes are thought to be responsible for individual variability in drug response and/or toxicity. Among the GYP enzymes, the isoform CYP4B1 is of particular interest and involved in the metabolism of several protoxins such as 2-aminoanthracene and 2-aminofluorene, all of which are bladder carcinogens. The present study compared the relative expression pattern of CYP4B1 in cDNA panels of human tissues. We developed an assay for CYP4B1 mRNA by performing real-time RT-PCR. Using this method, CYP4B1 mRNA levels were analyzed in human tissues. CYP4B1 mRNA was selectively expressed in lung, bladder, and prostate. These findings suggest that a high expression of CYP4B1 increases the risk of tumor by activation of carcinogenic compounds