Light-dependent transport processes in isolated membranes of Rhodospeudomonas spaeroides.

The phototrophic bacterium Rhodopseudemonas ashaeroides can obtain the energy for the uptake of amino acids from aerobic metabolism and from the absorption of light (Chapter II). The conversion of light energy into a chemical useful form is accomplished by a photosynthetic apparatus wich is located in the cytoplasmic membrane and in invaginations of this membrane. In order to study light-dependent transport processes through the cytoplasmic membrane in more detail isolated membranes had to be used. Methods were developed for the isolation of membranes from cells. Chapter II describes the isolation of cytoplasmic membrane versicles from cells by an osmotic shock of spheroplast. These membrane can perform energy-dependent accumulation of amino acids. In vesicles isolated from cells grown aerobically in the dark transport of amino acids can be energized by aerobic oxidation of grown anaerobically in the light energy for active transport can be supplied by illumination. ... Zie: Summary