Reliability and validity of pediatric triage tools evaluated in Low resource settings: a systematic review

Background: Despite the high burden of pediatric mortality from preventable conditions in low and middle income countries and the existence of multiple tools to prioritize critically ill children in low-resource settings, no analysis exists of the reliability and validity of these tools in identifying critically ill children in these scenarios. Methods: The authors performed a systematic search of the peer-reviewed literature published, for studies pertaining to for triage and IMCI in low and middle-income countries in English language, from January 01, 2000 to October 22, 2013. An updated literature search was performed on on July 1, 2015. The databases searched included the Cochrane Library, EMBASE, Medline, PubMed and Web of Science. Only studies that presented data on the reliability and validity evaluations of triage tool were included in this review. Two independent reviewers utilized a data abstraction tool to collect data on demographics, triage tool components and the reliability and validity data and summary findings for each triage tool assessed. Results: Of the 4,717 studies searched, seven studies evaluating triage tools and 10 studies evaluating IMCI were included. There were wide varieties in method for assessing reliability and validity, with different settings, outcome metrics and statistical methods. Conclusions: Studies evaluating triage tools for pediatric patients in low and middle income countries are scarce. Furthermore the methodology utilized in the conduct of these studies varies greatly and does not allow for the comparison of tools across study sites

Destination anywhere

Profiles various Maine boating companies that offer harbor, lighthouse, educational, or party cruises at various locations along the Maine coast

Stories of encounters with famous people in Maine, including actor Sarah Jessica

Stories of encounters with famous people in Maine, including actor Sarah Jessica Parker, artist and musician Yoko Ono, actor Tippi Hedren, author Toni Morrison, actor Alec Baldwin, Red Sox player Jacoby Ellsbury, actor Willem Dafoe, actor Kelly Preston, actor Eva Marie Saint, and attorney F. Lee Bailey, who gives an interview

Interview with Ian Crocker, graduate of Cheverus High School in Portland and win

Interview with Ian Crocker, graduate of Cheverus High School in Portland and winner of five Olympic swimming medals. He talks about swimming in Guantanamo Bay, Cuba, and opening a swim school for children in Austin, Texas, where he lives

Short essay on the schooner Bowdoin, which crossed the Arctic circle over two

Short essay on the schooner Bowdoin, which crossed the Arctic circle over two dozen times. In 1988, the Maine Maritime Academy gained possession of the schooner, which was built at Hodgdon Brothers Shipyard in East Boothbay in 1921

Descriptions of 30 islands that are currently for sale in Maine, ranging in pric

Descriptions of 30 islands that are currently for sale in Maine, ranging in price from $149,000 for Pine Island in Dover-Foxcroft to$5.75 million for Davis Island at the end of the St. George Peninsula

Campylobacter jejuni strain discrimination and temperature-dependent glycome expression profiling by lectin microarray

Gram-negative Campylobacter jejuni is the leading cause of bacterial gastroenteritis in humans worldwide and the most frequently identified infectious trigger in patients developing Guillain-Barré syndrome (GBS). While C. jejuni is pathogenic in humans, it is a commensal in avian hosts. Bacterial cell surface carbohydrates are important virulence factors and play roles in adherence, colonisation and infection. The mechanisms leading to infection or persistent colonisation of C. jejuni are not well understood but host temperature may provide an important stimulus for specific adaptation. Thus, examination of the modulation of the total surface glycome of C. jejuni in response to temperature may help shed light on commensal and pathogenic mechanisms for this species. C. jejuni strains 81116 and 81-176 were cultured at 37 and 42°C to simulate human and avian host conditions, respectively, and whole cells were profiled on lectin microarrays constructed to include a wide range of binding specificities. C. jejuni 81116 profiles indicated that the previously characterised lipopolysaccharide (LPS)-like molecule and N-linked glycans were the predominantly recognised cell surface structures while capsular polysaccharide (CPS), lipooligosaccharides (LOS) and N-linked glycosylation were best recognised for strain 81-176 at 37°C. The profiles of both strains varied and were distinguishable at both temperatures. At the higher temperature, reduced dominance of the LPS-like structure was associated with strain 81116 and a change in the relative distribution of CPS and LOS structures was indicated for strain 81-176. This change in LOS molecular mass species distribution between temperatures was confirmed by SDS-PAGE analysis. Additionally, opposite behaviour of certain lectins was noted between the plate agglutination assay and the microarray platform. Insights into the important glycosylation involved in C. jejuni host cell tropism at different growth temperatures were gained using the lectin microarray platform.Funding was provided by Science Foundation Ireland (grant no. 08/SRC/B1393) in support of the Alimentary Glycoscience Research Cluster (AGRC) and the European Union FP7 programme (grant no. 260600) in support of the GlycoHIT project. MT’s Postgraduate Fellowship was funded by the College of Science and the Thomas Crawford Hayes award, NUI Galway.peer-reviewe

Campylobacter jejuni strain discrimination and temperature-dependent glycome expression profiling by lectin microarray

Gram-negative Campylobacter jejuni is the leading cause of bacterial gastroenteritis in humans worldwide and the most frequently identified infectious trigger in patients developing Guillain-Barré syndrome (GBS). While C. jejuni is pathogenic in humans, it is a commensal in avian hosts. Bacterial cell surface carbohydrates are important virulence factors and play roles in adherence, colonisation and infection. The mechanisms leading to infection or persistent colonisation of C. jejuni are not well understood but host temperature may provide an important stimulus for specific adaptation. Thus, examination of the modulation of the total surface glycome of C. jejuni in response to temperature may help shed light on commensal and pathogenic mechanisms for this species. C. jejuni strains 81116 and 81-176 were cultured at 37 and 42°C to simulate human and avian host conditions, respectively, and whole cells were profiled on lectin microarrays constructed to include a wide range of binding specificities. C. jejuni 81116 profiles indicated that the previously characterised lipopolysaccharide (LPS)-like molecule and N-linked glycans were the predominantly recognised cell surface structures while capsular polysaccharide (CPS), lipooligosaccharides (LOS) and N-linked glycosylation were best recognised for strain 81-176 at 37°C. The profiles of both strains varied and were distinguishable at both temperatures. At the higher temperature, reduced dominance of the LPS-like structure was associated with strain 81116 and a change in the relative distribution of CPS and LOS structures was indicated for strain 81-176. This change in LOS molecular mass species distribution between temperatures was confirmed by SDS-PAGE analysis. Additionally, opposite behaviour of certain lectins was noted between the plate agglutination assay and the microarray platform. Insights into the important glycosylation involved in C. jejuni host cell tropism at different growth temperatures were gained using the lectin microarray platform.Funding was provided by Science Foundation Ireland (grant no. 08/SRC/B1393) in support of the Alimentary Glycoscience Research Cluster (AGRC) and the European Union FP7 programme (grant no. 260600) in support of the GlycoHIT project. MT’s Postgraduate Fellowship was funded by the College of Science and the Thomas Crawford Hayes award, NUI Galway