250 research outputs found
Stem Cell Research and Applications: Monitoring the Frontiers of Biomedical Research
A report regarding human stem cell research was placed online in November 1999. From the American Association for the Advancement of Science (AAAS) and the Institute for Civil Society (ICS), this report explores the scientific and ethical issues involved in the use of embryonic stem (ES) cells and embryonic germ (EG) cells in medical research. While the report acknowledges the "varied social, political, ethical, and religious viewpoints to be considered in discussions about the scientific use of tissue from human embryos and fetuses," it concludes that human stem cell research holds enormous potential and advocates both private and public funding of such projects. Users can read the full text of the report, which includes specific suggestions and guidelines for the future of stem cell research, in .pdf format. The main AAAS stem cell site offers a summary of the findings and recommendations and several bioethics-related resources
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Detection of protein kinase homologues and viral RNA-binding domains utilizing polyclonal antiserum prepared against a baculovirus-expressed ds RNA-activated 68,000-Da protein kinase
The P68 protein kinase (referred to as P68 based on its
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r of 68,000 in human cells) is a serine/threonine kinase induced by interferon treatment and activated by dsRNAs. The kinase is under tight controls in virus-infected cells since once activated, it phosphorylates its natural substrate eukaryotic initiation factor 2 (elF-2), leading to potential limitations in functional elF-2 and decreases in protein synthesis initiation. To further delineate the molecular mechanisms underlying kinase regulation, we attempted to express the P68 protein kinase in insect cells using a baculovirus vector. Repeated efforts to isolate recombinant baculoviruses containing a wild-type kinase failed, whereas recombinants expressing a nonfunctional kinase with a catalytic domain II mutation were readily isolated. When used to infect Spodoptera frugiperda cells, the recombinant virus expressed the exogenous mutant protein at almost 5–10% of the total proteins synthesized. We then purified the kinase by immunoaffinity chromatography to raise monospecific antiserum which recognized not only the human native wild-type P68, but also kinase homologues in murine, bovine, and monkey cells as determined by immunoblot and immunoprecipitation analysis. Fortunately, kinase function also could be assayed using this antibody since the human and nonhuman kinase homologues, present in immunoprecipitates, were autophosphorylated and phosphorylated the natural substrate, elF-2 alpha. Further, this antiserum recognized epitopes throughout the molecule including the amino and carboxyl termini in contrast to the available monoclonal antibody.
In vitro assays using the polyclonal antibody revealed the importance of the amino terminus, especially amino acids 1–97, in the binding of the kinase to viral RNA activators and inhibitors. Finally, we determined that the P68 amino terminus was both necessary and sufficient for binding dsRNA as we were able to transfer dsRNA-binding properties to a reporter gene product previously unable to bind RNA
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