1,003 research outputs found
Lateral-Pressure Profiles in Cholesterol-DPPC Bilayers
By means of atomistic molecular dynamics simulations, we study
cholesterol-DPPC (dipalmitoyl phosphatidylcholine) bilayers of different
composition, from pure DPPC bilayers to a 1:1 mixture of DPPC and cholesterol.
The lateral-pressure profiles through the bilayers are computed and separated
into contributions from the different components. We find that the pressure
inside the bilayer changes qualitatively for cholesterol concentrations of
about 20% or higher. The pressure profile then turns from a rather flat shape
into an alternating sequence of regions with large positive and negative
lateral pressure. The changes in the lateral-pressure profile are so
characteristic that specific interaction between cholesterol and molecules such
as membrane proteins mediated solely via the lateral-pressure profile might
become possible
Systematic comparison of force fields for microscopic simulations of NaCl in aqueous solutions: Diffusion, free energy of hydration and structural properties
In this paper we compare different force fields that are widely used
(Gromacs, Charmm-22/x-Plor, Charmm-27, Amber-1999, OPLS-AA) in biophysical
simulations containing aqueous NaCl. We show that the uncertainties of the
microscopic parameters of, in particular, sodium and, to a lesser extent,
chloride translate into large differences in the computed radial-distribution
functions. This uncertainty reflects the incomplete experimental knowledge of
the structural properties of ionic aqueous solutions at finite molarity.We
discuss possible implications on the computation of potential of mean force and
effective potentials.Comment: Revised and extended manuscrip
Stability of charge inversion, Thomson problem and application to electrophoresis
We analyse charge inversion in colloidal systems at zero temperature using
stability concepts, and connect this to the classical Thomson problem of
arranging electrons on sphere. We show that for a finite microion charge, the
globally stable, lowest energy state of the complex formed by the colloid and
the oppositely charged microions is always overcharged. This effect disappears
in the continuous limit. Additionally, a layer of at least twice as many
microions as required for charge neutrality is always locally stable. In an
applied external electric field the stability of the microion cloud is reduced.
Finally, this approach is applied to a system of two colloids at low but finite
temperature
Cationic DMPC/DMTAP Lipid Bilayers: Molecular Dynamics Study
Cationic lipid membranes are known to form compact complexes with DNA and to
be effective as gene delivery agents both in vitro and in vivo. Here we employ
molecular dynamics simulations for a detailed atomistic study of lipid bilayers
consisting of a mixture of cationic dimyristoyltrimethylammonium propane
(DMTAP) and zwitterionic dimyristoylphosphatidylcholine (DMPC). Our main
objective is to examine how the composition of the bilayers affects their
structural and electrostatic properties in the liquid-crystalline phase. By
varying the mole fraction of DMTAP, we have found that the area per lipid has a
pronounced non-monotonic dependence on the DMTAP concentration, with a minimum
around the point of equimolar mixture. We show that this behavior has an
electrostatic origin and is driven by the interplay between positively charged
TAP headgroups and the zwitterionic PC heads. This interplay leads to
considerable re-orientation of PC headgroups for an increasing DMTAP
concentration, and gives rise to major changes in the electrostatic properties
of the lipid bilayer, including a significant increase of total dipole
potential across the bilayer and prominent changes in the ordering of water in
the vicinity of the membrane. Moreover, chloride counter-ions are bound mostly
to PC nitrogens implying stronger screening of PC heads by Cl ions compared to
TAP head groups. The implications of these findings are briefly discussed
Coarse-Grained Model for Phospholipid/Cholesterol Bilayer
We construct a coarse-grained (CG) model for dipalmitoylphosphatidylcholine
(DPPC)/cholesterol bilayers and apply it to large-scale simulation studies of
lipid membranes. Our CG model is a two-dimensional representation of the
membrane, where the individual lipid and sterol molecules are described by
point-like particles. The effective intermolecular interactions used in the
model are systematically derived from detailed atomic-scale molecular dynamics
simulations using the Inverse Monte Carlo technique, which guarantees that the
radial distribution properties of the CG model are consistent with those given
by the corresponding atomistic system. We find that the coarse-grained model
for the DPPC/cholesterol bilayer is substantially more efficient than atomistic
models, providing a speed-up of approximately eight orders of magnitude. The
results are in favor of formation of cholesterol-rich and cholesterol-poor
domains at intermediate cholesterol concentrations, in agreement with the
experimental phase diagram of the system. We also explore the limits of the
novel coarse-grained model, and discuss the general validity and applicability
of the present approach
Structural Effects of Small Molecules on Phospholipid Bilayers Investigated by Molecular Simulations
We summarize and compare recent Molecular Dynamics simulations on the
interactions of dipalmitoylphosphatidylcholine (DPPC) bilayers in the liquid
crystalline phase with a number of small molecules including trehalose, a
disaccharide of glucose, alcohols, and dimethylsulfoxide (DMSO). The sugar
molecules tend to stabilize the structure of the bilayer as they bridge
adjacent lipid headgroups. They do not strongly change the structure of the
bilayer. Alcohols and DMSO destabilize the bilayer as they increase its area
per molecule in the bilayer plane and decrease the order parameter. Alcohols
have a stronger detrimental effect than DMSO. The observables which we compare
are the area per molecule in the plane of the bilayer, the membrane thickness,
and the NMR order parameter of DPPC hydrocarbon tails. The area per molecule
and the order parameter are very well correlated whereas the bilayer thickness
is not necessarily correlated with them.Comment: 8 pages, 3 figures, accepted to Fluid Phase Equilibri
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