Effect of beef sire expected progeny difference for carcass conformation on live animal muscularity scores and ultrasonic muscle and fat depths, and on carcass classification and composition of their progeny

peer-reviewedThe objective was to examine the effect of sire expected progeny difference (EPD) for carcass conformation score on the live animal and carcass traits of their progeny. In each of 4 years a Charolais sire of high and one of average EPD for carcass conformation score were mated to spring-calving suckler cows and the bull and heifer progeny were taken to slaughter at 455 (s.d. 25.2) and 607 (s.d. 29.5) days of age in 4 and 3 years, respectively. The difference in EPD between the sire EPD groups for carcass conformation and fat scores (scale 1 to 15), and carcass weight were, 0.45 units, −0.53 units and 9.7 kg, respectively. Muscularity scores were recorded at weaning (7 to 9 months of age) and pre-slaughter, and ultrasound measurements were recorded pre-slaughter. Carcass weight, and conformation and fat scores were recorded at slaughter and an 8-rib pistola from the right side of each carcass was dissected into lean, fat and bone. There was no significant effect of sire EPD group on live weight or carcass weight, but kill-out proportion, ultrasound muscle depth and the Irish Cattle Breeding Federation muscularity scores were greater (P < 0.001) for progeny of the high than the average EPD group. Bull progeny of high EPD sires had better (P < 0.001) Signet muscularity scores and carcass conformation scores than bull progeny from average EPD sires, whereas there was no effect of sire EPD group on heifer progeny. Compared to progeny of the average EPD sire group, those from the high EPD group had a lower weight of kidney and channel fat (P 0.06) and carcass fat score (P < 0.05), lower proportions of fat (P < 0.001) and bone (P < 0.01) in the pistola, and higher weight of pistola, both absolutely (P < 0.01) and relative to carcass weight (P < 0.05), higher proportions of lean and high-value cuts in the pistola and higher carcass value (P < 0.001). Linear regression analysis showed that a 1 unit increase in sire EPD for carcass conformation score increased (P < 0.01) carcass lean proportion by 19.4 g/kg. In conclusion, although sire EPD for carcass conformation score was reflected in the conformation score of intensively-reared bull progeny and not in extensively-reared heifer progeny, carcass lean proportion and carcass value were higher for both genders

Post-weaning performance and carcass characteristics of steer progency from different suckler cow breed types

peer-reviewedIn two experiments a total of 44 steer progeny of spring-calving Charolais (C) and Hereford × Friesian (HF) suckler cows and C sires were slaughtered at approximately 2 years of age. Following weaning they were offered silage and 1 kg of concentrate per head daily during a 5 month winter after which they spent 7 months at pasture. In Experiment 1, animals were given a silage/concentrate diet during a finishing period of either 95 or 152 days. In Experiment 2, steers were offered either a daily diet of silage plus 6 kg of concentrates or concentrates to appetite plus 5 kg of silage (fresh weight) during the final 140-day finishing period. Following slaughter, an 8-rib pistola from each animal was dissected. For the two experiments combined C and HF progeny had carcass weights of 372 and 385 (s.e. 6.1) kg, proportions of carcass as pistola of 467 and 454 (s.e. 2.8) g/kg and pistola meat proportions of 676 and 642 (s.e. 5.1) g/kg, respectively. All fat traits were lower for the C than HF progeny but there was no difference in carcass conformation score. Increasing slaughter weight increased carcass weight (P < 0.001), kidney plus channel fat weight (P < 0.001), and pistola fat proportion (P < 0.001) and decreased the proportions of carcass as pistola (P < 0.05), pistola meat (P < 0.01), and bone (P < 0.05). In conclusion, breed type had no effect on carcass growth but the C progeny had higher meat yield than the HF. Increasing slaughter weight increased fatness and reduced meat yield

Effect of concentrate feeding level in winter and turnout date to pasture in spring on biological and economical performance of weanling cattle in suckler beef production systems

peer-reviewedThree experiments were carried out to determine the effects of supplementary concentrate feeding level (Low, LC; High, HC) to grass silage and/or turnout date to pasture in spring (Early, ET; Late, LT) for a second grazing season on performance to slaughter of spring-born, weaned beef calves (n = 188). Experiment 1 comprised of two concentrate levels (0.5 and 1.5 kg/day). Experiment 2 comprised of two turnout dates (19 March, 9 April). Experiment 3 comprised of two concentrate levels (0.5 kg and 2.0 kg/day) and two turnout dates (22 March, 12 April). In Experiment 1, live-weight gain during the indoor winter period was 25 kg higher (P 0.05) total live-weight gain for both treatments. In Experiment 2, live weight at turnout to pasture was 11 kg lower (P 0.05), subsequently. In Experiments 1 and 2, live-weight gain during the finishing period and carcass weight, conformation and fat scores did not differ (P > 0.05) between the treatments. In Experiment 3, at turnout to pasture, HC were 35 kg heavier (P < 0.001) than LC, and ET were 12 kg lighter (P < 0.05) than LT, whereas 8 days after late turnout, ET were 13 kg heavier (P < 0.05) than LT. There was a concentrate level × turnout date interaction (P < 0.05) for live weight at the end of the grazing season, whereby the LC, LT treatment were lighter than the other treatments, which did not differ. Live weight at slaughter and carcass weight did not differ (P > 0.05) between the concentrate levels, whereas they were higher (P < 0.05) for ET than LT. Economic and stochastic analysis of Experiment 3 indicated that, in the context of whole-farm systems, (i) feeding HC was dependent on date of sale such that only where progeny were sold at the start of the second grazing season, net farm margin (NFM) was increased, (ii) ET only increased NFM where progeny were retained through to finish and, (iii) taking progeny through to finish was more profitable than selling earlier in the animals’ lifetime. In conclusion, subsequent compensatory growth at pasture diminishes the growth and economic advantage from concentrate supplementation or early turnout to pasture, of young late-maturing cattle

Design and Humoral Analysis of Two Epitope-Based Brucella abortus DNA Vaccines

Brucella is a genus of Gram negative, facultative intracellular pathogens which cause brucellosis, one of the most wide spread zoonotic diseases. Brucellosis causes a severe economic burden due to reproductive loss in animals and human infection. Vaccination of animals has proven to be the most effective means of controlling brucellosis; however the current live-attenuated vaccines are not considered ideal. The live-attenuated vaccines Brucella abortus Strain 19 and RB51 remain pathogenic to humans, and the former interferes with diagnostic tests due to induction of antibodies against the O-polysaccharide. DNA vaccination with single antigens has proven to be successful at protecting mice against B. abortus challenge, however this method is less effective in large animals. Immunization with a combination of antigens has been shown to provide more protection than single antigens. In an attempt to develop a better DNA vaccine, two multivalent multi-epitope plasmids were constructed using known protective antigens and bioinformatics technologies. Epitopes predicted to induce cell-mediated immunity were selected from Cu/Zn superoxide dismutase, outer membrane protein (Omp) 16, Omp19, ribosomal subunit L7/L12, BP26, ribosomal subunit L9, and Omp25. The plasmids were transfected into Chinese hamster ovary (CHO K1) cells, and PCR was used to confirm presence of the sequences in the genome. The transcription of the BabV1 and BabV2 genes to RNA was confirmed using RT-PCR. Finally, Western blots using sera from Strain 19 infected goats suggest the protein is not recognized by the humoral response of vaccinated animals. Further research is required to determine if the p425/BabV1 and p425/BabV2 vaccines are recognized by the cell-mediated immune response of infected or vaccinated animals

Effect of age and nutrient restriction pre partum on beef suckler cow serum immunoglobulin concentrations, colostrum yield, composition and immunoglobulin concentration and immune status of their progeny

peer-reviewedThe effect of cow age (multiparous (MP) v. primiparous (PP)) and nutritional restriction pre partum (grass silage ad libitum v. straw only ad libitum for the last 15 (s.d. 3.3) days of gestation) on cow serum immunoglobulin (Ig) concentration, on colostrum yield, composition and Ig concentration and on calf serum Ig concentrations (at ~8 and 48 h post partum) using spring-calving Limousin Holstein-Friesian cows and their progeny was studied over 3 years. The method of colostrum administration (stomach tube vs. assisted suckling within 1 h post partum) on calf immune status was also investigated. When feeding colostrum the target was to give each calf 50 mL per kg birthweight via stomach tube. Colostrum yield and Ig concentration were measured following administration of oxytocin and hand-milking of half (Experiments 1 and 2) or the complete udder (Experiment 3). Following an 8-h period after birth during which suckling was prevented a further colostrum sample was obtained. There was no significant difference in first milking colostrum Ig subclass concentrations between the within-quarter fractions or between the front and rear quarters of the udder in either MP or PP cows. Colostrum Ig subclass concentrations at second milking were 0.46 to 0.65 of that at first milking. Compared to MP cows offered silage, colostrum yield and the mass of colostrum IgG1, IgG2, IgM, IgA and total Ig produced was lower (P < 0.001) for PP cows and the mass of IgG1, IgM and total Ig produced was lower (P < 0.05) for MP cows offered straw. Calves from PP cows and MP cows offered straw had significantly lower serum IgG1 and total Ig concentrations at 48 h post partum than calves from MP cows offered silage but there was no difference (P > 0.05) between colostrum feeding methods. In conclusion, calves from PP cows and MP cows offered straw had a lower humoral immune status than those from MP cows offered grass silage

The effect of cereal type and feeding frequency on intake, rumen fermentation, digestibility, growth and carcass traits of finishing steers offered a grass silage-based diet

peer-reviewedThe effect of concentrate cereal type (rolled barley-based v. rolled wheat-based) and concentrate feeding frequency (one 6 kg feed v. two 3 kg feeds per day) on intake, rumen fermentation, diet digestibility and performance of finishing steers offered grass silage to appetite was evaluated over four experiments using a total of 154 animals. Not all four feeding treatments were used in each of the four experiments. The duration of the growth measurement period was 152, 112, 111 and 113 days for experiments 1 to 4, respectively, after which all animals were slaughtered. Dietary dry matter (DM) intake and in vivo digestibility, final live weight, kill-out proportion, carcass weight, carcass conformation score, carcass fat score and daily liveweight and estimated carcass gain were not affected (P > 0.05) by cereal type or feeding frequency. Cereal type or feeding frequency had no effect (P > 0.05) on feed conversion efficiency (FCE) expressed as either live-weight or carcass gain per unit DM intake. Neither mean rumen fluid pH or concentrations of ammonia or L-lactate were influenced by cereal type or feeding frequency. The mean molar proportion of propionate was higher and that of butyrate lower (P < 0.05) with wheat than with barley. Estimated carcass weight gain and FCE to carcass were similar for wheat based and barley-based concentrate as a supplement to grass silage offered either as one feed or two equal feeds daily

Effect of suckler cow genotype on cow serum immunoglobulin (Ig) levels, colostrum yield, composition and Ig concentration and subsequent immune status of their progeny

peer-reviewedSurvival of the neonatal calf is largely dependent on humoral immunity. The objective of three experiments reported here was to compare cow serum immunoglobulin (Ig) concentration, colostrum yield, composition and Ig concentration and calf serum Ig concentrations at ~8- and 48-h post partum of spring-calving Charolais (C) and Beef × Holstein-Friesian (BF) cows and their progeny. Cows were individually offered a restricted allowance of grass silage pre partum in Experiments 1 and 2 and silage ad libitum in Experiment 3. In Experiment 1 calves were assisted to suckle after parturition. In Experiments 2 and 3, colostrum yield and Ig concentration were measured following administration of oxytocin and hand milking of half or the complete udder, respectively. It was intended to feed each calf 50 ml (Experiment 2) or 40 ml (Experiment 3) of colostrum per 1 kg birth weight via stomach tube. Following an 8-h period, during which suckling was prevented, a further colostrum sample was obtained. The decrease in cow serum IgG1 concentration pre partum was greater (P < 0.05) in BF cows than C cows. In comparison to BF cows, C cows had a lower colostrum yield (P < 0.001) and the colostrum had lower concentrations of dry matter (P < 0.01), crude protein (P < 0.05), fat (P < 0.05), IgG1 (P = 0.06), IgG2 (P < 0.01), IgM (P < 0.01) and Ig total (P < 0.05). The mass of IgG1, IgG2, IgM, IgA and Ig total in the colostrum produced was significantly lower for C cows than BF cows. Calves from C cows had significantly lower serum Ig subclass concentration at 48-h post partum than calves from BF cows. In conclusion, due to a lower Ig mass produced by their dams, calves from C cows had a lower humoral immune status than those from BF cowsTeagasc Walsh Fellowship Programm

Morning Featured Speaker — Current State of Military and Civilian Pilot Pipelines

Discussion will focus on the status of the U.S. military and civilian pipelines as of the summer 2017. Specifically, the presentation will cover the current pilot flows within the RAND pilot pipeline model. Additionally, the presentation will discuss: 1) the future plans for military pilot production, 2) forecasts for the military pilot flow into the major airlines, 3) forecasts for future major airline hiring, and 4) predicted subsequent effects on the pilot pipeline based on historical data

Current State of Military and Civilian Pilot Pipelines

Discussion will focus on the status of the U.S. military and civilian pipelines as of the summer 2017. Specifically, the presentation will cover the current pilot flows within the RAND pilot pipeline model. Additionally, the presentation will discuss: 1) the future plans for military pilot production, 2) forecasts for the military pilot flow into the major airlines, 3) forecasts for future major airline hiring, and 4) predicted subsequent effects on the pilot pipeline based on historical data