3,318 research outputs found

    Believers Armor: How the Christian Church in the United States can Survive and Overcome Spiritual Warfare

    Full text link
    This research seeks to discover why the Christian church in the United States has struggled to withstand the effects of spiritual warfare. Resisting Satan’s evil schemes continues to be a personal need and spiritual challenge facing people in today’s fastpaced digital world. Failing to resist Satan creates a severe problem for the Christian church. As a result, believers are unprepared to both understand and withstand the effects of spiritual warfare. The author, inspired by the Holy Spirit, created an artifact that has become a powerful ministry tool to address this challenge. The Armor of God challenge coin was fashioned from the Apostle Paul’s Armor of God doctrine in Ephesians, which sets the biblical foundation for writing this dissertation. The author’s primary focus is to help Christians become more aware of spiritual warfare and the critical importance of the Armor of God in winning the battles. The thesis of this research intends to show how putting on Christ prepares believers for spiritual warfare. Putting on Christ as spiritual armor is a scriptural precept and a recommended Pauline solution that helps Christians both understand and withstand the devastating effects of demonic schemes, sexual temptations, and worldly enticements. Putting on Christ is an intentional choice of will and a personal act of faith and obedience that is a metaphorical and spiritual activity. The expected outcomes from this investigation are to advance historical knowledge, increase post-modern awareness, and improve future ministry applications. These outcomes will help the Christian church in the United States survive and overcome the wiles of the devil. This study promotes contextually appropriate and biblically honoring methods to wear Christ as a personalized defense system. Standing firm in a spiritual skirmish line and advancing the Gospel of Christ one step at a time is the paper’s goal to overcoming evil principalities and powers. Helping prepare, train, and equip ministry leaders in the Pauline art of spiritual warfare creates positive outcomes. The resulting opportunities for the Christian church in the United States include reclaiming its incarnational witness, restoring trust in the pulpit, preparing the body of Christ for spiritual warfare, and advancing the Kingdom of God

    USING THE EMBL-EBI CLUSTAL OMEGA TOOL TO CALCULATE DIVERSITY OF HEAVY CHAIN PHAGE-DISPLAY LIBRARIES

    Get PDF
    Here we show that traditional Sanger sequencing combined with analysis tools available from the European Molecular Biology Laboratory-European Bioinformatics Institute (EMBL-EBI), specifically EMBOSS Transeq and Clustal Omega, is extremely effective in the analysis of naïve phage display antibody libraries for the determination of library size and diversity. The free tools are easy to use and require little manipulation of reads by hand, allowing analysis to be performed on a standard personal computer. Utilization of this technique has applicability to researchers with limited access to deep sequencing. The primary drawback to this analysis methodology is that antibodies with particular molecular or binding properties, desirable or otherwise, are not identifiable by their sequences

    Does a Carbonatite Deposit Influence Its Surrounding Ecosystem?

    Get PDF
    Carbonatites are unusual alkaline rocks with diverse compositions. Although previous work has characterized the effects these rocks have on soils and plants, little is known about their impacts on local ecosystems. Using a deposit within the Great Lakes–St. Lawrence forest in northern Ontario, Canada, we investigated the effect of a carbonatite on soil chemistry and on the structure of plant and soil microbial communities. This was done using a vegetation survey conducted above and around the deposit, with corresponding soil samples collected for determining soil nutrient composition and for assessing microbial community structure using 16S/ITS Illumina Mi-Seq sequencing. In some soils above the deposit a soil chemical signature of the carbonatite was found, with the most important effect being an increase in soil pH compared with the non-deposit soils. Both plants and microorganisms responded to the altered soil chemistry: the plant communities present in carbonatite-impacted soils were dominated by ruderal species, and although differences in microbial communities across the surveyed areas were not obvious, the abundances of specific bacteria and fungi were reduced in response to the carbonatite. Overall, the deposit seems to have created microenvironments of relatively basic soil in an otherwise acidic forest soil. This study demonstrates for the first time how carbonatites can alter ecosystems in situ

    Active learning of the thermodynamics-dynamics tradeoff in protein condensates

    Full text link
    Phase-separated biomolecular condensates exhibit a wide range of dynamical properties, which depend on the sequences of the constituent proteins and RNAs. However, it is unclear to what extent condensate dynamics can be tuned without also changing the thermodynamic properties that govern phase separation. Using coarse-grained simulations of intrinsically disordered proteins, we show that the dynamics and thermodynamics of homopolymer condensates are strongly correlated, with increased condensate stability being coincident with low mobilities and high viscosities. We then apply an "active learning" strategy to identify heteropolymer sequences that break this correlation. This data-driven approach and accompanying analysis reveal how heterogeneous amino-acid compositions and non-uniform sequence patterning map to a range of independently tunable dynamical and thermodynamic properties of biomolecular condensates

    Lessons from the TAPS study - Errors relating to medical records

    Get PDF
    The Threats to Australian Patient Safety (TAPS) Study collected 648 anonymous reports about threats to patient safety from a representative random sample of Australian general practitioners. These contained any events the GPs felt should not have happened and would not want to happen again, regardless of who was at fault or the outcome of the event. This series of articles presents clinical lessons resulting from the TAPS study.2 page(s

    Enhancing Cation Diffusion and Suppressing Anion Diffusion via Lewis-Acidic Polymer Electrolytes

    Get PDF
    Solid polymer electrolytes (SPEs) have the potential to increase both the energy density and stability of lithium-based batteries, but low Li^+ conductivity remains a barrier to technological viability. SPEs are designed to maximize Li^+ diffusivity relative to the anion while maintaining sufficient salt solubility. It is thus remarkable that poly(ethylene oxide) (PEO), the most widely used SPE, exhibits Li^+ diffusivity that is an order of magnitude smaller than that of typical counterions at moderate salt concentrations. We show that Lewis-basic polymers like PEO favor slow cation and rapid anion diffusion, while this relationship can be reversed in Lewis-acidic polymers. Using molecular dynamics, polyboranes are identified that achieve up to 10-fold increases in Li^+ diffusivities and significant decreases in anion diffusivities, relative to PEO in the dilute-ion regime. These results illustrate a general principle for increasing Li^+ diffusivity and transference number with chemistries that exhibit weaker cation and stronger anion coordination

    Lessons from the TAPS study - Managing investigation results - is your practice system safe?

    Get PDF
    The TAPS study found that errors in the process of providing health care were reported by general practitioners more than twice as often as deficiencies in a clinician's knowledge or skills. Approximately 20% of these process error events concerned investigations. In addition, some reported events that related to investigations included filing system and recall errors, which accounted for a further 10% of reported error events.2 page(s

    Efficient Activation of Reconstructed Rat Embryos by Cyclin-Dependent Kinase Inhibitors

    Get PDF
    This Article is brought to you for free and open access by the Molecular and Cellular Biochemistry at UKnowledge. It has been accepted for inclusion in Molecular and Cellular Biochemistry Faculty Publication by an authorized administrator of UKnowledge. For more information, please contac
    • …
    corecore