Effects of verbenalin on prostatitis mouse model

AbstractThe aim of this study was to observe the treatment characteristics of verbenalin on a prostatitis mouse model. Give Xiaozhiling injection in the prostate locally to make a prostatitis mouse model. High, medium and low doses of verbenalin were each given to different mouse groups. The amount of water was determined in 14th, 28th. The number of white cells and lecithin corpuscle density in prostatic fluid were determined. Morphological changes in the prostate, testis, epididymis and kidney were detected. Compared with the model control group, the mice treated with high, medium and low doses of verbenalin had significantly increased amounts of water, and prostate white blood cell count and prostate volume density (Vv) were decreased significantly, the density of lecithin corpuscle score increased, and pathologic prostatitis changes were significantly reduced. Pathological change in the testis was significantly reduced and the change in the epididymis was obviously reduced. The thymic cortex thickness and the number of lymphocytes increased significantly and could reduce the renal pathological changes in potential. Verbenalin has a good therapeutic effect on the prostatitis mouse model

Synthesis of dental resins using diatomite and nano-sized SiO2 and TiO2

AbstractThe mechanical properties of dental composites were improved by porous diatomite and nano-sized silica (OX-50) used as co-fillers. The resin composites, filled with silanized OX-50 and silanized diatomite (40:60wt/wt), presented the best flexural strength (133.1MPa), elastic modulus (9.5GPa) and Vickers microhardness (104.0HV). Besides these, TiO2 nanoparticles were introduced to tune the dental resin composites colours which were valued by the CIE-Lab system. The colour parameters (L⁎, a⁎, b⁎) showed that the colour changes of resin composites could be perceived obviously, when 300–400nm TiO2 particles were introduced as fillers. The resin composite, filled with 0.5wt% TiO2, exhibited both clear discolouration (ΔE⁎=3.22) and high mechanical strength. Using scanning electron microscope (SEM) equipped with an energy dispersive X-ray (EDX), the titanium elemental mapping results indicated that the TiO2 particles were distributed evenly in the prepared dental composites

A pH-sensitive multifunctional gene carrier assembled via layer-by-layer technique for efficient gene delivery

Peng Li, Donghua Liu, Lei Miao, Chunxi Liu, Xiaoli Sun, Yongjun Liu, Na ZhangSchool of Pharmaceutical Science, Shandong University, Jinan, Shandong, People’s Republic of ChinaBackground: The success of gene therapy asks for the development of multifunctional vectors that could overcome various gene delivery barriers, such as the cell membrane, endosomal membrane, and nuclear membrane. Layer-by-layer technique is an efficient method with easy operation which can be used for the assembly of multifunctional gene carriers. This work describes a pH-sensitive multifunctional gene vector that offered long circulation property but avoided the inhibition of tumor cellular uptake of gene carriers associated with the use of polyethylene glycol.Methods: Deoxyribonucleic acid (DNA) was firstly condensed with protamine into a cationic core which was used as assembly template. Then, additional layers of anionic DNA, cationic liposomes, and o-carboxymethyl-chitosan (CMCS) were alternately adsorbed onto the template via layer-by-layer technique and finally the multifunctional vector called CMCS-cationic liposome-coated DNA/protamine/DNA complexes (CLDPD) was constructed. For in vitro test, the cytotoxicity and transfection investigation was carried out on HepG2 cell line. For in vivo evaluation, CMCS-CLDPD was intratumorally injected into tumor-bearing mice and the tumor cells were isolated for fluorescence determination of transfection efficiency.Results: CMCS-CLDPD had ellipsoidal shapes and showed “core-shell” structure which showed stabilization property in serum and effective protection of DNA from nuclease degradation. In vitro and in vivo transfection results demonstrated that CMCS-CLDPD had pH-sensitivity and the outermost layer of CMCS fell off in the tumor tissue, which could not only protect CMCS-CLDPD from serum interaction but also enhance gene transfection efficiency.Conclusion: These results demonstrated that multifunctional CMCS-CLDPD had pH-sensitivity, which may provide a new approach for the antitumor gene delivery.Keywords: layer-by-layer, multifunctional nanovector, pH-sensitivity, gene deliver

Adrenomedullin expression in epithelial ovarian cancers and promotes HO8910 cell migration associated with upregulating integrin α5β1 and phosphorylating FAK and paxillin

<p>Abstract</p> <p>Background</p> <p>Epithelial ovarian cancer (EOC) is one of the leading causes of cancer deaths in women worldwide. Adrenomedullin (AM) is a multifunctional peptide which presents in various kinds of tumors.</p> <p>Methods</p> <p>In this study, we characterized the expression and function of AM in epithelial ovarian cancer using immunohistochemistry staining. Exogenous AM and small interfering RNA (siRNA) specific for AM receptor CRLR were treated to EOC cell line HO8910. Wound healing assay and flow cytometry were used to measure the migration ability and expression of integrin α5 of HO8910 cells after above treatments. Western blot was used to examine the phosphorylation of FAK and paxillin.</p> <p>Results</p> <p>We found that patients with high AM expression showed a higher incidence of metastasis, larger residual size of tumors after cytoreduction and shorter disease-free and overall survival time. Exogenous AM induced ovarian cancer cell migration in time- and dose- dependent manners. AM upregulated the expression of integrin α5 and phosphorylation of FAK, paxillin as well.</p> <p>Conclusions</p> <p>Our results suggested that AM contributed to the progression of EOC and had additional roles in EOC cell migration by activating the integrin α5β1 signaling pathway. Therefore, we presumed that AM could be a potential molecular therapeutic target for ovarian carcinoma.</p

SpotServe: Serving Generative Large Language Models on Preemptible Instances

The high computational and memory requirements of generative large language models (LLMs) make it challenging to serve them cheaply. This paper aims to reduce the monetary cost for serving LLMs by leveraging preemptible GPU instances on modern clouds, which offer accesses to spare GPUs at a much cheaper price than regular instances but may be preempted by the cloud at any time. Serving LLMs on preemptible instances requires addressing challenges induced by frequent instance preemptions and the necessity of migrating instances to handle these preemptions. This paper presents SpotServe, the first distributed LLM serving system on preemptible instances. Several key techniques in SpotServe realize fast and reliable serving of generative LLMs on cheap preemptible instances. First, SpotServe dynamically adapts the LLM parallelization configuration for dynamic instance availability and fluctuating workload, while balancing the trade-off among the overall throughput, inference latency and monetary costs. Second, to minimize the cost of migrating instances for dynamic reparallelization, the task of migrating instances is formulated as a bipartite graph matching problem, which uses the Kuhn-Munkres algorithm to identify an optimal migration plan that minimizes communications. Finally, to take advantage of the grace period offered by modern clouds, we introduce stateful inference recovery, a new inference mechanism that commits inference progress at a much finer granularity and allows SpotServe to cheaply resume inference upon preemption. We evaluate on real spot instance preemption traces and various popular LLMs and show that SpotServe can reduce the P99 tail latency by 2.4 - 9.1x compared with the best existing LLM serving systems. We also show that SpotServe can leverage the price advantage of preemptive instances, saving 54% monetary cost compared with only using on-demand instances.Comment: ASPLOS 202

(E)-N-{(E)-2-[(3,5-Dimethylbiphenyl-4-yl)imino]­acenaphthen-1-yl­idene}-2,6-di­methyl-4-phenyl­aniline

The title compound, C40H32N2, has crystallographic twofold rotation symmetry, with two C atoms lying on the axis. The dihedral angle between the two benzene rings of the 4-phenyl-2,6-dimethyl­phenyl group is 35.74 (17)°. The acenaphthene ring makes an angle of 76.93 (11)° with the benzene ring bonded to the N atom and an angle of 41.53 (13)° with the other benzene ring

Impact of V-ets Erythroblastosis Virus E26 Oncogene Homolog 1 Gene Polymorphisms Upon Susceptibility to Autoimmune Diseases

V-ets erythroblastosis virus E26 oncogene homolog 1 (ETS1) is recognized as a gene of risk to autoimmune diseases (ADs). Two single nucleotide polymorphisms (SNPs) in ETS1 (rs1128334 G\u3eA and rs10893872 T\u3eC) were considered associated with ADs risk. However, the results remain conflicting. We performed a meta-analysis to evaluate more precise estimations of any relationship. We searched PubMed, OvidSP, and Chinese National Knowledge Infrastructure databases (papers published prior to September 12, 2014) and extracted data from eligible studies. Meta-analysis was performed using the STATA 12.0 software. Random effect model or fixed effect model were chosen according to the study heterogeneities. A total of 11 studies including 7359 cases (9660 controls) for rs1128334 and 8 studies including 5419 cases (7122 controls) for rs10893872 were involved in this meta-analysis. Overall, our results showed that there were significant associations for rs1128334 with AD risk in 5 genetic models, both in pooled analysis and in systemic lupus erythematous (SLE) subgroup, and in 3 genetic models of the uveitis subgroup. Although for rs10893872, the results showed that there were significant associations in allele model both in pooled analysis and in SLE subgroup. As a conclusion, this meta-analysis demonstrated that these 2 SNPs (rs1128334 and rs10893872) in ETS1were associated with ADs risk