EXPERIMENTAL STUDIES OF SOLUBILITY OF ELEMENTAL SULPHUR IN SUPERCRITICAL CARBON DIOXIDE BETWEEN 10 AND 40 MPA

International audienc

Experimental Studies of solubility of Elemental Sulphur in Supercritical Nitrogen

International audienc

Adult cardiac myocytes survive and remain excitable during long-term culture on synthetic supports

AbstractObjective: Cardiomyocytes can be transplanted successfully into skeletal and cardiac muscle. Our goal was to determine the feasibility of grafting cardiomyocytes onto various synthetic supports to create an excitable and viable tissue for implantation. Methods: Adult rat cardiomyocytes were cultured over an 8-week period onto different substitutes, including human glutaraldehyde-treated pericardium (n = 3), equine glutaraldehyde-treated pericardium (n = 3), polytetrafluoroethylene (n = 8), Dacron polyester (n = 16), and Vicryl polyglactin (n = 8). Results: Only the cells seeded on the Dacron survived, with the synthetic fibers colonized at 8 weeks. On the other supports, the number of myocytes progressively decreased from the first week, with their density (number of cells per square millimeter) being, after 20 days, 17 ± 2 on the polytetrafluoroethylene and 5 ± 1 on the human or equine pericardium compared with 45 ± 3 on the Dacron. After 8 weeks of culture on Dacron, the sarcomeric protein (sarcomeric α-actinin) was detected in all cells. In addition, the staining was regularly arranged and well aligned in a striated pattern. Spontaneous beating activity was obtained. Moreover, electrical stimulation of the cell preparation resulted in the generation of calcium transients, the frequency of which followed the frequency of the electrical stimulation. Conclusions: These results suggest that adult cardiac myocytes remain viable and excitable during long-term culture on a 3-dimensional Dacron support, which might constitute a new synthetic cardiac tissue. (J Thorac Cardiovasc Surg 2001;121:510-9

OPTIMISATION DU TRANSFERT DE GENES AUX MYOCYTES VENTRICULAIRES ADULTES ET EFFETS PHYSIOLOGIQUES DU TRANSFERT DE SERCA

PARIS5-BU Saints-Pères (751062109) / SudocSudocFranceF

Développement préclinique de la thérapie génique du myocarde (optimisation de la délivrance in vivo et application à la surexpression d' une Ca2+-ATPase du réticulum sarcosplasmique)

PARIS7-Bibliothèque centrale (751132105) / SudocSudocFranceF

Implications des Ca2+-ATPases du réticulum sarcoplasmique et du sarcolemme dans le modèle de l'infarctus du myocarde chez le rat

PARIS7-Bibliothèque centrale (751132105) / SudocSudocFranceF

Les cytokines proinflammatoires dans l'insuffisance cardiaque (application à un modèle de culture cellulaire in vitro et perspectives thérapeutiques)

PARIS7-Bibliothèque centrale (751132105) / SudocSudocFranceF