Molecular cloning, sequence, structural analysis and expression of the histidyl-tRNA synthetase gene from Streptococcus equisimilis.

The histidyl-tRNA synthetase gene (hisS) from Streptococcus equisimilis was cloned and sequenced. The gene for this aminoacyl-tRNA synthetase has an open reading frame of 1278 nucleotides. The deduced amino acid sequence encodes a protein of 426 amino acids with MW = 47,932. The protein is predicted to be soluble with a pl = 5.27. The protein sequence has extensive overall identity/similarity with the Escherichia coli and the yeast histidyl-tRNA synthetases (approximately 58% and approximately 20%, respectively). A putative promoter for gene transcription lies within two hundred nucleotides of the polypeptide start codon. The enzyme was overexpressed, to a level of about 18% of total cellular protein, as a fusion protein (containing an additional 15 amino acids) in E. coli using the pT7 expression system containing the T7 RNA polymerase/promoter (Tabor and Richardson, Proc. Natl. Acad. Sci. U.S.A. 82:1074-1078, 1985). The predicted MW for the hisS gene product is in good agreement with the size of the fusion protein determined by SDS-PAGE (M(r) = 53,700). Amino acid sequencing of the intact fusion protein and proteolytic fragments confirmed the deduced sequence of the synthetase at many positions throughout the protein. The expressed protein catalyzed the specific aminoacylation of tRNA(His) in vitro

Work-Nonwork Spillover of Wage Justice through Work Capabilities in Low and Middle Income Workers

Wage justice literature asserts that it is the perceived fairness of pay (based on comparison others) instead of actual pay that matters most to an employee. This study therefore investigates the spillover effect of wage justice on life capabilities in the Philippines. It is hypothesized that wage justice will influence life capability through work capabilities such as job empowerment, job satisfaction, and occupational pride. Using data from the Institute of Philippine Culture study on living wages of 500 individuals, a structural equation model was estimated to test the spillover effect. Findings confirms the hypothesis that wage justice influences life capabilities (life satisfaction and physical well-being) through work capabilities. Implications for research on wage justice and capabilities as well as to people management practices in work organizations are discussed

Sequence-tagged sites and low-cost DNA marker technology for rice

Sequence-tagged sites (STSs) facilitate the conversion of a genetic map into a physical map, provide a common basis for the comparison of diverse types of mapping data, are stored and disseminated as electronic data, and are amplified from genomic DNA by polymerase chain reaction (PCR). STSs find application as DNA markers in breeding programs and germplasm management because they offer speed, convenience, reliability, and low cost in genomic analysis, but these applications are currently limited by the small number of STSs available. We report here the terminal sequencing of 354 DNA markers of the Cornell-IRRI genetic map of rice and the conversion of 100 of them into STSs by synthesis of pairs of PCR primers. PCR was used to amplify the corresponding loci from genomic DNA of IR36 (indica), Taichung 65 (japonica), and Oryza longistaminata (AA genome wild species). More than half of the RZ clones amplified DNA segments that were 0.1-2.0 kbp larger than expected, presumably because of the presence of introns. Amplicon length polymorphisms were detected between O. sativa and O. longistaminata for about one quarter of the clones. The applications of STSs are illustrated by reference to 1) DNA marker-aided selection for pyramiding of bacterial blight resistance genes, 2) breeding for gall midge resistance, 3) monitoring the inheritance of transgenes, and 4) analysis of genetic variation of AA genome wild species