Symptomatic Abdominal Simple Cysts: Is Percutaneous Sclerotherapy with Hypertonic Saline and Bleomycin a Treatment Option?

Aim. To evaluate the feasibility of percutaneous sclerotherapy of symptomatic simple abdominal cysts, using hypertonic saline and bleomycin, as an alternative to surgery. Materials and Methods. This study involved fourteen consecutive patients (ten women, four men, mean age: 59.2 y) with nineteen symptomatic simple cysts (liver n=14, kidney n=3, and adrenal n=2) treated percutaneously using a modified method. Initially CT-guided drainage was performed; the next day the integrity of the cyst/exclusion of extravasation or communications was evaluated under fluoroscopy, followed by two injections/reabsorptions of the same quantity of hypertonic NaCl 15% solution and three-time repetition of the same procedure with the addition of bleomycin. The catheter was then removed; the patients were hospitalized for 12 hours and underwent follow-ups on 1st, 3rd, 6th, and 12th months. Cyst’s volumes and the reduction rate (%) were calculated in each evaluation. Results. No pain or complications were noted. A significant cyst’s volume reduction was documented over time (P<0.001). On the 12th month 17 cysts disappeared and two displayed a 98.7% and 68.9% reduction, respectively. Conclusion. This percutaneous approach constitutes a very promising nonsurgical alternative for patients with symptomatic simple cyst, without complications under proper precautions, leading to eliminating the majority of cysts

Fertilization Affects Severity of Disease Caused by Fungal Plant Pathogens

Commercial fertilizers are commonly applied in farming to maximize crop yield. Lifting nutrient limitation to plant growth when water and light conditions are sufficient may permit plants to grow to the maximum of their ability; however, plant ability to resist pathogen infections is also modified. A meta-analysis was conducted on 57 articles to identify the way plant disease severity of fungal pathogen-induced infection is modified following fertilization, and the key regulators of such an effect. The analysis largely focused on N fertilization events in order to minimize the effect of heterogeneity that could result from differences in the way different nutrient fertilizers are able to modify plant disease severity. Fungal pathogen identity and fungal pathogen lifestyle were the main significant regulators affecting the extent of the modification of plant disease resistance following N fertilization, whereas contradictory results were obtained with the susceptibility of plant species. No differences were detected between pot or field experiments and following artificial or natural infection. Although in the vast majority of instances N fertilization increased disease severity, characteristic plant species and fungal pathogens could be identified for which disease severity following N fertilization declined. It is concluded that the potential of some plant species such as Solanum spp. to show reduced disease severity following N fertilization requires further investigation, as in such cases N fertilization could potentially be used as an additional means of suppressing fungal pathogens

The Use of Spatial Interpolation to Improve the Quality of Corn Silage Data in Case of Presence of Extreme or Missing Values

Agricultural spatial analysis has the potential to offer new ways of analyzing crop data considering the spatial information of the measurements. Moving from farmers&rsquo; estimates and crop-cuts techniques to interpolation is a new challenge, and a promising path to achieving more reliable results, especially in the case of field data with extreme or missing values. By comparing the main descriptive statistics of three types of crop parameters (fresh weight, dry weight, and ear weight) in three randomly taken maize plots, we found that the issue of missing values can be addressed by using interpolation to calculate estimated values of given parameters in non-sampling locations. Moreover, based on the descriptive statistics, the implementation of interpolation can reduce crop field variability (extreme values) and achieve an improvement of coefficient of variation (CV) values up to 30%, compared with other methods used, such as the replacing of missing values by the average of all data, or the average of the row or column, with an improvement of only up to 15%. These findings strongly suggest that the implementation of an interpolation method in case of extreme or missing values in crop data is an effective process for improving their quality, and consequently, their reliability. As a result, the application of spatial interpolation to existing crop data can provide more dependable estimations of average crop parameters values, compared to the usual farmers&rsquo; estimates

Path Planning for Autonomous Robotic Platform based on Created Sampling Maps

Soil properties are of great importance in crop management, as they highly affect plant growth, crop production and product quality. In order to examine these properties, soil samples must be collected from the entire surface of the field. An effective soil sampling requires careful selection of the total number and the location of the samples. Therefore, soil properties can present heterogeneity along the field. For that reason, distributing sampling points evenly along the field is not considered as a best practice. In this research, in order to define the location of sampling points, the field was divided into homogenous management zones based on electrical conductivity (ECa) values. An equal number of points was distributed in each zone and a sampling map was created. Subsequently, a path for autonomous navigation was generated based on the created sampling map. More specifically, points of the map were distributed in the shortest possible distance order for the robotic platform to move while collecting the samples. In order to test the accuracy of the path planning, the proposed path was uploaded to the robotic platform and the movement was mapped. The path that was followed by the robotic platform was quite similar to the simulated path. The results of this research suggest that sensors such as a penetrometer can be mounted on an autonomous robotic platform in order to collect data from sampling points by moving along the created path. © 2022 Copyright for this paper by its authors. Use permitted under Creative Commons License Attribution 4.0 International (CC BY 4.0

Multiple resistance of silky windgrass to acetolactate synthase-and acetyl-CoA synthase-inhibiting herbicides

Field and pot experiments were conducted in Greece to study the occurrence of resistance in silky windgrass to acetolactate synthase (ALS)-and acetyl-CoA synthase (ACCase)-inhibiting herbicides. Twenty-four populations of silky windgrass were examined in whole-plant response experiments. High levels of field-evolved resistance to chlorsulfuron (0% to 28% control in terms of fresh weight reduction) with the recommended field rates were confirmed in most silky windgrass populations. However, other ALS inhibitors, such as pyroxsulam and a premix of mesosulfuron-methyl and iodosulfuron, provided adequate control (76% to 100% in terms of fresh weight reduction) of most populations, except eight silky windgrass populations that were found to be cross-resistant to all ALS-inhibiting herbicides tested (i.e., chlorsulfuron, commercial mixture of mesosulfuron-methyl plus iodosulfuron, and pyroxsulam). Conversely, most silky windgrass populations were controlled effectively (90% to 100% in terms of fresh weight reduction) with the recommended field rates of ACCase inhibitors cycloxydim, clethodim, and pinoxaden, but five populations were also found to be resistant to clodinafop-propargyl (10% to 68% control in terms of fresh weight reduction). The ALS gene sequencing of the eight silky windgrass populations, with cross-resistance to ALS inhibitors, revealed a point mutation at the Pro-197 position, causing amino acid substitution by Ser or Thr in the ALS enzyme. Overall, chlorsulfuron and clodinafop-propargyl were selecting agents of field-evolved multiple resistance to ALS-and ACCase-inhibiting herbicides in five silky windgrass populations. As the available postemergence-applied chemistries/modes of action registered for grass weed control in cereals are rather limited, adopting integrated management practices and implementing proactive and reactive measures to delay the evolution of resistant populations is essential.

Dose-response and growth rate variation among glyphosate resistant and susceptible Conyza albida and Conyza bonariensis populations

Plant responses to glyphosate applied at different doses were examined for one glyphosate resistant (R) and one glyphosate susceptible (S) population of Conyza albida and C. bonarienis. Growth rates and development stages of five R C. albida and three R C. bonarienis populations were also compared with those of their respective S counterparts to investigate the possible impact of the glyphosate resistance trait on their fitness. The GR50 values for C. albida R (3.94−5.22 kg a.i. · ha−1) and S (0.24−0.31 kg a.i. · ha−1) populations were higher than those of C. bonariensis R (0.60−1.51 kg a.i. · ha−1) and S (0.10−0.13 kg a.i. · ha−1). The growth rate (slope b) of one R C. albida population was lower than the respective S and other R populations, while growth rates of most R and S C. bonariensis populations were similar. Some R populations showed inconsistent differences in some development stages when compared to those of the S ones, which cannot be attributed to the glyphosate resistance trait

Development of a simple and low-resource regeneration system of two greek tomato varieties

Native tomato landrace varieties (Solanum lycopersicum L.) are sources of high genetic diversity and are adaptable to local environmental conditions. The in vitro propagation system can be used as a tool to produce disease-free, high-quality propagation material and preserve the unique characteristics of commercial Greek tomato varieties, such as the variety Areti and the traditional landrace variety Makedonia, both of exceptional quality and economic importance. Twenty- and 27-day-old cotyledon and leaf explants were cultivated in 4 regeneration media supplemented with 0 or 0.1 mg∙L−1 indole-3-acetic acid and 0.5 or 1 mg∙L−1 zeatin. Regeneration efficiency was dependent on variety, explant type and age, and regeneration media. Areti was a more appropriate target genetic material, exhibiting a six-fold greater response to regeneration compared to Makedonia, regardless of media and explant age. The regenerated shoots of both varieties were successfully rooted (60%) and acclimatized (98%). This regeneration protocol would be valuable in the production of propagation material for commercial and conservation practices and in breeding programs for genetic improvement. © 2021 by the authors. Licensee MDPI, Basel, Switzerland

Framework of Methodology to Assess the Link between A Posteriori Dietary Patterns and Nutritional Adequacy: Application to Pregnancy

This study aimed to explore the nutritional profile of 608 women during the second trimester of pregnancy, in terms of nutrient patterns, dietary quality and nutritional adequacy. Dietary data were collected using a validated Mediterranean-oriented, culture-specific FFQ. Principal component analysis was performed on 18 energy-adjusted nutrients. Two main nutrient patterns, “plant-origin” (PLO) and “animal-origin” (ANO), were extracted. Six homogenous clusters (C) relative to nutrient patterns were obtained and analyzed through a multidimensional methodological approach. C1, C5 and C6 scored positively on PLO, while C1, C2 and C3 scored positively on ANO. When dietary quality was mapped on food choices and dietary indexes, C6 unveiled a group with a distinct image resembling the Mediterranean-type diet (MedDiet Score = 33.8). Although C1–C5 shared common dietary characteristics, their diet quality differed as reflected in the HEI-2010 (C1:79.7; C2:73.3; C3:70.9; C4:63.2; C5:76.6). The appraisal of nutritional adequacy mirrored a “nutritional-quality gradient”. A total of 50% of participants in C6 had almost 100% adequate magnesium intake, while 50% of participants in C4 had a probability of adequacy of ≤10%. Our methodological framework is efficient for assessing the link between a posteriori dietary patterns and nutritional adequacy during pregnancy. Given that macro- and micronutrient distributions may induce metabolic modifications of potential relevance to offspring’s health, public health strategies should be implemented

Maternal Dietary Protein Patterns and Neonatal Anthropometrics: A Prospective Study with Insights from NMR Metabolomics in Amniotic Fluid

This study aimed to characterize dietary protein patterns (DPPs) in a sample pool of 298 well-nourished pregnant women and explore potential associations between DPPs and neonatal anthropometrics. Maternal dietary data were collected using a validated food frequency questionnaire. Neonatal anthropometrics were abstracted from health booklets. A hierarchical cluster analysis identified three DPPs: “Dairy-focused”, “Med-fusion”, and “Traditional-inspired”. The “Dairy-focused” DPP exhibited the highest protein intake (p p p p p p p = 0.007) and the lowest ponderal index (p = 0.003). The NMR-metabolomics approach was implemented on a subset of women that provided amniotic fluid (AF) specimens (n = 62) to elucidate distinct metabolic signatures associated with DPPs. PCA and OPLS-DA models verified the adherence to three DPPs, revealing that the levels of several amino acids (AAs) were the highest in “Dairy-focused”, reflecting its protein-rich nature. The “Traditional-inspired” DPP showed decreased AAs and glucose levels. This knowledge may contribute to optimizing maternal dietary recommendations. Further research is needed to validate these findings and better understand the relationships between maternal diet, AF metabolic signature, and neonatal anthropometrics