Fat composition assessment by 1H and 13C spectroscopy in mice

Proceeding of: 17th Scientific Meeting, International Society for Magnetic Resonance in Medicine, 18-24 April, 2009, Honolulu, Hawai, US

Quantitative comparison of partial fourier reconstruction algorithms in MRI at 7T

[Poster] 4th European Molecular Imaging Meeting, Barcelona, Spain, May 27 - 30, 2009In this work we present a quantitative comparison at 7T of the most common partial fourier reconstruction algorithms: conjugate synthesis with phase correction, Margosian method, homodyne reconstruction, PoCS algorithm and iterative homodyne reconstructionThis work is supported in part by the projects CdTeaM (CeniT-ingenio 2010), Ministerio de Ciencia e innovación, and Ciber Cb07/09/0031 CiberSaM, Ministerio de Sanidad y Consumo.Publicad

Reduction of respiratory blurring in small-animal CT scans based on a fast retrospective gating method

[Abstract] The 22nd International Congress and Exhibition, Barcelona, Spain, June 25-28, 2008The purpose of the present work was to develop a fast retrospective method to extract the respiratory signal from the CT projections in cone beam geometry and to obtain dynamic breathing studies in small animal scans. The whole process had to be software-based and automatic, avoiding the use of any additional respiratory gating instrumentsPublicad

Multimodal assessment of myocardial infarction in rats: comparison of late gadolinium enhanced MRI and PET

[Poster] 4th European Molecular Imaging Meeting, Barcelona, Spain, May 27 - 30, 2009Myocardial infarction (Mi) size in rats has been assessed using Mri and nuclear imaging, but little information is available on the suitability and assessment of the information provided by each technique. We are running a study to compare results on the infarct size as assessed by each modality 30 days after an induced Mi in ratsThis work is supported by the reCaVa-reTiC network, Ministerio de Ciencia e innovación (TeC2008-06715-C02-01 and TeC2007-64731/TCM) and Ministerio de industria (CdTeaM, Programa CeniT)Publicad

Molecular physiopathology of obesity-related diseases: multi-organ integration by GRK2

Obesity is a worldwide problem that has reached epidemic proportions both in developed and developing countries. The excessive accumulation of fat poses a risk to health since it favours the development of metabolic alterations including insulin resistance and tissue inflammation, which further contribute to the progress of the complex pathological scenario observed in the obese. In this review we put together the different outcomes of fat accumulation and insulin resistance in the main insulin-responsive tissues, and discuss the role of some of the key molecular routes that control disease progression both in an organ-specific and also in a more systemic manner. Particularly, we focus on the importance of studying the integrated regulation of different organs and pathways that contribute to the global pathophysiology of this condition with a specific emphasis on the role of emerging key molecular nodes such as the G protein-coupled receptor kinase 2 (GRK2) signalling hubMinisterio Sanidad y Consumo-Instituto de Salud Carlos III, Spain; Grants SAF2014-55511-R and SAF2012-36400 from Ministerio de Economia y Competitividad (MINECO), Spain (to FM-CM and MS); S2010/BMD-2332 (INDISNET) from Comunidad de Madrid, Spain (to FM); an EFSDNovo Nordisk Grant (to FM) and Fundacion Ramon Areces (to CM and AMB)Peer Reviewe

Validation of a restrospective respiratory gating method for small-animal CT scanners

Proceeding of: 2008 IEEE Nuclear Science Symposium Conference Record (NSS '08), Dresden, Germany, 19-25 October 2008We propose a retrospective respiratory gating algorithm to generate dynamic CT studies. To this end, we compared three different methods of extracting the respiratory signal from the projections of small-animal cone-beam computed tomography (CBCT) scanners. Given a set of frames acquired from a certain axial angle, subtraction of their average image from each individual frame produces a set of difference images. The respiratory signals were extracted by analyzing the shape of the histogram of these difference images: we calculated the first four central and non-central moments. However, only odd-order moments produced the desired breathing signal, as the even-order moments lacked information about the phase. Each of these curves was compared to a reference signal recorded by means of a pneumatic pillow. Given the similar correlation coefficients yielded by all of them, we selected the mean to implement our retrospective protocol. Respiratory phase bins were separated, reconstructed independently and included in a dynamic sequence, suitable for cine playback. We validated our method in 5 adult rat studies by comparing profiles drawn across the diaphragm dome, with and without retrospective respiratory gating. Results showed a sharper transition in the gated reconstruction, with an average slope improvement of 60.7%.This project was supported by the CENIT Programme (Ministerio de Industria), CIBER CB07/09/0031 and RETICRECAVA (Ministerio de Sanidad y Consumo), and TEC2007-64731 TCM (Ministerio de Educaci6n y Ciencia)

Uso del método de Split Bregman para la resolución del problema de compressed sensing en imagen de resonancia magnética dinámica cardiaca para pequeño animal

Actas de: XXIX Congreso Anual de la Sociedad Espñaola de Ingeniería Biomédica (CASEIB 2011). Cáceres, 16-18 Noviembre 2011.La imagen dinámica de resonancia magnética en pequeño animal es una herramienta muy importante en el estudio de enfermedades cardiovasculares. La reducción de los tiempos de adquisición de este tipo de imágenes es especialmente relevante para la obtención de imágenes de calidad con una buena resolución espacial y temporal. Actualmente existen diversas técnicas de aceleración que permiten reducir estos tiempos de adquisición, entre ellas la técnica de 'compressed sensing', en auge en los últimos años. Ésta técnica permite la reconstrucción de una imagen a partir de datos submuestreados mediante el uso de métodos de reconstrucción no lineales que minimizan la variación total de la imagen. Recientemente el método de Split Bregman ha demostrado ser computacionalmente eficiente para resolver este problema en imágenes de resonancia magnética. En este trabajo se amplía la metodología de Split Bregman para minimizar la variación total espacial y temporal en imágenes dinámicas, y se aplica a imágenes cardiacas de pequeño animal. Los resultados preliminares muestran que con la metodología propuesta es posible reducir el tiempo de adquisición hasta 5 veces manteniendo la calidad de imagen.Este trabajo ha sido financiado parcialmente por el Ministerio de Ciencia e Innovación (Red RECAVA) y la Comunidad de Madrid y los Fondos FEDER (proyecto ARTEMIS-S2009DPI-1802)Publicad

Phenolic profile changes of grapevine leaves infected with Erysiphe necator

Powdery mildew in grapevine is caused by Erysiphe necator and its control requires many chemical treatments. Numerous efforts are being made to improve disease management to achieve crop sustainability goals. The exogenous induction of plant immune responses is one of the most encouraging strategies currently being developed. The objective of this research was to analyse differences in phenolic compound concentrations in E. necator-infected leaves of two varieties of Vitis vinifera, Tempranillo and Tempranillo Blanco, using ultra performance liquid chromatography coupled with mass spectrometry. To understand the susceptibility of the varieties, in vitro assays using whole leaves were done.This work was funded by the Spanish Ministry of Economy and Competitivity (Ministerio de Economía y Competitividad, MINECO) under project BIO2017-86375-R.Peer reviewe

Validation of rat brain MR image intensity non-uniformity correction using surface coil images

[Poster] 4th European Molecular Imaging Meeting, Barcelona, Spain, May 27 - 30, 2009Non-uniform intensity artifacts confound the quantitative analysis of magnetic resonance (Mr) images of animal studies, particularly when using surface coils and high-field magnets. The use of correction methods proposed and validated on human brain images such as the n3 algorithm has previously been reported only on mouse images acquired with a volume coil. here, we evaluate the performance of n3 specifically on Mr rat brain images acquired with a surface coilCdTeaM (CeniT-ingenio 2010), Ministerio de Ciencia e innovación, Ciber Cb07/09/0031 CiberSaM, Ministerio de Sanidad y ConsumoPublicad

Quantification of SPIO using T2* and phase imaging

Proceeding of: 17th Scientific Meeting, International Society for Magnetic Resonance in Medicine, 18-24 April, 2009, Honolulu, Hawai, USAPublicad