Health and development of children born after assisted reproductive technology and sub-fertility compared to naturally conceived children: data from a national study

In a non-matched case-control study using data from two large national cohort studies, we investigated whether indicators of child health and development up to 7 years of age differ between children conceived using assisted reproductive technology (ART), children born after sub-fertility (more than 24 months of trying for conception) and other children. Information on ART use/sub-fertility was available for 23,649 children. There were 227 cases (children conceived through ART) and two control groups: 783 children born to sub-fertile couples, and 22,639 children born to couples with no fertility issues. In models adjusted for social and demographic factors there were significant differences between groups in rate of hospital admissions before the children were 9 months old (P=0.029), with the ART group showing higher rates of hospital admission than the no fertility issues control group, the sub-fertile control group being intermediate between the two. Children born after ART had comparable health and development beyond 9 months of age to their naturally conceived peers. This applied to the whole sample and to a sub-sample of children from deprived neighborhoods

The health and development of children born to older mothers in the United Kingdom: observational study using longitudinal cohort data

Objective: To assess relations between children’s health and development and maternal age. Design: Observational study of longitudinal cohorts. Setting: Millennium Cohort Study (a random sample of UK children) and the National Evaluation of Sure Start study (a random sample of children in deprived areas in England), 2001 to 2007. Participants: 31 257 children at age 9 months, 24 781 children at age 3 years, and 22 504 at age 5 years. Main outcome measures: Childhood unintentional injuries and hospital admissions (aged 9 months, 3 years, and 5 years), immunisations (aged 9 months and 3 years), body mass index, language development, and difficulties with social development (aged 3 and 5 years). Results: Associations were independent of personal and family characteristics and parity. The risk of children having unintentional injuries requiring medical attention or being admitted to hospital both declined with increasing maternal age. For example, at three years the risk of unintentional injuries declined from 36.6% for mothers aged 20 to 28.6% for mothers aged 40 and hospital admissions declined, respectively, from 27.1% to 21.6%. Immunisation rates at nine months increased with maternal age from 94.6% for mothers aged 20 to 98.1% for mothers aged 40. At three years, immunisation rates reached a maximum, at 81.3% for mothers aged 27, being lower for younger and older mothers. This was linked to rates for the combined measles, mumps, and rubella immunisation because excluding these resulted in no significant relation with maternal age. An increase in overweight children at ages 3 and 5 years associated with increasing maternal age was eliminated once maternal body mass index was included as a covariate. Language development was associated with improvements with increasing maternal age, with scores for children of mothers aged 20 being lower than those of children of mothers aged 40 by 0.21 to 0.22 standard deviations at ages 3 and 4 years. There were fewer social and emotional difficulties associated with increasing maternal age. Children of teenage mothers had more difficulties than children of mothers aged 40 (difference 0.28 SD at age 3 and 0.16 SD at age 5). Conclusion: Increasing maternal age was associated with improved health and development for children up to 5 years of age

The Tgif2 gene contains a retained intron within the coding sequence

BACKGROUND: TGIF and TGIF2 are homeodomain proteins, which act as TGFβ specific Smad transcriptional corepressors. TGIF recruits general repressors including mSin3 and CtBP. The related TGIF2 protein functions in a similar manner, but does not bind CtBP. In addition to repressing TGFβ activated gene expression, TGIF and TGIF2 repress gene expression by binding directly to DNA. TGIF and TGIF2 share two major blocks of similarity, encompassing the homeodomain, and a conserved carboxyl terminal repression domain. Here we characterize two splice variants of the Tgif2 gene from mouse and demonstrate that the Tgif2 gene contains a retained intron. RESULTS: By PCR from mouse cDNA, we identified two alternate splice forms of the Tgif2 gene. One splice variant encodes the full length 237 amino acid Tgif2, whereas the shorter form results in the removal of 39 codons from the centre of the coding region. The generation of this alternate splice form occurs with the mouse RNA, but not the human, and both splice forms are present in all mouse tissues analyzed. Human and mouse Tgif2 coding sequences contain a retained intron, which in mouse Tgif2 is removed by splicing from around 25–50% of RNAs, as assessed by RT-PCR. This splicing event is dependent on sequences within the mouse Tgif2 coding sequence. Both splice forms of mouse Tgif2 encode proteins which are active transcriptional repressors, and can repress both TGFβ dependent and independent transcription. In addition, we show that human and mouse Tgif2 interact with the transcriptional corepressor mSin3. CONCLUSION: These data demonstrate that the Tgif2 gene contains a retained intron, within the second coding exon. This retained intron is not removed from the human mRNA at a detectable level, but is spliced out in a significant proportion of mouse RNAs. This alternate splicing is dependent entirely on sequences within the mouse Tgif2 coding sequence, suggesting the presence of an exonic splicing enhancer. Both splice forms of mouse Tgif2 produce proteins which are functional transcriptional repressors

Performing against the odds: developmental trajectories of children in the EPPSE 3 to 16 study

The Effective Pre-School, Primary and Secondary Education (EPPSE 3 to 16) research project is a large scale, longitudinal, mixed-methods research that is following the progress of 3,000 children since 1997 from the age of 3- to 16-years-old. A focus for EPPSE has been the extent to which pre-school, compulsory education and children’s home learning experiences (HLE) can reduce inequality. Earlier EPPSE found that what parents did with their children was important in terms of the children’s outcomes, not simply ‘who they were’ in terms of social class and income. Following a pilot study with disadvantaged children who were ‘succeeding against the odds’ towards the end of primary school, this study provides in-depth exploration and explanation of how risks and protective factors in the lives of children shape their learning life-courses, and why they lead to academic resilience for some but not for others

Severe maternal psychopathology and infant-mother attachment.

Eighty-two mother-infant dyads, comprising women with psychiatric disorder and individually matched controls, were followed up over the children's 1st year of life. The mothers with mental illness consisted of two subgroups: first, 25 severely mentally ill mothers who had been admitted to a psychiatric unit with their infants; and second, 16 mothers from a community sample meeting research diagnostic criteria for unipolar, nonpsychotic depression. With the exception of six dyads in the in-patient group, observations were made of the mother-infant interaction and the quality of the infant-mother attachment relationship at 12 months. The nature and course of the mothers' illness was also documented. Although few residual symptoms of maternal mental illness were detected at 1 year postpartum, interactional disturbances were evident among the case group dyads. A strong association was revealed between infant-mother attachment quality and maternal diagnosis; a manic episode of illness in the postpartum period was related to security in the attachment relationship, and psychotic or nonpsychotic depression was related to insecurity. Concurrent patterns of mother-infant interaction provided support for this finding

Paternal age, paternal presence and children’s health: an observational study

In an observational study of 31,257 children we investigated the effects of paternal age at the time of the child’s birth, paternal absence and non-biological fathers on children’s health. Results are per 5 year change in paternal age. Older fathers were associated with lower rates of unintentional injuries, odds ratio (OR)=0.966, P=0.0027. There was a quadratic association between paternal age and risk of hospital admission, β=0.0121, P=0.0109, with minimum risk at paternal age37.7. Absent fathers were associated with increased risk of hospital admission, OR=1.19,P<10-3, lower rates of complete immunizations to 9 months, OR=0.562, P<10-3, higher Strength and Difficulties Questionnaire (SDQ) difficulties scores: β=0.304, P=0.0024 (3 year olds), β=0.697, P<10-3 (5 year olds). Non-biological fathers were associated with increased risk of unintentional injury, OR=1.16, P=0.0319 and hospital admission,OR=1.26, P=0.0166; lower rates of complete immunizations to 9 months, OR=0.343, P=0.0309 and higher SDQ difficulties scores:β=0.908, P<10-3

The parenting of preschool children by older mothers in the United Kingdom

The parenting literature has focussed on teenage motherhood but less is known about older mothers. In industrialises societies more women are giving birth later in life. The study examined whether there are any age trends in the use of discipline, home organisation, provision of learning opportunities, maternal responsivity and mother child relationships treating maternal age at birth as a continuous construct. The sample was from two national UK cohorts with common assessments at 3 years (N =24,610). Withholding treats or attention as discipline and parent/child conflict decreased as maternal age increased. Harsh discipline such as smacking was low for teenage mothers, highest in the mid-twenties after which it declined. Household chaos decreased with maternal age increasing up to age 30 after which it was likely to be higher. Positive and responsive parenting generally increased with maternal age up to about the age of 40 after which it plateaued. Thus overall, while older motherhood is associated with medical risks for mother and child it should not present problems in relation to parenting during the preschool years