57 research outputs found
NEURONAL ALTERATIONS IN HIPPOCAMPUS FOLLOWING SEVERE HYPOGLYCAEMIA: A LIGHT MICROSCOPIC AND ULTRASTRUCTURAL STUDY IN THE RAT
Correlation between Hypermetabolism and Neuronal Damage during Status Epilepticus Induced by Lithium and Pilocarpine in Immature and Adult Rats
EARLY CHANGES IN THE RAT HIPPOCAMPUS FOLLOWING SEIZURES INDUCED BY BICUCULLINE OR L-ALLYLGLYCINE: A LIGHT AND ELECTRON MICROSCOPE STUDY
Reduction of gaba and glutamate transporter messenger rnas in the severe-seizure genetically epilepsy-prone rat
The genetically epilepsy-prone rat is an animal model of inherited generalised tonic-clonic epilepsy that shows abnormal susceptibility to audiogenic seizures and a lowered threshold to a variety of seizure-inducing stimuli. Recent studies suggest a crucial role for glutamate and GABA transporters in epileptogenesis and seizure propagation. The present study examines the levels of expression of the messenger RNAS encoding the glial and neuronal glutamate transporters, GLT-1 and EAAC-1, and the neuronal GABA transporter, GAT-1, in paired male genetically epileptic-prone rats and Sprague-Dawley control rats using the technique of in situ hybridization. In a parallel study, semiquantitative immuno-blotting was used to assess GLT-1 and EAAC-1 protein levels in similarly paired animals. Animals were assessed for susceptibility to audiogenic seizures on six occasions, and killed seven days following the last audiogenic stimulus exposure. Rat brains were processed for in situ hybridization with radioactive35S-labelled oligonucleotide probes (EAAC-1 and GAT-1),35S-labelled riboprobes (GLT-1), and Fluorescein-labelled riboprobes (GLT-1 and GAT-1) or processed for immunoblotting using subtype-specific antibodies for GLT-1 and EAAC-1. Semiquantitative analyses were carried out on X-ray film autoradiograms in several brain regions for both in situ hybridization and immunoblotting studies. Reductions in GAT-1 messenger RNA were found in genetically epileptic-prone rats in all brain regions examined (−8 to −24% compared to control). Similar reductions in GLT-1 messenger RNA expression levels were seen in cortex, striatum, and CA1 (−8 to −12%) of genetically epileptic-prone rats; the largest reduction observed was in the inferior colliculus (−20%). There was a tendency for a reduced expression of EAAC-1 messenger RNA in most regions of the genetically epileptic-prone rat brain although this reached statistical significance only in the striatum (−12%). In contrast, no significant differences in GLT-1 and EAAC-1 protein between genetically epileptic-prone rats and control animals were observed in any region examined, although there was a tendency to follow the changes seen with the corresponding messenger RNAs. These results show differences in the messenger RNA expression levels of three crucial amino acid transporters. For the two glutamate transporters, GLT-1 and EAAC-1, differences in messenger RNA levels are not reflected or are only partially reflected in the expression of the corresponding proteins
Seizure-Related Phenomena: Serial Change of Cerebral Blood Flow and Intracranial Pressure in Status Epilepticus
The effects of L-DOPA on glutamate dehydrogenase activity in the cerebral neurons of rats with different motor activities
The Changes in Serum Prolactin Following Seizures in Children with Secondary Generalized Epilepsy
Hypoglycaemia in paediatric anaesthesia: The influence of metoclopramide and oral maltose in paediatric surgical patients
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