The Effect of Paraprotein on Platelet Aggregation

Background Some patients with paraproteinemia have platelet aggregation disorders and the aim of this study was to examine disturbance of platelet aggregation in healthy blood donors by isolated paraprotein in vitro. Methods Using Rivanol, paraprotein was separated from the serum of ten patients with paraproteinemia, who had decreased platelet aggregation with several inducers. Platelet aggregation in ten healthy donors was measured with and without addition of the isolated induced paraprotein. The test was repeated with added human immunoglobulins for intravenous use. Results Average of maximal levels of platelet aggregation has been significantly decreased in plasma rich in platelets (PRP) of healthy donors after addition of paraprotein when inducers are used: adenosine diphosphate (ADP) (P = 0.007), collagen (COL) (P = 0.008), ristocetin (RIS) (P = 0.001), and epinephrine (EPI) (P = 0.002). Average of latent time of platelet aggregation was significantly prolonged in healthy donors after addition of paraprotein with inducers: COL (P = 0.008), RIS (P = 0.008) and EPI (P = 0.006) while addition of human immunoglobulins caused no change in platelet aggregation. In comparison, when human immunoglobulins were added, maximal platelet aggregation and latent time did not change significantly. Paraprotein isolated from patients with paraproteinamia, who had decrease platelet aggregation, had significantly decreased platelet aggregation when added to PRP of healthy donors, in vitro. Conclusion Platelet aggregation was not significantly changed was confirmed with addition of human immunoglobulins

Isolation of 'mating fraction' from Chlamydomonas reinhardtii gametic flagellum membranes by affinity chromatography

Solubilized with Triton X-100 strain 137+ gametic flagellum membrane material was bound to CNBr-activated Sepharose 4B, and used for affinity chromatography of labeled with 125I 89 gametic flagellum membrane soluble in Triton X-100. A single peak containing 2.7-4.7% of total radioactivity was obtained upon pH change of the eluting buffer. Upon re-chromatography 50-70% of this material was adsorbed and eluted. The complex was found to be stable at pH 7.0-8.0 in the presence of divalent cations (Ca+2 and Mg+2)

Alkylresorcinols in rye (Secale cereale L.) grains. II. Dependence of alkylresorcinols level on weight and specific weight of grains

A relation was found between the alkylresoroinols content and the weight of single grains, their specific weight and situation in the ear. In the group of grains with high specific weight (above 1.300 g/cm3) the relative alkykesorcinols content proved to be a constant value, whereas the total content was positively correlated with the grain weight. A negative correlation was found between the specific weight and the relative alkylresorcinols content decreasing from tip to base of ear. In the apical zone of growth grains with low specific weight (below 1.260 g/cm3) constitute 70 per cent, and in the middle and base of the ear 30 per cent of all grains in the given zone