63 research outputs found
Structure and function analyses of the purified GPCR human vomeronasal type 1 receptor 1
The vomeronasal system is one of several fine-tuned scent-detecting signaling systems in
mammals. However, despite significant efforts, how these receptors detect scent remains an
enigma. One reason is the lack of sufficient purified receptors to perform detailed
biochemical, biophysical and structural analyses. Here we report the ability to express and
purify milligrams of purified, functional human vomeronasal receptor hVN1R1. Circular
dichroism showed that purified hVN1R1 had an alpha-helical structure, similar to that of
other GPCRs. Microscale thermophoresis showed that hVN1R1 bound its known ligand myrtenal
with an EC50 ∼1 µM. This expression system can enable structural and functional
analyses towards understanding how mammalian scent detection works
Arabic prosody and its applications in Muslim poetry. Edited By Lars Johanson and Bo Utas. (Transactions, Vol. 5.) pp. 145. Istanbul, Swedish Research Institute, 1994. Distributed by Almqvist and Wiksell International. SEK 152.
Decreases in Pheromonal Responses at the Accessory Olfactory Bulb of Mice with a Deficiency of the α1B or β3 Subunits of Voltage-Dependent Ca2+-Channels
Effect of Ocular Enucleation and Altered Lighting Regimens at Various Ages on Adrenal and Gonadal Weight and Mean Plasma Corticosteroid Levels in the Rat
Developmental Hormonal Profiles in rdw Rats with Congenital Hypothyroidism Accompanying Increased Testicular Size and Infertility in Adulthood
Thyroid Hormones and Testis Development: A Model System for Increasing Testis Growth and Sperm Production
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