22 research outputs found
Syncope in patients with drug-resistant epilepsy without apparent cardiovascular disease
Clotrimazole Preferentially Inhibits Human Breast Cancer Cell Proliferation, Viability and Glycolysis
BACKGROUND: Clotrimazole is an azole derivative with promising anti-cancer effects. This drug interferes with the activity of glycolytic enzymes altering their cellular distribution and inhibiting their activities. The aim of the present study was to analyze the effects of clotrimazole on the growth pattern of breast cancer cells correlating with their metabolic profiles. METHODOLOGY/PRINCIPAL FINDINGS: Three cell lines derived from human breast tissue (MCF10A, MCF-7 and MDA-MB-231) that present increasingly aggressive profiles were used. Clotrimazole induces a dose-dependent decrease in glucose uptake in all three cell lines, with K(i) values of 114.3±11.7, 77.1±7.8 and 37.8±4.2 µM for MCF10A, MCF-7 and MDA-MB-231, respectively. Furthermore, the drug also decreases intracellular ATP content and inhibits the major glycolytic enzymes, hexokinase, phosphofructokinase-1 and pyruvate kinase, especially in the highly metastatic cell line, MDA-MB-231. In this last cell lineage, clotrimazole attenuates the robust migratory response, an effect that is progressively attenuated in MCF-7 and MCF10A, respectively. Moreover, clotrimazole reduces the viability of breast cancer cells, which is more pronounced on MDA-MB-231. CONCLUSIONS/SIGNIFICANCE: Clotrimazole presents deleterious effects on two human breast cancer cell lines metabolism, growth and migration, where the most aggressive cell line is more affected by the drug. Moreover, clotrimazole presents little or no effect on a non-tumor human breast cell line. These results suggest, at least for these three cell lines studied, that the more aggressive the cell is the more effective clotrimazole is
AbsenteÃsmo-doença entre profissionais de enfermagem da rede básica do SUS Campinas
Crude ethanol extract from babassu (Orbignya speciosa): cytotoxicity on tumoral and non-tumoral cell lines
Efeitos auditivos da exposição combinada: interação entre monóxido de carbono, ruÃdo e tabagismo
Influence of chloride contamination on carbonation of cement-based materials
An experimental investigation was conducted to understand the effect of the presence of chloride on the carbonation front. Microstructural analysis was conducted to verify the carbonation development in material previously contaminated by chlorides. Accelerated tests were combined to understand how the presence of chlorides influences carbonation front and how chloride test method influences in these results. The results indicate that the presence of chloride decreases the carbonation front in cementbased
materials. This behaviour is related to the pore refinement and humidity retention caused by chloride. The impact on carbonation reduction occurs at different levels, according to the testing method used