A Rare Renal Epithelial Tumor: Mucinous Cystadenocarcinoma Case Report and Review of the Literature

Primary renal mucinous cystadenocarcinoma is a very rare lesion of kidney which originates from the metaplasia of the renal pelvic uroepithelium. Only one case with primary mucinous cystadenocarcinoma has been reported in the English literature. We report second case of mucinous cystadenocarcinoma which was radiologically classified as type-IIF Bosniak cyst in peripheral localization. We aimed to present this extreme and unusual entity with its radiological, surgical, and pathologic aspects under the light of literature

Genotoxic Effects of a Particular Mixture of Acetamiprid and alpha-Cypermethrin on Chromosome Aberration, Sister Chromatid Exchange, and Micronucleus Formation in Human Peripheral Blood Lymphocytes

WOS: 000275804800007PubMed ID: 19319990The genotoxic effects of a particular mixture of acetamiprid (Acm, neonicotinoid insecticide) and alpha-cypermethrin (alpha-cyp, pyrethroid insecticide) on human peripheral lymphocytes were examined in vitro by chromosomal aberrations (CAs), sister chromatid exchange (SCE), and micronucleus (MN) tests. The human peripheral lymphocytes were treated with 12.5 + 2.5, 15 + 5, 17.5 + 7.5, and 20 + 10 mu g/mL of Acm+alpha-cyp, respectively, for 24 and 48 h. The mixture of Acm + alpha-cyp induced the CAs and SCEs at all concentrations and treatment times when compared with both the control and solvent control and these increases were concentration-dependent in both treatment times. MN formation was significantly induced at 12.5 + 2.5, 15 + 5, 17.5 + 7.5, mu g/mL of Acm + alpha-cyp when compared with both controls although these increases were not concentration-dependent. Binuclear cells could not be detected sufficiently in the highest concentration of the mixture (20 + 10 mu g/mL) for both the 24- and 48-h treatment times. Mitotic index (MI), proliferation index (PI) and nuclear division index (NDI) significantly decreased because of the cytotoxic and cytostatic effects of the mixture, at all concentrations for two treatment periods. Significant decreases in MI and PI were concentration dependent at both treatment times. The decrease in NDI was also concentration-dependent at 48-h treatment period. In general, Acm + alpha-cyp inhibited nuclear division more than positive control, mitomycin C (MMC) and showed a higher cytostatic effect than MMC. Furthermore, in this article, the results of combined effects of Acm + alpha-cyp were compared with the results of single effects of Acm or alpha-cyp (Kocaman and Topaktas, 2007, 2009, respectively). In conclusion, the particular mixture of Acm + alpha-cyp synergistically induced the genotoxicity/cytotoxicity in human peripheral blood lymphocytes. (C) 2009 Wiley Periodicals, Inc. Environ Toxicol 25: 157-168, 2010.Cukurova University Research FundCukurova University [FEF2003D20]Contract grant sponsor: Cukurova University Research Fund Contract grant number: FEF2003D2

CHK2 1100delC, IVS2+1G > A and I157T mutations are not present in hepatocellular cancer cases from a Turkish population

WOS: 000313768900010PubMed ID: 23107771Aim: The cell cycle checkpoint kinase 2 (CHK2) protein participates in the DNA damage response in many cell types. Germline mutations in CHK2 (1100delC, IVS2 + 1G>A and I157T) have been associated with a range of cancer types. This study aimed to investigate whether CHK2 1100delC, IVS2 + 1G>A and I157T mutations play an important role in the development of hepatocellular carcinoma (HCC) in a Turkish population. Methods: A total of 165 hepatocellular cancer cases and 446 cancer-free controls were genotyped for CHK2 mutations by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and allele specific-polymerase chain reaction (AS-PCR) methods. Results: We did not find CHK2 1100delC, IVS2 + 1G>A and I157T mutations in any of 611 Turkish subjects. Conclusion: Our results demonstrate for the first time that CHK2 1100delC, IVS2 + 1G>A and I157T mutations have not been a genetic susceptibility factor for HCC in the Turkish population. Overall, our data suggests that genotyping of CHK2 mutations in clinical settings in the Turkish population should not be recommended. Independent studies are needed to validate our findings in a larger series, as well as in patients of different ethnic origins. (c) 2012 Elsevier B.V. All rights reserved.Cukurova University Research FundCukurova University [FEF2008D4]The authors thank all the subjects who participated in this study. We would like to thank Aynur Bekar and Ersin Akgollu for excellent technical assistance. We also thank Dr. Heli Nevanlinna, Dr. Cezary Cybulski, Dr. Natalia Bogdanova, and Dr. Borge G. Nordestgaard for having kindly provided us with the 1100delC, I157T and IVS2 + 1G>A carrier DNAs used as a positive control. We also would like to thank Mahmut Sansal for English corrections. This article is dedicated to my newborn son Kemal Can Bayram. This work was supported by the Cukurova University Research Fund FEF2008D4

CHEK2 1100delC, IVS2+1G > A and I157T mutations are not present in colorectal cancer cases from Turkish population

WOS: 000309818500018PubMed ID: 22521562Background: The cell cycle checkpoint kinase 2 (CHEK2) protein participates in the DNA damage response in many cell types. Germline mutations in CHEK2 (1100delC, IVS2+1G>A and I157T) have been impaired serine/threonine kinase activity and associated with a range of cancer types. This hospital-based case-control study aimed to investigate whether CHEK2 1100delC, IVS2+1G>A and I157T mutations play an important role in the development of colorectal cancer (CRC) in Turkish population. Methods: A total of 210 CRC cases and 446 cancer-free controls were genotyped for CHEK2 mutations by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and allele specific-polymerase chain reaction (AS-PCR) methods. Results: We did not find the CHEK2 1100delC, IVS2+1G>A and I157T mutations in any of the Turkish subjects. Conclusion: Our result demonstrate for the first time that CHEK2 1100delC, IVS2+1G>A and I157T mutations have not been agenetic susceptibility factor for CRC in the Turkish population. Overall, our data suggest that genotyping of CHEK2 mutations in clinical settings in the Turkish population should not be recommended. However, independent studies are need to validate our findings in a larger series, as well as in patients of different ethnic origins. (C) 2012 Elsevier Ltd. All rights reserved.Cukurova University Research FundCukurova University [FEF2008D4]The authors thank all the subjects who participated in this study. We also thank Dr. Heli Nevanlinna, Dr. Cezary Cybulski, Dr. Natalia Bogdanova, Dr. Borge G. Nordestgaard for having kindly provided us with the 1100delC, I157T and IVS2+1G>A carrier DNAs used as a positive control. This work was supported by Cukurova University Research Fund FEF2008D4

In vitro cytogenetic evaluation of the particular combination of flurbiprofen and roxithromycin

Flurbiprofen (FLB) (anti-inflammatory and analgesic drug) and roxithromycin (RXM) (antibiotic) were widely used in world wide. This study deals with investigation of genotoxicity, cytotoxicity, and oxidative stress effects of a particular combination of these drugs in human cultured lymphocytes. Also, DNA damaging-protective effects of combination of these drugs were analyzed on plasmid DNA. Human lymphocytes were treated with different concentrations (FLB + RXM; 10 mg/mL + 25 mg/mL, 15 mg/mL + 50 mg/mL, and 20 mg/mL + 100 mg/mL) of the drugs following by study of their genotoxic and cytotoxic effects by analysis of cytokinesisblock micronucleus test and nuclear division index, respectively. The effect of the combination in aspect of anti-oxidative and DNA damaging activity was evaluated on Pet-22b plasmid. According to our results, the combination of FLB and RXM did not show a notable genotoxic effect on cells. Although each of the substances had been shown as a cytotoxic agent by previous researchers, in this research, the combination of these drugs did not exhibit any adverse effect on cell division. FLB had DNA protection effect against H2O2 while in combination with RXM had not the same effect on the plasmid

A Rare Renal Epithelial Tumor: Mucinous Cystadenocarcinoma Case Report and Review of the Literature

Primary renal mucinous cystadenocarcinoma is a very rare lesion of kidney which originates from the metaplasia of the renal pelvic uroepithelium. Only one case with primary mucinous cystadenocarcinoma has been reported in the English literature. We report second case of mucinous cystadenocarcinoma which was radiologically classified as type-IIF Bosniak cyst in peripheral localization. We aimed to present this extreme and unusual entity with its radiological, surgical, and pathologic aspects under the light of literature