Transcription of adaptive-immune genes upon challenge with infectious pancreatic necrosis virus (IPNV) in DNA vaccinated rainbow trout

In the present study, rainbow trout weighting 3±0.3 g were vaccinated with an oral DNA vaccine encoding VP2 gene of a prevalent isolate of IPNV in Iranian trout farms encapsulated in sodium alginate microspheres and Chitosan tripolyphosphate (CS/TPP) nanoparticles. The vaccinated fish were then challenged with a virulent isolate of IPNV at 30 days post-vaccination. The transcriptional changes of adaptive- immune genes (IgM and IgT), as well as the  VP4 gene of IPNV, as an indicators of viral replication were studied 45 days post-challenge. Analysis of RT-qPCR data showed lower levels of VP4 gene expression in the oral DNA vaccinated trout after IPNV challenge compared with the control one. Moreover, the constructed DNA vaccine did not enhance the expression of IgM and IgT genes above the levels observed in the carrier control group but it showed a mimic of viral activity and contributes to maintaining them at appreciable levels in vaccinated group

Analytical method for ferroresonance solutions in series compensated power systems due to GICs: a graphical approach

Arising from solar storms, the emerging disturbances imposed on Earth's magnetic field, can drive the flow of quasi-DC geomagnetically induced currents (GICs) in power transmission systems. The ground connections of power transformers provide a closed path through which GICs flow and push their cores into half-cycle saturation. In addition, series capacitor units are often utilized to compensate HV transmission systems. The half-cycle saturation of power transformers due to GICs, on the one hand, and the proximity of such saturated transformers to capacitor compensation units on the other can lead to the inception of ferroresonance in series compensated power systems. To prevent catastrophic equipment failures due to ferroresonance during GICs, it is crucial to determine ferroresonance solutions of networks. This paper's principal contribution is to develop an approach to analyze ferroresonance in series capacitor compensated networks during GICs. This is attained by employing a simplified equivalent single-phase model on which to mount the analysis. The authenticity of this method is verified through an EMTP-RV simulation of a benchmark example power system.</p

Ovrednotenje pridelka in učinkovitosti izrabe vode kvinoje pri različnih načinih namakanja in dodatku gama aminomaslene kisline in komposta deževnikov

The current study was aim to evaluate the interaction effects of gamma aminobutyric acid (GABA) and vermicompost on yield and yield components of quinoa under different levels of drought stress. For this, two experiments were similarly designed as the factorial-split with four replicates for evaluating this hypothesis. Irrigation regimes (50, 75, and 100 % of plant water requirement (PWR)) as the main plot and vermicompost V (0, 5 t ha-1) × gamma aminobutyric acid GABA levels (0, 5, 10 mg l-1) as the subplot were designed. Severe drought stress had a significant effect on plant height. Plant height reduced 31.8 % after using 50 % of PWR compared to the control conditions. Although drought stress negatively affected the 1000 seed mass and seed yield, GABA foliar application alleviated these effects. After using 50 % of PWR, 10 mg l-1 of GABA increased the seed yield and harvest index up to 21.22 and 15.5 %, respectively, compared to the non-foliar application. The reduction in PWR from 100 to 50 % led to increasing in P and K concentrations, as well as sugar and proline contents. In the same conditions, the use of GABA or V had a significant effect on improving these traits. A similar trend was also recorded in relation to water use efficiency. Therefore, using 10 mg l-1 of GABA and 5 t ha-1 of V can be effective in alleviating water stress.Namen raziskave je bil ovrednotiti vzajemne učinke gama amino maslene kisline (GABA) in komposta deževnikov (vermikomposta) na pridelek in njegove komponente kvinoje pri različnih ravneh sušnega stresa. V ta namen sta bila izvedena dva podobna poskusa kot poskusa z deljenkami s štirimi ponovitvami. Načini namakanja (50, 75 in 100 % potrebe rastlin po vodi (PWR)) so bili na glavni ploskvi in dodatki vermikomposta (V; 0, 5 t ha-1) ter gama aminomaslene kisline (GABA; (0, 5, 10 mg l-1) na podploskvah. Velik sušni stres je imel značilen učinek na višino rastlin. Višina rastlin se je zmanjšala za 31,8 % pri 50 % oskrbi rastlin z vodo v primerjavi s kontrolo. Čeprav je sušni stres negativno vplival na maso1000 semen in pridelek semena, je foliaren nanos GABA ublažil te učinke. Pri 50 % oskrbi z vodo je dodatek 10 mg l-1 of GABA povečal pridelek semena in žeteveni indeks za 21,22 in 15,5 % v primerjavi z obravnavanjem brez foliarnega dodatka GABA. Zmanjšanje PWR iz 100 na 50 % je vodilo k povečanju koncentracij P in K, kot tudi k povečanju vsebnosti sladkorja in prolina. Pod enakimi pogoji je imela uporaba GABA ali V značilen učinek na izboljšanje teh lastnosti. Podoben trend je bil zabeležen glede povezave z učinkovitostjo izrabe vode. Zaradi vsega naštetega je uporba 10 mg l-1 GABA in 5 t ha-1 V lahko učinkovita pri blažitvi sušnega stresa

Evaluation of quantitative relationships between saffron yield and nutrition (on farm trial)

In order to relate production of saffron and utilization of nutrients, a study was conducted in 2001 and 2002. Four selected locations for this study were Birjand, Gonabad, Qaen and Torbat-Haydariah, which are the main saffron production centers in Iran. This study was performed in 160 saffron farms, aged between 1 and 5 years. Manure, nitrogen and phosphorous fertilizers showed a positive linear relation with yield and length of flowering, while nitrogen and phosphorous showed a negative linear relation with start of flowering period. Yield of saffron showed a significant and positive correlation with the amount of applied manure and the saffron farms with age 4-5 year had highest yield. Our results showed that manure was the most effective factor in production of saffron. The beneficial effects of manure could be due to slow release of nutrients and enhancing soil physical properties. Stepwise regression analysis of yield and fertilizer application showed that 67 percent of yield variations was attributed to manure and phosphorous application

HNMR-Based Metabolomics Survey in Breast Cancer Cell Line Treated by Chimera Alpha – Fetoprotein (AFP) Peptide

Abstract Purpose: Breast cancer is one of the most common cancer among. Chemotherapy and radiation along with surgery are common methods for treating cancer, but they exhibit side effects on normal cells in the body. We used peptides, as new anti-cancer agents, seem to have fewer reactions on the body's natural cells and are specialized markers for targeting cancer cells. Materials and methods: Metabolomics data are obtained by 1HNMR, LC/MS and GC/MS spectrometry and analyzed by chemometrics techniques and the affected metabolic cycles identified using different databases. ZR-75-1 cells were collected with estradiol (positive control), without estradiol (negative control) and estradiol with peptide (treatment group) and the metabolites of these 3 groups were collected by chloroform/methanol or water extraction method. Spectra were analyzed by 1HNMR and chemometric methods using PLS-DA techniques by which differentiating chemical shifts and their respective metabolites were identified using the Human Metabolome Database. The differentiating metabolic pathways were detected using Metaboanalyst.ca website. Results: A concentration of 10-9 M estradiol induced the growth of estradiol-dependent ZR-75-1 cells compared to the control group. A concentration of 10-10 M MH-I peptide inhibited the growth of estradiol-induced growth in this ER + breast cancer cell line. Altered metabolites and metabolic pathway were distinguished. Conclusion: Changes were observed in different amino acids and carbohydrates. The pathways of aminoacyl-t-RNA, glycolysis. Gluconeogenesis and biosynthesis of biotin and amino acids showed the most changes. The results from this study introduces a new peptide drug lead for the treatment of breast cancer with estrogen positive receptor.</jats:p

Innovative Solid-State Ferroresonance-Suppressing Circuit for Voltage Transformer Protection in Wind Generation Systems

Ferroresonance, as an undesirable disturbance, leads to significant overvoltage and distorted waveforms. This phenomenon can be highly damaging to voltage transformers and other parallel-connected equipment and can entail catastrophic consequences. This paper aims to design and study a solid-state ferroresonance-suppressing circuit (SSFSC) to protect voltage transformers (VTs) together with other parallel-connected equipment in wind generation systems from the adverse effects of the ferroresonance phenomenon. The proposed structure consists of low-voltage circuits, including power IGBTs. The excellent performance of the proposed SSFSC in suppressing ferroresonance overvoltage in wind generation VTs has been authenticated by analyses conducted utilizing a wind generation system model. In order to validate the performance of the proposed SSFSC, detailed analytical studies and time-domain simulations have been carried out employing a MATLAB/Simulink environment. The results verify that the proposed SSFSC can effectively suppress ferroresonance phenomena in VTs and mitigate their accompanying overvoltages with a high operational speed