Solid state fermentation: An effective fermentation strategy for the production of L-asparaginase by Fusarium culmorum (ASP-87)

Production of L-asparaginase by Fusarium culmorum (ASP-87) isolated from tropical soil was investigated under solid state fermentation on a laboratory scale using sixty five (65) agro based materials. Among the different agro based materials evaluated, soybean meal supported maximum L-asparaginase production (7.21 U/gds). Various optimization strategies for the production of L-asparaginase were also carried out with soybean meal and it was observed that inoculum size of 1 � 108 spores/mL, day 6 of incubation period, 3 mm of particle size of the substrate, moisture content of 70, initial pH of 7.0 and temperature at 30 °C were found to be optimal for L-asparaginase production. Supplementation of glucose (0.1) and ammonium chloride (0.1) enhanced L-asparaginase production to 1.7 fold. Mixed substrate fermentation using soybean meal and wheat bran in the ratio (1:1 w/w) further enhanced production of L-asparaginase to 0.5 fold with a final yield of 18.91 U/gds. © 2017 Elsevier Lt

Growth inhibitory and proapoptotic effects of l-asparaginase from Fusarium culmorum ASP-87 on human leukemia cells (Jurkat)

The objective of this study was to evaluate the anticancer properties of l-asparaginase purified from fungal isolate Fusarium culmorum ASP-87 against human T-cell leukemia cell line (Jurkat). The growth inhibitory and proapoptotic effects of purified l-asparaginase on Jurkat cell lines were investigated by determining its influence on cell viability, colony formation, DNA fragmentation, and cell cycle progression. The results revealed that purified l-asparaginase showed significant decrease in cell survival with IC50 value of 90 μg/mL (9 IU/mL). The enzyme inhibited colony formation and showed characteristic laddering pattern on agarose gel thereby confirming the induction of apoptosis. Further, cell cycle analysis revealed that the enzyme induced apoptotic cell death by arresting the growth of cells at G2-M phase. However, the enzyme did not elicit any toxic effects on human erythrocytes. l-asparaginase purified from F. culmorum ASP-87 showed significant and selective cytotoxic and apoptotic effects on human T-cell leukemic cells in dose-dependent manner. Results of the study give leads for the anticancer effects of fungal l-asparaginase and its potential usefulness in the chemotherapy of leukemia. © 2016 Société Française de Pharmacologie et de Thérapeutiqu