4 research outputs found
The Impact of Surface Ligands and Synthesis Method on the Toxicity of Glutathione-Coated Gold Nanoparticles
Gold nanoparticles (AuNPs) are increasingly used in biomedical applications, hence understanding the processes that affect their biocompatibility and stability are of significant interest. In this study, we assessed the stability of peptide-capped AuNPs and used the embryonic zebrafish (Danio rerio) as a vertebrate system to investigate the impact of synthesis method and purity on their biocompatibility. Using glutathione (GSH) as a stabilizer, Au-GSH nanoparticles with identical core sizes were terminally modified with Tryptophan (Trp), Histidine (His) or Methionine (Met) amino acids and purified by either dialysis or ultracentrifugation. Au-GSH-(Trp)2 purified by dialysis elicited significant morbidity and mortality at 200 μg/mL, Au-GSH-(His)2 induced morbidity and mortality after purification by either method at 20 and 200 μg/mL, and Au-GSH-(Met)2 caused only sublethal responses at 200 μg/mL. Overall, toxicity was significantly reduced and ligand structure was improved by implementing ultracentrifugation purifications at several stages during the multi-step synthesis and surface modification of Au-GSH nanoparticles. When carefully synthesized at high purity, peptide-functionalized AuNPs showed high biocompatibility in biological systems
Numerical Simulations of Noisy Quantum Circuits for Computational Chemistry
The opportunities afforded by near-term quantum computers to calculate the
ground-state properties of small molecules depend on the structure of the
computational ansatz as well as the errors induced by device noise. Here we
investigate the behavior of these noisy quantum circuits using numerical
simulations to estimate the accuracy and fidelity of the prepared quantum
states relative to the ground truth obtained by conventional means. We
implement several different types of ansatz circuits derived from unitary
coupled cluster theory for the purposes of estimating the ground-state energy
of Sodium Hydride using the variational quantum eigensolver algorithm. We show
how relative error in the energy and the fidelity scale with the levels of
gate-based noise, the inter-molecular configuration, the ansatz circuit depth,
and the parameter optimization methods.Comment: 17 pages, 8 figure
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HarperBryanEMTImpactSurfaceLigands.pdf
Gold nanoparticles (AuNPs) are increasingly used in biomedical applications,
hence understanding the processes that affect their biocompatibility and stability are of
significant interest. In this study, we assessed the stability of peptide-capped AuNPs and
used the embryonic zebrafish (Danio rerio) as a vertebrate system to investigate the impact
of synthesis method and purity on their biocompatibility. Using glutathione (GSH) as a
stabilizer, Au-GSH nanoparticles with identical core sizes were terminally modified with
Tryptophan (Trp), Histidine (His) or Methionine (Met) amino acids and purified by either
dialysis or ultracentrifugation. Au-GSH-(Trp)â‚‚ purified by dialysis elicited significant
morbidity and mortality at 200 μg/mL, Au-GSH-(His)₂ induced morbidity and mortality
after purification by either method at 20 and 200 μg/mL, and Au-GSH-(Met)₂ caused only
sublethal responses at 200 μg/mL. Overall, toxicity was significantly reduced and ligand
structure was improved by implementing ultracentrifugation purifications at several stages
during the multi-step synthesis and surface modification of Au-GSH nanoparticles.
When carefully synthesized at high purity, peptide-functionalized AuNPs showed high
biocompatibility in biological systems.Keywords: synthesis, gold, purity, zebrafish, nanoparticle, biocompatibility, glutathione, nanotoxicityKeywords: synthesis, gold, purity, zebrafish, nanoparticle, biocompatibility, glutathione, nanotoxicit
Recommended from our members
HarperBryanEMTImpactSurfaceLigands_SupportingInformation.pdf
Gold nanoparticles (AuNPs) are increasingly used in biomedical applications,
hence understanding the processes that affect their biocompatibility and stability are of
significant interest. In this study, we assessed the stability of peptide-capped AuNPs and
used the embryonic zebrafish (Danio rerio) as a vertebrate system to investigate the impact
of synthesis method and purity on their biocompatibility. Using glutathione (GSH) as a
stabilizer, Au-GSH nanoparticles with identical core sizes were terminally modified with
Tryptophan (Trp), Histidine (His) or Methionine (Met) amino acids and purified by either
dialysis or ultracentrifugation. Au-GSH-(Trp)â‚‚ purified by dialysis elicited significant
morbidity and mortality at 200 μg/mL, Au-GSH-(His)₂ induced morbidity and mortality
after purification by either method at 20 and 200 μg/mL, and Au-GSH-(Met)₂ caused only
sublethal responses at 200 μg/mL. Overall, toxicity was significantly reduced and ligand
structure was improved by implementing ultracentrifugation purifications at several stages
during the multi-step synthesis and surface modification of Au-GSH nanoparticles.
When carefully synthesized at high purity, peptide-functionalized AuNPs showed high
biocompatibility in biological systems.Keywords: nanoparticle, gold, nanotoxicity, synthesis, biocompatibility, zebrafish, glutathione, purityKeywords: nanoparticle, gold, nanotoxicity, synthesis, biocompatibility, zebrafish, glutathione, purit