New signal processing method for multiplex pyrosequencing results analysis

Pyrosequencing is a DNA sequencing technology based on the sequencing-by-synthesis principle that has many applications including rapid genotyping of a broad spectrum of bacteria [1, 2]. Despite the increased use of Next Generation Sequencing for studying microbial diversity, pyrosequencing remains a cost-effective solution for genotyping short DNA stretches within bacterial genomes, hence providing rapid genetic assessment of specified microbial antibiotic resistance mechanisms [3,4]. In some cases, molecular typing may require to genotype multiple DNA stretches, each carrying a resistance determinant. Several pyrosequencing primers could be used simultaneously, with overlapping primer-specific pyrosequencing signals as main issue