The impact of an assessment toolkit on use of objective measurement tools in stroke rehabilitation.

Objective: To evaluate the impact of a toolkit of psychometrically robust measurement tools, the Greater Manchester Assessment for Stroke Rehabilitation (G-MASTER) toolkit, on the use of measurement tools during stroke rehabilitation Design: Mixed methods cohort design using non-participant observation of multi-disciplinary team meetings and semi-structured interviews with members of the team over three months before and three months after implementation of the assessment toolkit. Development and implementation of the toolkit are also described. Setting: Ten in-patient stroke services in a large UK city. Subjects: Members of the participating multi-disciplinary stroke teams. Results: Before implementation standardised measures were seldom used in team meetings. After implementation, use of all measurement tools significantly increased (36% to 81% of occasions, P&lt;0.000). Staff were generally positive about the toolkit and felt it enabled more accurate problem identification, effective progress monitoring, timely decision-making, communication and promoted inter-team relationships. Conclusions: A toolkit of standardised measurement tools can be feasibly and acceptably implemented into stroke rehabilitation. It increases the use of measurement tools by the multi-disciplinary team and improves the processes and quality of care. </jats:sec

Forced Fox-P3 expression can improve the safety and antigen-specific function of engineered regulatory T cells

Regulatory T cells (Treg) are potent inhibitors of autoreactive T cells. The intracellular transcription factor FoxP3 controls the expression levels of a diverse set of genes and plays a critical role in programming functional Tregs. Although, antigen-specific Tregs are more potent than polyclonal Tregs in treating ongoing autoimmunity, phenotype plasticity associated with loss of FoxP3 expression in Tregs can lead to the conversion into antigen-specific effector T cells which might exacerbate autoimmune pathology. In this study, we designed a retroviral vector driving the expression of FoxP3 and a human HLA-DR-restricted TCR from the same promoter. Transduction of purified human Tregs revealed that all TCR-positive cells had elevated levels of FoxP3 expression, increased CD25 and CTLA4 expression and potent suppressive function. Elevated FoxP3 expression did not impair the in vitro expansion of engineered Tregs. Adoptive transfer into HLA-DR transgenic mice revealed that FoxP3+TCR engineered Tregs showed long-term persistence with stable FoxP3 and TCR expression. In contrast, adoptive transfer of Tregs engineered with TCR only resulted in the accumulation of TCR-positive, FoxP3-negative T cells which displayed antigen-specific effector function when stimulated with the TCR-recognised peptides. Our data indicate that forced expression of FoxP3 can prevent accumulation of antigen-specific effector T cells without impairing the engraftment and persistence of engineered Tregs

Temporal and spatial variations in freshwater 14C reservoir effects: Lake Myvatn, Northern Iceland

Lake Mývatn is an interior highland lake in northern Iceland that forms a unique ecosystem of international scientific importance and is surrounded by a landscape rich in archaeological and palaeoenvironmental sites. A significant Freshwater 14C Reservoir Effect (FRE) has been identified in carbon from the lake at some Norse (c.870-1000 AD) archaeological sites in the wider region (Mývatnssveit). Previous AMS measurements indicated this FRE was ~1500-1900 14C years. Here we present the results of a study using stable isotope and 14C measurements to quantify the Mývatn FRE for both the Norse and modern periods. This work has identified a temporally variable FRE that is greatly in excess of previous assessments. New, paired samples of contemporaneous bone from terrestrial herbivores and omnivores (including humans) from Norse sites demonstrate at least some omnivore diets incorporated sufficient freshwater resources to result in a herbivore-omnivore age offset of up to 400 14C yrs. Modern samples of benthic detritus, aquatic plants, zooplankton, invertebrates and freshwater fish indicate an FRE in excess of 5000 14C yrs in some species. Likely geothermal mechanisms for this large FRE are discussed, along with implications for both chronological reconstruction and integrated investigation of stable and radioactive isotop

Identifying Young Brown Dwarfs Using Gravity-Sensitive Spectral Features

We report the initial results of the Brown Dwarf Spectroscopic Survey Gravity Project, to study gravity sensitive features as indicators of youth in brown dwarfs. Low-resolution (R~2000) J-band and optical (R~1000) observations using NIRSPEC and LRIS at the W.M. Keck Observatory reveal transitions of TiO, VO, K I, Na I, Cs I, Rb I, CaH, and FeH. By comparing these features in late-type giants and in old field dwarfs we show that they are sensitive to the gravity (g = GM/R^2) of the object. Using low-gravity spectral signatures as age indicators, we observed and analyzed J-band and optical spectra of two young brown dwarfs, G 196-3B (20-300 Myr) and KPNO Tau-4 (1-2 Myr), and two possible low mass brown dwarfs in the sigma Orionis cluster (3-7 Myr). We report the identification of the phi bands of TiO near 1.24 microns and the A-X band of VO near 1.18 microns together with extremely weak J-band lines of K I in KPNO-Tau4. This is the first detection of TiO and VO in the J-band in a sub-stellar mass object. The optical spectrum of KPNO-Tau4 exhibits weak K I and Na I lines, weak absorption by CaH, and strong VO bands, also signatures of a lower gravity atmosphere. G 196-3B shows absorption features in both wavelength regions like those of KPNO-Tau4 suggesting that its age and mass are at the lower end of published estimates. Whereas sigma Ori 51 appears to be consistent with a young sub-stellar object, sigma Ori 47 shows signatures of high gravity most closely resembling an old L1.5/L0, and can not be a member of the sigma Orionis cluster.Comment: 14 pages, 4 figures. To appear in the January 10, 2004 issue of the Astrophysical Journa

The NIRSPEC Brown Dwarf Spectroscopic Survey II: High-Resolution J-Band Spectra of M, L and T Dwarfs

We present a sequence of high resolution (R~20,000 or 15 km/s) infrared spectra of stars and brown dwarfs spanning spectral types M2.5 to T6. Observations of 16 objects were obtained using eight echelle orders to cover part of the J-band from 1.165-1.323 micron with NIRSPEC on the Keck II telescope. By comparing opacity plots and line lists, over 200 weak features in the J-band are identified with either FeH or H2O transitions. Absorption by FeH attains maximum strength in the mid-L dwarfs, while H2O absorption becomes systematically stronger towards later spectral types. Narrow resolved features broaden markedly after the M to L transition. Our high resolution spectra also reveal that the disappearance of neutral Al lines at the boundary between M and L dwarfs is remarkably abrupt, presumably because of the formation of grains. Neutral Fe lines can be traced to mid-L dwarfs before Fe is removed by condensation. The neutral potassium (K I) doublets that dominate the J-band have pressure broadened wings that continue to broaden from ~50 km/s (FWHM) at mid-M to ~500 km/s at mid-T. In contrast however, the measured pseudo-equivalent widths of these same lines reach a maximum in the mid-L dwarfs. The young L2 dwarf, G196-3B, exhibits narrow potassium lines without extensive pressure-broadened wings, indicative of a lower gravity atmosphere. Kelu-1AB, another L2, has exceptionally broad infrared lines, including FeH and H2O features, confirming its status as a rapid rotator. In contrast to other late T objects, the peculiar T6 dwarf 2MASS 0937+29 displays a complete absence of potassium even at high resolution, which may be a metallicity effect or a result of a cooler, higher-gravity atmosphere.Comment: 53 pages, 21 figures, data will be available at http://www.astro.ucla.edu/~mclean/BDSSarchive

The NIRSPEC Brown Dwarf Spectroscopic Survey. I. Low-Resolution Near-Infrared Spectra

We present the first results of a near-infrared (0.96-2.31 micron) spectroscopic survey of M, L, and T dwarfs obtained with NIRSPEC on the Keck II telescope. Our new survey has a resolving power of R = 2000 and is comprised of two major data sets: 53 J-band (1.14-1.36 micron) spectra covering all spectral types from M6 to T8 with at least two members in each spectral subclass (wherever possible), and 25 flux-calibrated spectra from 1.14 to 2.31 microns for most spectral classes between M6 and T8. Sixteen of these 25 objects have additional spectral coverage from 0.96-1.14 microns to provide overlap with optical spectra. Spectral flux ratio indexes for prominent molecular bands are derived and equivalent widths (EWs) for several atomic lines are measured. We find that a combination of four H2O and two CH4 band strengths can be used for spectral classification. Weak (EW~1-2 angstrom) atomic lines of Al I and Ca I disappear at the boundary between M and L types.Comment: 60 pages, 25 figures. To appear in the Astrophysical Journal Vol 596. Received 2003 March 31; accepted 2003 June 20. Web site at http://www.astro.ucla.edu/~mclean/BDSSarchiv

Discovery of a Very Young Field L Dwarf, 2MASS J01415823-4633574

While following up L dwarf candidates selected photometrically from the Two Micron All Sky Survey, we uncovered an unusual object designated 2MASS J01415823-4633574. Its optical spectrum exhibits very strong bands of vanadium oxide but abnormally weak absorptions by titanium oxide, potassium, and sodium. Morphologically such spectroscopic characteristics fall intermediate between old, field early-L dwarfs (log(g)~5) and very late M giants (log(g)~0), leading us to favor low gravity as the explanation for the unique spectral signatures of this L dwarf. Such a low gravity can be explained only if this L dwarf is much lower in mass than a typical old field L dwarf of similar temperature and is still contracting to its final radius. These conditions imply a very young age. Further evidence of youth is found in the near-infrared spectrum, including a triangular-shaped H-band continuum reminiscent of young brown dwarf candidates discovered in the Orion Nebula Cluster. Using the above information along with comparisons to brown dwarf atmospheric and interior models, our current best estimate is that this L dwarf has an age of 1-50 Myr and a mass of 6-25 M_Jupiter. The location of 2MASS 0141-4633 on the sky coupled with a distance estimate of ~35 pc and the above age estimate suggests that this object may be a brown dwarf member of either the 30-Myr-old Tucana/Horologium Association or the ~12-Myr-old beta Pic Moving Group.Comment: Accepted for publication in the 10 March 2006 issue (volume 639) of the Astrophysical Journa

Colour superconductivity in finite systems

In this paper we study the effect of finite size on the two-flavour colour superconducting state. As well as restricting the quarks to a box, we project onto states of good baryon number and onto colour singlets, these being necessary restrictions on any observable ``quark nuggets''. We find that whereas finite size alone has a significant effect for very small boxes, with the superconducting state often being destroyed, the effect of projection is to restore it again. The infinite-volume limit is a good approximation even for quite small systems.Comment: 14 pages RevTeX4, 12 eps figure