When good bugs go bad: Epidemiology and antimicrobial resistance profiles of Corynebacterium striatum, an emerging multidrug-resistant, opportunistic pathogen

ABSTRACT Infections with Corynebacterium striatum have been described in the literature over the last 2 decades, with the majority being bacteremia, central line infections, and occasionally, endocarditis. In recent years, the frequency of C. striatum infections appears to be increasing; a factor likely contributing to this is the increased ease and accuracy of the identification of Corynebacterium spp., including C. striatum , from clinical cultures. The objective of this study was to retrospectively characterize C. striatum isolates recovered from specimens submitted as part of routine patient care at a 1,250-bed, tertiary-care academic medical center. Multiple strain types were recovered, as demonstrated by repetitive-sequence-based PCR. Most of the strains of C. striatum characterized were resistant to antimicrobials commonly used to treat Gram-positive organisms, such as penicillin, ceftriaxone, meropenem, clindamycin, and tetracycline. The MIC 50 for ceftaroline was &gt;32 μg/ml. Although there are no interpretive criteria for susceptibility with telavancin, it appeared to have potent in vitro efficacy against this species, with MIC 50 and MIC 90 values of 0.064 and 0.125 μg/ml, respectively. Finally, as previously reported in case studies, we demonstrated rapid in vitro development of daptomycin resistance in 100% of the isolates tested ( n = 50), indicating that caution should be exhibited when using daptomycin for the treatment of C. striatum infections. C. striatum is an emerging, multidrug-resistant pathogen that can be associated with a variety of infection types. </jats:p

Influence of Logging on Douglas Fir Beetle Populations

All species of bark beetles of economic importance prefer to attack freshly-killed host material. Logging slash, wind-throw, and fire-killed timber provide ideal breeding grounds for bark beetles. A few species, mostly in the Dendroctonus group, are able to kill living trees. When beetles in the group, raised in preferred host material, cannot find any or enough freshly-killed trees, logs, or slash to enter, they may attack living trees. In the interior of British Columbia, infestations of the Douglas fir beetle can often be traced to logging disturbance

Using virtual spatial audio to aide visually impaired atheletes

Many people with visual impairments actively play soccer, however the task of making the game accessible is met with significant challenges. These challenges include: the need to constantly talk to signify location and detecting the positions of silent objects on the field. Our work aims to discover methods to help persons with visual impairments play soccer more efficiently and safely

The occurrence and control of the bruce spanworm in the Okanagan Valley, 1972

A minor outbreak of the Bruce spanworm, Operophtera bruceata (Hulst), occurred in fruit orchards of the Okanagan Valley in 1972. The heaviest infestations were limited to orchards where prebloom sprays for the fruittree leafroller, Archips argyrospilus (Walker), were neglected for two or more seasons. Prebloom applications of azinphosmethyl, diazinon or endosulfan at tight cluster bud to pink bud stage on apple gave good control. Apple, pear, cherry, apricot and plum were attacked

Comparison of sample preparation methods, instrumentation platforms, and contemporary commercial databases for identification of clinically relevant mycobacteria by matrix-assisted laser desorption ionization - Time of flight mass spectrometry

When mycobacteria are recovered in clinical specimens, timely species-level identification is required to establish the clinical significance of the isolate and facilitate optimization of antimicrobial therapy. Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has recently been reported to be a reliable and expedited method for identification of mycobacteria, although various specimen preparation techniques and databases for analysis are reported across studies. Here we compared two MALDI-TOF MS instrumentation platforms and three databases: Bruker Biotyper Real Time Classification 3.1 (Biotyper), Vitek MS Plus Saramis Premium (Saramis), and Vitek MS v3.0. We evaluated two sample preparation techniques and demonstrate that extraction methods are not interchangeable across different platforms or databases. Once testing parameters were established, a panel of 157 mycobacterial isolates (including 16 Mycobacterium tuberculosis isolates) was evaluated, demonstrating that with the appropriate specimen preparation, all three methods provide reliable identification for most species. Using a score cutoff value of ≥1.8, the Biotyper correctly identified 133 (84.7%) isolates with no misidentifications. Using a confidence value of ≥90%, Saramis correctly identified 134 (85.4%) isolates with one misidentification and Vitek MS v3.0 correctly identified 140 (89.2%) isolates with one misidentification. The levels of accuracy were not significantly different across the three platforms (P = 0.14). In addition, we show that Vitek MS v3.0 requires modestly fewer repeat analyses than the Biotyper and Saramis methods (P = 0.04), which may have implications for laboratory workflow

Brown-pigmented Mycobacterium mageritense as a cause of prosthetic valve endocarditis and bloodstream infection

Mycobacterium spp. are a rare cause of endocarditis. Herein, we describe a case of Mycobacterium mageritense prosthetic valve endocarditis. This organism produced an unusual brown pigment on solid media. Cultures of valve tissue for acid-fast bacilli might be considered in some cases of apparently culture-negative prosthetic valve endocarditis

A series of coverings of the regular n-gon

We define an infinite series of translation coverings of Veech's double-n-gon for odd n greater or equal to 5 which share the same Veech group. Additionally we give an infinite series of translation coverings with constant Veech group of a regular n-gon for even n greater or equal to 8. These families give rise to explicit examples of infinite translation surfaces with lattice Veech group.Comment: A missing case in step 1 in the proof of Thm. 1 b was added. (To appear in Geometriae Dedicata.

Edge pixel response studies of edgeless silicon sensor technology for pixellated imaging detectors

Silicon sensor technologies with reduced dead area at the sensor's perimeter are under development at a number of institutes. Several fabrication methods for sensors which are sensitive close to the physical edge of the device are under investigation utilising techniques such as active-edges, passivated edges and current-terminating rings. Such technologies offer the goal of a seamlessly tiled detection surface with minimum dead space between the individual modules. In order to quantify the performance of different geometries and different bulk and implant types, characterisation of several sensors fabricated using active-edge technology were performed at the B16 beam line of the Diamond Light Source. The sensors were fabricated by VTT and bump-bonded to Timepix ROICs. They were 100 and 200 μ m thick sensors, with the last pixel-to-edge distance of either 50 or 100 μ m. The sensors were fabricated as either n-on-n or n-on-p type devices. Using 15 keV monochromatic X-rays with a beam spot of 2.5 μ m, the performance at the outer edge and corners pixels of the sensors was evaluated at three bias voltages. The results indicate a significant change in the charge collection properties between the edge and 5th (up to 275 μ m) from edge pixel for the 200 μ m thick n-on-n sensor. The edge pixel performance of the 100 μ m thick n-on-p sensors is affected only for the last two pixels (up to 110 μ m) subject to biasing conditions. Imaging characteristics of all sensor types investigated are stable over time and the non-uniformities can be minimised by flat-field corrections. The results from the synchrotron tests combined with lab measurements are presented along with an explanation of the observed effects

Gott Time Machines, BTZ Black Hole Formation, and Choptuik Scaling

We study the formation of BTZ black holes by the collision of point particles. It is shown that the Gott time machine, originally constructed for the case of vanishing cosmological constant, provides a precise mechanism for black hole formation. As a result, one obtains an exact analytic understanding of the Choptuik scaling.Comment: 6 pages, Late

Bifurcations in the Space of Exponential Maps

This article investigates the parameter space of the exponential family $z\mapsto \exp(z)+\kappa$. We prove that the boundary (in \C) of every hyperbolic component is a Jordan arc, as conjectured by Eremenko and Lyubich as well as Baker and Rippon. In fact, we prove the stronger statement that the exponential bifurcation locus is connected in \C, which is an analog of Douady and Hubbard's celebrated theorem that the Mandelbrot set is connected. We show furthermore that $\infty$ is not accessible through any nonhyperbolic ("queer") stable component. The main part of the argument consists of demonstrating a general "Squeezing Lemma", which controls the structure of parameter space near infinity. We also prove a second conjecture of Eremenko and Lyubich concerning bifurcation trees of hyperbolic components.Comment: 29 pages, 3 figures. The main change in the new version is the introduction of Theorem 1.1 on the connectivity of the bifurcation locus, which follows from the results of the original version but was not explicitly stated. Also, some small revisions have been made and references update