843 research outputs found
X-ray diffraction from shock-loaded polycrystals
X-ray diffraction was demonstrated from shock-compressed polycrystalline
metal on nanosecond time scales. Laser ablation was used to induce shock waves
in polycrystalline foils of Be, 25 to 125 microns thick. A second laser pulse
was used to generate a plasma x-ray source by irradiation of a Ti foil. The
x-ray source was collimated to produce a beam of controllable diameter, and the
beam was directed at the Be sample. X-rays were diffracted from the sample, and
detected using films and x-ray streak cameras. The diffraction angle was
observed to change with shock pressure. The diffraction angles were consistent
with the uniaxial (elastic) and isotropic (plastic) compressions expected for
the loading conditions used. Polycrystalline diffraction will be used to
measure the response of the crystal lattice to high shock pressures and through
phase changes
Canadian Social Trends: A New Statistics Canada Social Statistics Publication
Canadian Social Trends: A New Statistics Canada Social Statistics Publicatio
A Remote Laser System for Material Characterization at High Temperatures
For many years, new techniques have been developed to overcome the problems involved with the generation and detection of ultrasound in materials at high temperature [1,2]. A non-contacting technique is described using lasers to generate and detect ultrasound and which can be used to study the variation in acoustic velocity as a function of temperature. Results are presented for the change in longitudinal velocity (vâ„“) with increasing temperature in five polycrystalline materials, namely Dural, aluminum, AISI-310 stainless steel, iron and graphite
Selective in vitro replication of herpes simplex virus type 1 (HSV-1) ICP34.5 null mutants in primary human CNS tumours--evaluation of a potentially effective clinical therapy.
Primary tumours of the central nervous system (CNS) are an important cause of cancer-related deaths in adults and children. CNS tumours are mostly glial cell in origin and are predominantly astrocytomas. Conventional therapy of high-grade gliomas includes maximal resection followed by radiation treatment. The addition of adjuvant chemotherapy provides little improvement in survival time and hence assessment of novel therapies is imperative. We have evaluated the potential therapeutic use of the herpes simplex virus (HSV) mutant 1716 in the treatment of primary brain tumours. The mutant is deleted in the RL1 gene and fails to produce the virulence factor ICP34.5. 1716 replication was analysed in both established human glioma cell lines and in primary cell cultures derived from human tumour biopsy material. In the majority of cultures, virus replication occurred and consequential cell death resulted. In the minority of tumour cell lines which are non-permissive for mutant replication, premature shut-off of host cell protein synthesis was induced in response to lack of expression of ICP34.5. Hence RL1-negative mutants have the distinct advantage of providing a double hit phenomenon whereby cell death could occur by either pathway. Moreover, 1716, by virtue of its ability to replicate selectively within a tumour cell, has the potential to deliver a 'suicide' gene product to the required site immediately. It is our opinion that HSV which fails to express ICP34.5 could provide an effective tumour therapy
C-Type natriuretic peptide: a potential urinary biomarker for renal remodeling and fibrosis during aging
Inhibition of macropinocytosis blocks antigen presentation of type II collagen in vitro and in vivo in HLA-DR1 transgenic mice
Professional antigen-presenting cells, such as dendritic cells, macrophages and B cells have been implicated in the pathogenesis of rheumatoid arthritis, constituting a possible target for antigen-specific immunotherapy. We addressed the possibility of blocking antigen presentation of the type II collagen (CII)-derived immunodominant arthritogenic epitope CII(259–273 )to specific CD4 T cells by inhibition of antigen uptake in HLA-DR1-transgenic mice in vitro and in vivo. Electron microscopy, confocal microscopy, subcellular fractionation and antigen presentation assays were used to establish the mechanisms of uptake, intracellular localization and antigen presentation of CII by dendritic cells and macrophages. We show that CII accumulated in membrane fractions of intermediate density corresponding to late endosomes. Treatment of dendritic cells and macrophages with cytochalasin D or amiloride prevented the intracellular appearance of CII and blocked antigen presentation of CII(259–273 )to HLA-DR1-restricted T cell hybridomas. The data suggest that CII was taken up by dendritic cells and macrophages predominantly via macropinocytosis. Administration of amiloride in vivo prevented activation of CII-specific polyclonal T cells in the draining popliteal lymph nodes. This study suggests that selective targeting of CII internalization in professional antigen-presenting cells prevents activation of autoimmune T cells, constituting a novel therapeutic strategy for the immunotherapy of rheumatoid arthritis
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