Synergistic Antitumor Activity of Vitamins C and K3 on Human Bladder Cancer Cell Lines

Copyright © 2013 Karen McGuire et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Exponentially growing cultures of human bladder tumor cells (RT4 and T24) were treated with Vitamin C (VC) alone, Vitamin K3 (VK3) alone, or with a VC:VK3 combination continuously for 5 days or treated with vitamins for 1 h, washed with PBS and then incubated in culture medium for 5 days. Co-administration of the vitamins enhanced the antitumor activity 12- to 24-fold for the RT-4 cells and 6- to 41-fold for the T24 cells. Flow cytometry of RT4 cells ex-posed to the vitamins revealed a growth arrested population and a population undergoing cell death. Growth arrested cells were blocked near the G0/G1-S-phase interface, while cell death was due to autoschizis. Catalase treatment abro-gated both cell cycle arrest and cell death which implicated hydrogen peroxide (H2O2) in these processes. The H2O2 production resulted in a moderate increase in lipid peroxidation and depletion of cell thiol levels. Analysis of cellular ATP levels revealed a transient increase in ATP production for VC and the VC:VK3 combination, but decreased ATP levels following VK3 treatment. Lipid peroxidation, thiol depletion and ATP modulation occurred at a 17-fold lower concentration in the vitamin combination than with either vitamin alone. These results suggested that the increased cy-totoxicity of the vitamin combination was due to redox cycling and increased oxidative stress

Streamflow response to increasing precipitation extremes altered by forest management

Published (Publication status

Implementation and performance of SIBYLS: a dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the Advanced Light Source.

The SIBYLS beamline (12.3.1) of the Advanced Light Source at Lawrence Berkeley National Laboratory, supported by the US Department of Energy and the National Institutes of Health, is optimized for both small-angle X-ray scattering (SAXS) and macromolecular crystallography (MX), making it unique among the world's mostly SAXS or MX dedicated beamlines. Since SIBYLS was commissioned, assessments of the limitations and advantages of a combined SAXS and MX beamline have suggested new strategies for integration and optimal data collection methods and have led to additional hardware and software enhancements. Features described include a dual mode monochromator [containing both Si(111) crystals and Mo/B(4)C multilayer elements], rapid beamline optics conversion between SAXS and MX modes, active beam stabilization, sample-loading robotics, and mail-in and remote data collection. These features allow users to gain valuable insights from both dynamic solution scattering and high-resolution atomic diffraction experiments performed at a single synchrotron beamline. Key practical issues considered for data collection and analysis include radiation damage, structural ensembles, alternative conformers and flexibility. SIBYLS develops and applies efficient combined MX and SAXS methods that deliver high-impact results by providing robust cost-effective routes to connect structures to biology and by performing experiments that aid beamline designs for next generation light sources

Perspectives from the water: Utilizing fisher’s observations to inform SNE/ MA windowpane science and managemen

Within fisheries, stakeholders often have varying viewpoints regarding natural marine resources, and use different sets information to evaluate their condition. Evaluating a resource with different sets of information can lead to different conclusions. Windowpane flounder (Scophthalmus aquosus) are a managed finfish species in the northwest Atlantic whose regulations have the potential to limit harvest opportunities for target species. We analyzed commercial trip and catch information from video data to understand local densities of windowpane flounder in conjunction with fisheries independent surveys. Video monitoring data from three Rhode Island commercial fisher’s vessels and fisheries independent trawl survey data were analyzed to understand the geographic distribution of the stock as well as overlap with temporary closed areas. Biomass data from the fisheries-dependent and fisheries-independent surveys were combined with a spatial-temporal model that accounted for differences in catchability among vessels and spatial autocorrelation. A separate analysis of esti-mated discard rates with observer data was also conducted to determine how the distribution of windowpane discards in Southern New England compared to the distribution of model predicted windowpane abundance. In agreement with the fishermen’s observations, the temporary closed areas were not located where the highest densities of windowpane flounder occurred. The temporary closed areas, however, were located where the highest rates of discards occurred and thus where fishing had the greatest impact on the stock. The integration of verified fishery-dependent data with the scientific surveys has the potential to create a single set of information that is trusted by all user groups

Estimating Northern Bobwhite Density in Privately-Owned Forests Across the Southeast

Rigorous density estimates can inform management, conservation planning, and policy decisions. Northern bobwhite (Colinus virginianus; hereafter, bobwhite) populations are declining throughout their range, including the southeastern United States. Numerous private land conservation initiatives are underway to restore bobwhite populations in these areas, but baseline estimates of density spanning privately owned forests are sparse. This information gap makes it difficult to evaluate the population-level effects of these conservation programs or develop expectations for timelines to reach population targets. We sought to understand baseline densities across privately owned pine forests in the southeastern United States in areas targeted for bobwhite conservation. We sampled 138 pine stands a total of 286 times across Alabama, Florida, Georgia, North Carolina, and South Carolina from 2018 to 2020 using autumn distance sampling point counts. We sampled 110 stands that were either planned or applied U.S. Department of Agriculture, Natural Resources Conservation Service (NRCS) management contracts, specifically Working Lands for Wildlife (WLFW) Northern Bobwhite partnerships or similar Environmental Quality Incentives Program contracts. The WLFW contracts were solely in focal counties identified in the National Bobwhite Conservation Initiative 2.0 plan and by cooperating state wildlife agencies. Contracts were on average 17.4 ha (SD = 27.0). We also sampled 28 areas with extensive (\u3e200 ha) and intensive (e.g., at least every 3 years for the last ~10 years) habitat management for bobwhite. We used a hierarchical distance sampling model to estimate density. Density was lowest in North Carolina and South Carolina. Densities in Alabama were slightly greater, and greatest densities were in Georgia and Florida. However, the majority of observed densities were 2 coveys/40 ha or below, and all were below 4 coveys/40 ha. Large and intensively managed sites on average had 4 times greater density compared to smaller contracts. Specific densities will be reported in the future. Our sampling efforts fill an important information gap regarding densities throughout private lands in the southeastern United States. Our models indicate that bobwhite densities are generally low in pine forests of the Southeast even in counties identified as having the greatest conservation potential. The relatively greater densities at large and intensively managed sites illustrate the need for more intensive landscape-scale planning when implementing private land conservation. Pending research will compare densities at planned and applied NRCS contracts in the context of landscape-scale habitat amount

Neuropsychological Outcomes of U.S. Veterans with Report of Remote Blast-Related Concussion and Current Psychopathology

This study explored whether remote blast-related MTBI and/or current Axis I psychopathology contribute to neuropsychological outcomes among OEF/OIF veterans with varied combat histories. OEF/OIF veterans underwent structured interviews to evaluate history of blast-related MTBI and psychopathology and were assigned to MTBI (n = 18), Axis I (n = 24), Co-morbid MTBI/Axis I (n = 34), or post-deployment control (n = 28) groups. A main effect for Axis I diagnosis on overall neuropsychological performance was identified (F(3,100) = 4.81; p = .004), with large effect sizes noted for the Axis I only (d = .98) and Co-morbid MTBI/Axis I (d = .95) groups relative to the control group. The latter groups demonstrated primary limitations on measures of learning/memory and processing speed. The MTBI only group demonstrated performances that were not significantly different from the remaining three groups. These findings suggest that a remote history of blast-related MTBI does not contribute to objective cognitive impairment in the late stage of injury. Impairments, when present, are subtle and most likely attributable to PTSD and other psychological conditions. Implications for clinical neuropsychologists and future research are discussed. (JINS, 2012, 18, 1–11

What really matters about teacher education at Cathedrals Group Universities: volume 2 the case studies

The case studies show insight into the extent that there is a shared understanding between schools, students and staff members in some of England’s oldest providers of teacher education in England. Is there something particular about that provision? Could it be described as distinctively, implicitly or explicitly Christian? Is there a sense of shared thinking about the answers to these questions in the provision of teacher education and the students, university tutors and school staff members who partner with these universities to educate the next generation of teachers? This document provides five answers to those questions. The answers are snapshots of the perception of teacher education at these universities, at a time when teacher education has become a major purpose of schools, and universities have found themselves being questioned and challenged about their role in the development of new teachers

Insights and issues with simulating terrestrial DOC loading of Arctic river networks

Author Posting. © Ecological Society of America, 2013. This article is posted here by permission of Ecological Society of America for personal use, not for redistribution. The definitive version was published in Ecological Applications 23 (2013): 1817-1836, doi:10.1890/11-1050.1.Terrestrial carbon dynamics influence the contribution of dissolved organic carbon (DOC) to river networks in addition to hydrology. In this study, we use a biogeochemical process model to simulate the lateral transfer of DOC from land to the Arctic Ocean via riverine transport. We estimate that, over the 20th century, the pan-Arctic watershed has contributed, on average, 32 Tg C/yr of DOC to river networks emptying into the Arctic Ocean with most of the DOC coming from the extensive area of boreal deciduous needle-leaved forests and forested wetlands in Eurasian watersheds. We also estimate that the rate of terrestrial DOC loading has been increasing by 0.037 Tg C/yr2 over the 20th century primarily as a result of climate-induced increases in water yield. These increases have been offset by decreases in terrestrial DOC loading caused by wildfires. Other environmental factors (CO2 fertilization, ozone pollution, atmospheric nitrogen deposition, timber harvest, agriculture) are estimated to have relatively small effects on terrestrial DOC loading to Arctic rivers. The effects of the various environmental factors on terrestrial carbon dynamics have both offset and enhanced concurrent effects on hydrology to influence terrestrial DOC loading and may be changing the relative importance of terrestrial carbon dynamics on this carbon flux. Improvements in simulating terrestrial DOC loading to pan-Arctic rivers in the future will require better information on the production and consumption of DOC within the soil profile, the transfer of DOC from land to headwater streams, the spatial distribution of precipitation and its temporal trends, carbon dynamics of larch-dominated ecosystems in eastern Siberia, and the role of industrial organic effluents on carbon budgets of rivers in western Russia.This study was supported, in part, by the U.S. National Science Foundation under grants ARC-0531047, ARC-0531082, ARC-0531119, ARC-0554811, and ARC- 0652838; the U.S. Environmental Protection Agency under grant R833261; the U.S. Department of Energy under grant DE-FG02-08ER64597; and the U.S. National Aeronautics and Space Administration under grant NNX09A126G

Ketamine-Induced Disruption of Verbal Self-Monitoring Linked to Superior Temporal Activation

Introduction: Misattribution of distorted self-generated speech in patients with schizophrenia has been associated with increased lateral temporal activation. As a pharmacological model of schizophrenia, we tested whether ketamine would induce the same effects in healthy individuals. Methods: Participants were 8 healthy male volunteers who were nave to ketamine (mean age: 28 years). Ketamine (0.23 mg/kg bolus followed by 0.64 mg/kg/h) and placebo infusions were administered in a double-blind, randomised order, during 2 functional magnetic resonance imaging (fMRI) sessions. Each fMRI session consisted of a verbal self-monitoring task in which auditory feedback was experimentally modified. Results: Ketamine was associated with psychotic and dissociative symptoms. Participants made more misattributions of distorted self-generated speech (p <0.02) during the ketamine infusion. Ketamine led to reduced activation in the left superior temporal cortex during self-distorted speech, regardless of whether the speech was identified correctly or not, as compared to the placebo infusion. Misidentification of speech that had been distorted was not associated with any increase in brain activation in during the placebo infusion, however ketamine-induced misattributions were associated with a relative increase in left superior temporal cortex activation. Discussion: These data are consistent with the notion that self-monitoring impairments underlie psychotic symptoms and suggest that N-methyl-D-aspartate (NMDA) receptor dysfunction may mediate self-monitoring deficits and psychotic phenomena in schizophrenia