18 research outputs found
Bed and Breakfasts, Small Inns, and the Internet: The Impact of Technology on the Globalization of Small Businesses
Competition between annual weeds and vining peas grown at a range of population densities: effects on the weeds
Competition between annual weeds and vining peas grown at a range of population densities: effects on the crop
A chromatographic method for the production of a human immunoglobulin G solution for intravenous use
Immunoglobulin G (IgG) of excellent quality for intravenous use was obtained from the cryosupernatant of human plasma by a chromatographic method based on a mixture of ion-exchange, DEAE-Sepharose FF and arginine Sepharose 4B affinity chromatography and a final purification step by Sephacryl S-300 HR gel filtration. The yield of 10 experimental batches produced was 3.5 g IgG per liter of plasma. A solvent/detergent combination of 1% Tri (n-butyl) phosphate and 1% Triton X-100 was used to inactivate lipid-coated viruses. Analysis of the final product (5% liquid IgG) based on the mean for 10 batches showed 94% monomers, 5.5% dimers and 0.5% polymers and aggregates. Anticomplementary activity was 0.3 CH50/mg IgG and prekallikrein activator levels were less than 5 IU/ml. Stability at 37ºC for 30 days in the liquid state was satisfactory. IgG was stored in flasks (2.5 g/flask) at 4 to 8ºC. All the characteristics of the product were consistent with the requirements of the 1997 Pharmacopée Européenne
The Part-Time Wage Gap: a Career Perspective
part-time employment, promotions, wages, wage gap, J31, J24, J22,